New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Acta Amazonica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305 |
Resumo: | ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon. |
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New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detectionDidelphimorphiaRodentialive trapskidneyliver16S rRNAABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.Instituto Nacional de Pesquisas da Amazônia2020-12-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305Acta Amazonica v.50 n.4 2020reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201903683info:eu-repo/semantics/openAccessSOUZA ROCHA,Katarine deSCHUPP DE SENA MESQUITA,GleicianeSILVA FERREIRA,Maeli FernandaLEITE BARROS,Flávia de NazaréMACEDO,Renata Cecília Soares de LimaARAÚJO SARAIVA,Elane deMENDES-OLIVEIRA,Ana CristinaDUARTE CERQUEIRA,ValíriaSCOFIELD,AlessandraGOES CAVALCANTE,GustavoABEL,IsisGUIMARÃES DE MORAES,Carla Cristinaeng2020-12-04T00:00:00Zoai:scielo:S0044-59672020000400305Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2020-12-04T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.none.fl_str_mv |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
title |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
spellingShingle |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection SOUZA ROCHA,Katarine de Didelphimorphia Rodentia live traps kidney liver 16S rRNA |
title_short |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
title_full |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
title_fullStr |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
title_full_unstemmed |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
title_sort |
New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection |
author |
SOUZA ROCHA,Katarine de |
author_facet |
SOUZA ROCHA,Katarine de SCHUPP DE SENA MESQUITA,Gleiciane SILVA FERREIRA,Maeli Fernanda LEITE BARROS,Flávia de Nazaré MACEDO,Renata Cecília Soares de Lima ARAÚJO SARAIVA,Elane de MENDES-OLIVEIRA,Ana Cristina DUARTE CERQUEIRA,Valíria SCOFIELD,Alessandra GOES CAVALCANTE,Gustavo ABEL,Isis GUIMARÃES DE MORAES,Carla Cristina |
author_role |
author |
author2 |
SCHUPP DE SENA MESQUITA,Gleiciane SILVA FERREIRA,Maeli Fernanda LEITE BARROS,Flávia de Nazaré MACEDO,Renata Cecília Soares de Lima ARAÚJO SARAIVA,Elane de MENDES-OLIVEIRA,Ana Cristina DUARTE CERQUEIRA,Valíria SCOFIELD,Alessandra GOES CAVALCANTE,Gustavo ABEL,Isis GUIMARÃES DE MORAES,Carla Cristina |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
SOUZA ROCHA,Katarine de SCHUPP DE SENA MESQUITA,Gleiciane SILVA FERREIRA,Maeli Fernanda LEITE BARROS,Flávia de Nazaré MACEDO,Renata Cecília Soares de Lima ARAÚJO SARAIVA,Elane de MENDES-OLIVEIRA,Ana Cristina DUARTE CERQUEIRA,Valíria SCOFIELD,Alessandra GOES CAVALCANTE,Gustavo ABEL,Isis GUIMARÃES DE MORAES,Carla Cristina |
dc.subject.por.fl_str_mv |
Didelphimorphia Rodentia live traps kidney liver 16S rRNA |
topic |
Didelphimorphia Rodentia live traps kidney liver 16S rRNA |
description |
ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1809-4392201903683 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
Acta Amazonica v.50 n.4 2020 reponame:Acta Amazonica instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Acta Amazonica |
collection |
Acta Amazonica |
repository.name.fl_str_mv |
Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
acta@inpa.gov.br||acta@inpa.gov.br |
_version_ |
1752129841257250816 |