New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection

Detalhes bibliográficos
Autor(a) principal: SOUZA ROCHA,Katarine de
Data de Publicação: 2020
Outros Autores: SCHUPP DE SENA MESQUITA,Gleiciane, SILVA FERREIRA,Maeli Fernanda, LEITE BARROS,Flávia de Nazaré, MACEDO,Renata Cecília Soares de Lima, ARAÚJO SARAIVA,Elane de, MENDES-OLIVEIRA,Ana Cristina, DUARTE CERQUEIRA,Valíria, SCOFIELD,Alessandra, GOES CAVALCANTE,Gustavo, ABEL,Isis, GUIMARÃES DE MORAES,Carla Cristina
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Acta Amazonica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305
Resumo: ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.
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spelling New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detectionDidelphimorphiaRodentialive trapskidneyliver16S rRNAABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.Instituto Nacional de Pesquisas da Amazônia2020-12-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305Acta Amazonica v.50 n.4 2020reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201903683info:eu-repo/semantics/openAccessSOUZA ROCHA,Katarine deSCHUPP DE SENA MESQUITA,GleicianeSILVA FERREIRA,Maeli FernandaLEITE BARROS,Flávia de NazaréMACEDO,Renata Cecília Soares de LimaARAÚJO SARAIVA,Elane deMENDES-OLIVEIRA,Ana CristinaDUARTE CERQUEIRA,ValíriaSCOFIELD,AlessandraGOES CAVALCANTE,GustavoABEL,IsisGUIMARÃES DE MORAES,Carla Cristinaeng2020-12-04T00:00:00Zoai:scielo:S0044-59672020000400305Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2020-12-04T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.none.fl_str_mv New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
title New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
spellingShingle New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
SOUZA ROCHA,Katarine de
Didelphimorphia
Rodentia
live traps
kidney
liver
16S rRNA
title_short New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
title_full New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
title_fullStr New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
title_full_unstemmed New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
title_sort New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection
author SOUZA ROCHA,Katarine de
author_facet SOUZA ROCHA,Katarine de
SCHUPP DE SENA MESQUITA,Gleiciane
SILVA FERREIRA,Maeli Fernanda
LEITE BARROS,Flávia de Nazaré
MACEDO,Renata Cecília Soares de Lima
ARAÚJO SARAIVA,Elane de
MENDES-OLIVEIRA,Ana Cristina
DUARTE CERQUEIRA,Valíria
SCOFIELD,Alessandra
GOES CAVALCANTE,Gustavo
ABEL,Isis
GUIMARÃES DE MORAES,Carla Cristina
author_role author
author2 SCHUPP DE SENA MESQUITA,Gleiciane
SILVA FERREIRA,Maeli Fernanda
LEITE BARROS,Flávia de Nazaré
MACEDO,Renata Cecília Soares de Lima
ARAÚJO SARAIVA,Elane de
MENDES-OLIVEIRA,Ana Cristina
DUARTE CERQUEIRA,Valíria
SCOFIELD,Alessandra
GOES CAVALCANTE,Gustavo
ABEL,Isis
GUIMARÃES DE MORAES,Carla Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SOUZA ROCHA,Katarine de
SCHUPP DE SENA MESQUITA,Gleiciane
SILVA FERREIRA,Maeli Fernanda
LEITE BARROS,Flávia de Nazaré
MACEDO,Renata Cecília Soares de Lima
ARAÚJO SARAIVA,Elane de
MENDES-OLIVEIRA,Ana Cristina
DUARTE CERQUEIRA,Valíria
SCOFIELD,Alessandra
GOES CAVALCANTE,Gustavo
ABEL,Isis
GUIMARÃES DE MORAES,Carla Cristina
dc.subject.por.fl_str_mv Didelphimorphia
Rodentia
live traps
kidney
liver
16S rRNA
topic Didelphimorphia
Rodentia
live traps
kidney
liver
16S rRNA
description ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400305
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1809-4392201903683
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
dc.source.none.fl_str_mv Acta Amazonica v.50 n.4 2020
reponame:Acta Amazonica
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Acta Amazonica
collection Acta Amazonica
repository.name.fl_str_mv Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv acta@inpa.gov.br||acta@inpa.gov.br
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