In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Amazonica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334 |
Resumo: | ABSTRACT Malaria is a disease of global tropical distribution, being endemic in more than 90 countries and responsible for about 212 million cases worldwide in 2016. To date, the strategies used to eradicate this disease have been ineffective, without specific preventive measures such as vaccines. Currently, the existing therapeutic arsenal is limited and has become ineffective against the expansion of artemisinin-resistant Plasmodium, demonstrating the need for studies that would allow the development of new compounds against this disease. In this context, we studied the volatile oil obtained from rhizomes of Cyperus articulatus (VOCA), a plant species commonly found in the Amazon region and popularly used as a therapeutic alternative for the treatment of malaria, in order to confirm its potential as an antimalarial agent by in vitro and in vivo assays. We cultured Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains in erythrocytes and exposed them to VOCA at different concentrations in 96-well microplates. In vivo antimalarial activity was tested in BALB/c mice inoculated with approximately 106 erythrocytes infected with Plasmodium berghei. VOCA showed a high antimalarial potential against the two P. falciparum strains, with IC50 = 1.21 μg mL-1 for W2 and 2.30 μg mL-1 for 3D7. VOCA also significantly reduced the parasitemia and anemia induced by P. berghei in mice. Our results confirmed the antimalarial potential of the volatile oil of Cyperus articulatus. |
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In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)antiplasmodialartemisinin resistancechloroquine resistancemalariaPlasmodium falciparumPlasmodium bergheiABSTRACT Malaria is a disease of global tropical distribution, being endemic in more than 90 countries and responsible for about 212 million cases worldwide in 2016. To date, the strategies used to eradicate this disease have been ineffective, without specific preventive measures such as vaccines. Currently, the existing therapeutic arsenal is limited and has become ineffective against the expansion of artemisinin-resistant Plasmodium, demonstrating the need for studies that would allow the development of new compounds against this disease. In this context, we studied the volatile oil obtained from rhizomes of Cyperus articulatus (VOCA), a plant species commonly found in the Amazon region and popularly used as a therapeutic alternative for the treatment of malaria, in order to confirm its potential as an antimalarial agent by in vitro and in vivo assays. We cultured Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains in erythrocytes and exposed them to VOCA at different concentrations in 96-well microplates. In vivo antimalarial activity was tested in BALB/c mice inoculated with approximately 106 erythrocytes infected with Plasmodium berghei. VOCA showed a high antimalarial potential against the two P. falciparum strains, with IC50 = 1.21 μg mL-1 for W2 and 2.30 μg mL-1 for 3D7. VOCA also significantly reduced the parasitemia and anemia induced by P. berghei in mice. Our results confirmed the antimalarial potential of the volatile oil of Cyperus articulatus.Instituto Nacional de Pesquisas da Amazônia2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334Acta Amazonica v.49 n.4 2019reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201804331info:eu-repo/semantics/openAccessSILVA,Nazaré Carneiro daGONÇALVES,Suellen FerreiraARAÚJO,Luciana Silva deKASPER,Aline Aparecida MünchenFONSECA,Amanda Luisa daSARTORATTO,AdilsonCASTRO,Kelly Christina FerreiraMORAES,Tânia Mara PiresBARATTO,Leopoldo ClementeVAROTTI,Fernando de PillaBARATA,Lauro Euclides SoaresMORAES,Waldiney Pireseng2019-11-06T00:00:00Zoai:scielo:S0044-59672019000400334Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2019-11-06T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.none.fl_str_mv |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
title |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
spellingShingle |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) SILVA,Nazaré Carneiro da antiplasmodial artemisinin resistance chloroquine resistance malaria Plasmodium falciparum Plasmodium berghei |
title_short |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
title_full |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
title_fullStr |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
title_full_unstemmed |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
title_sort |
In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae) |
author |
SILVA,Nazaré Carneiro da |
author_facet |
SILVA,Nazaré Carneiro da GONÇALVES,Suellen Ferreira ARAÚJO,Luciana Silva de KASPER,Aline Aparecida München FONSECA,Amanda Luisa da SARTORATTO,Adilson CASTRO,Kelly Christina Ferreira MORAES,Tânia Mara Pires BARATTO,Leopoldo Clemente VAROTTI,Fernando de Pilla BARATA,Lauro Euclides Soares MORAES,Waldiney Pires |
author_role |
author |
author2 |
GONÇALVES,Suellen Ferreira ARAÚJO,Luciana Silva de KASPER,Aline Aparecida München FONSECA,Amanda Luisa da SARTORATTO,Adilson CASTRO,Kelly Christina Ferreira MORAES,Tânia Mara Pires BARATTO,Leopoldo Clemente VAROTTI,Fernando de Pilla BARATA,Lauro Euclides Soares MORAES,Waldiney Pires |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
SILVA,Nazaré Carneiro da GONÇALVES,Suellen Ferreira ARAÚJO,Luciana Silva de KASPER,Aline Aparecida München FONSECA,Amanda Luisa da SARTORATTO,Adilson CASTRO,Kelly Christina Ferreira MORAES,Tânia Mara Pires BARATTO,Leopoldo Clemente VAROTTI,Fernando de Pilla BARATA,Lauro Euclides Soares MORAES,Waldiney Pires |
dc.subject.por.fl_str_mv |
antiplasmodial artemisinin resistance chloroquine resistance malaria Plasmodium falciparum Plasmodium berghei |
topic |
antiplasmodial artemisinin resistance chloroquine resistance malaria Plasmodium falciparum Plasmodium berghei |
description |
ABSTRACT Malaria is a disease of global tropical distribution, being endemic in more than 90 countries and responsible for about 212 million cases worldwide in 2016. To date, the strategies used to eradicate this disease have been ineffective, without specific preventive measures such as vaccines. Currently, the existing therapeutic arsenal is limited and has become ineffective against the expansion of artemisinin-resistant Plasmodium, demonstrating the need for studies that would allow the development of new compounds against this disease. In this context, we studied the volatile oil obtained from rhizomes of Cyperus articulatus (VOCA), a plant species commonly found in the Amazon region and popularly used as a therapeutic alternative for the treatment of malaria, in order to confirm its potential as an antimalarial agent by in vitro and in vivo assays. We cultured Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains in erythrocytes and exposed them to VOCA at different concentrations in 96-well microplates. In vivo antimalarial activity was tested in BALB/c mice inoculated with approximately 106 erythrocytes infected with Plasmodium berghei. VOCA showed a high antimalarial potential against the two P. falciparum strains, with IC50 = 1.21 μg mL-1 for W2 and 2.30 μg mL-1 for 3D7. VOCA also significantly reduced the parasitemia and anemia induced by P. berghei in mice. Our results confirmed the antimalarial potential of the volatile oil of Cyperus articulatus. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1809-4392201804331 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
Acta Amazonica v.49 n.4 2019 reponame:Acta Amazonica instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Acta Amazonica |
collection |
Acta Amazonica |
repository.name.fl_str_mv |
Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
acta@inpa.gov.br||acta@inpa.gov.br |
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1752129841179656192 |