In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)

Detalhes bibliográficos
Autor(a) principal: SILVA,Nazaré Carneiro da
Data de Publicação: 2019
Outros Autores: GONÇALVES,Suellen Ferreira, ARAÚJO,Luciana Silva de, KASPER,Aline Aparecida München, FONSECA,Amanda Luisa da, SARTORATTO,Adilson, CASTRO,Kelly Christina Ferreira, MORAES,Tânia Mara Pires, BARATTO,Leopoldo Clemente, VAROTTI,Fernando de Pilla, BARATA,Lauro Euclides Soares, MORAES,Waldiney Pires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Amazonica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334
Resumo: ABSTRACT Malaria is a disease of global tropical distribution, being endemic in more than 90 countries and responsible for about 212 million cases worldwide in 2016. To date, the strategies used to eradicate this disease have been ineffective, without specific preventive measures such as vaccines. Currently, the existing therapeutic arsenal is limited and has become ineffective against the expansion of artemisinin-resistant Plasmodium, demonstrating the need for studies that would allow the development of new compounds against this disease. In this context, we studied the volatile oil obtained from rhizomes of Cyperus articulatus (VOCA), a plant species commonly found in the Amazon region and popularly used as a therapeutic alternative for the treatment of malaria, in order to confirm its potential as an antimalarial agent by in vitro and in vivo assays. We cultured Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains in erythrocytes and exposed them to VOCA at different concentrations in 96-well microplates. In vivo antimalarial activity was tested in BALB/c mice inoculated with approximately 106 erythrocytes infected with Plasmodium berghei. VOCA showed a high antimalarial potential against the two P. falciparum strains, with IC50 = 1.21 μg mL-1 for W2 and 2.30 μg mL-1 for 3D7. VOCA also significantly reduced the parasitemia and anemia induced by P. berghei in mice. Our results confirmed the antimalarial potential of the volatile oil of Cyperus articulatus.
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spelling In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)antiplasmodialartemisinin resistancechloroquine resistancemalariaPlasmodium falciparumPlasmodium bergheiABSTRACT Malaria is a disease of global tropical distribution, being endemic in more than 90 countries and responsible for about 212 million cases worldwide in 2016. To date, the strategies used to eradicate this disease have been ineffective, without specific preventive measures such as vaccines. Currently, the existing therapeutic arsenal is limited and has become ineffective against the expansion of artemisinin-resistant Plasmodium, demonstrating the need for studies that would allow the development of new compounds against this disease. In this context, we studied the volatile oil obtained from rhizomes of Cyperus articulatus (VOCA), a plant species commonly found in the Amazon region and popularly used as a therapeutic alternative for the treatment of malaria, in order to confirm its potential as an antimalarial agent by in vitro and in vivo assays. We cultured Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains in erythrocytes and exposed them to VOCA at different concentrations in 96-well microplates. In vivo antimalarial activity was tested in BALB/c mice inoculated with approximately 106 erythrocytes infected with Plasmodium berghei. VOCA showed a high antimalarial potential against the two P. falciparum strains, with IC50 = 1.21 μg mL-1 for W2 and 2.30 μg mL-1 for 3D7. VOCA also significantly reduced the parasitemia and anemia induced by P. berghei in mice. Our results confirmed the antimalarial potential of the volatile oil of Cyperus articulatus.Instituto Nacional de Pesquisas da Amazônia2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334Acta Amazonica v.49 n.4 2019reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201804331info:eu-repo/semantics/openAccessSILVA,Nazaré Carneiro daGONÇALVES,Suellen FerreiraARAÚJO,Luciana Silva deKASPER,Aline Aparecida MünchenFONSECA,Amanda Luisa daSARTORATTO,AdilsonCASTRO,Kelly Christina FerreiraMORAES,Tânia Mara PiresBARATTO,Leopoldo ClementeVAROTTI,Fernando de PillaBARATA,Lauro Euclides SoaresMORAES,Waldiney Pireseng2019-11-06T00:00:00Zoai:scielo:S0044-59672019000400334Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2019-11-06T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false
dc.title.none.fl_str_mv In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
title In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
spellingShingle In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
SILVA,Nazaré Carneiro da
antiplasmodial
artemisinin resistance
chloroquine resistance
malaria
Plasmodium falciparum
Plasmodium berghei
title_short In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
title_full In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
title_fullStr In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
title_full_unstemmed In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
title_sort In vitro and in vivo antimalarial activity of the volatile oil of Cyperus articulatus (Cyperaceae)
author SILVA,Nazaré Carneiro da
author_facet SILVA,Nazaré Carneiro da
GONÇALVES,Suellen Ferreira
ARAÚJO,Luciana Silva de
KASPER,Aline Aparecida München
FONSECA,Amanda Luisa da
SARTORATTO,Adilson
CASTRO,Kelly Christina Ferreira
MORAES,Tânia Mara Pires
BARATTO,Leopoldo Clemente
VAROTTI,Fernando de Pilla
BARATA,Lauro Euclides Soares
MORAES,Waldiney Pires
author_role author
author2 GONÇALVES,Suellen Ferreira
ARAÚJO,Luciana Silva de
KASPER,Aline Aparecida München
FONSECA,Amanda Luisa da
SARTORATTO,Adilson
CASTRO,Kelly Christina Ferreira
MORAES,Tânia Mara Pires
BARATTO,Leopoldo Clemente
VAROTTI,Fernando de Pilla
BARATA,Lauro Euclides Soares
MORAES,Waldiney Pires
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SILVA,Nazaré Carneiro da
GONÇALVES,Suellen Ferreira
ARAÚJO,Luciana Silva de
KASPER,Aline Aparecida München
FONSECA,Amanda Luisa da
SARTORATTO,Adilson
CASTRO,Kelly Christina Ferreira
MORAES,Tânia Mara Pires
BARATTO,Leopoldo Clemente
VAROTTI,Fernando de Pilla
BARATA,Lauro Euclides Soares
MORAES,Waldiney Pires
dc.subject.por.fl_str_mv antiplasmodial
artemisinin resistance
chloroquine resistance
malaria
Plasmodium falciparum
Plasmodium berghei
topic antiplasmodial
artemisinin resistance
chloroquine resistance
malaria
Plasmodium falciparum
Plasmodium berghei
description ABSTRACT Malaria is a disease of global tropical distribution, being endemic in more than 90 countries and responsible for about 212 million cases worldwide in 2016. To date, the strategies used to eradicate this disease have been ineffective, without specific preventive measures such as vaccines. Currently, the existing therapeutic arsenal is limited and has become ineffective against the expansion of artemisinin-resistant Plasmodium, demonstrating the need for studies that would allow the development of new compounds against this disease. In this context, we studied the volatile oil obtained from rhizomes of Cyperus articulatus (VOCA), a plant species commonly found in the Amazon region and popularly used as a therapeutic alternative for the treatment of malaria, in order to confirm its potential as an antimalarial agent by in vitro and in vivo assays. We cultured Plasmodium falciparum W2 (chloroquine-resistant) and 3D7 (chloroquine-sensitive) strains in erythrocytes and exposed them to VOCA at different concentrations in 96-well microplates. In vivo antimalarial activity was tested in BALB/c mice inoculated with approximately 106 erythrocytes infected with Plasmodium berghei. VOCA showed a high antimalarial potential against the two P. falciparum strains, with IC50 = 1.21 μg mL-1 for W2 and 2.30 μg mL-1 for 3D7. VOCA also significantly reduced the parasitemia and anemia induced by P. berghei in mice. Our results confirmed the antimalarial potential of the volatile oil of Cyperus articulatus.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000400334
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1809-4392201804331
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
publisher.none.fl_str_mv Instituto Nacional de Pesquisas da Amazônia
dc.source.none.fl_str_mv Acta Amazonica v.49 n.4 2019
reponame:Acta Amazonica
instname:Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron:INPA
instname_str Instituto Nacional de Pesquisas da Amazônia (INPA)
instacron_str INPA
institution INPA
reponame_str Acta Amazonica
collection Acta Amazonica
repository.name.fl_str_mv Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)
repository.mail.fl_str_mv acta@inpa.gov.br||acta@inpa.gov.br
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