Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Ambiente & Água |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2015000200286 |
Resumo: | The intensive use of pesticides in agriculture has prompted researchers to develop new methods for identifying these pollutants in water. This study sought to validate a high performance liquid chromatography (HPLC) method to determine the concentration of the pesticide glyphosate in groundwater samples by using solid-phase extraction (SPE) filters after derivatization with chloroformate 9-fluorenylmethoxycarbonyl (FMOC-Cl). For the HPLC method, we evaluated the following main validation parameters: linearity, specificity, precision, accuracy, robustness, and limits of detection and quantification. After validation of the method, we determined the concentration of glyphosate in samples from thirteen deep, tubular wells distributed in urban and rural areas in Chapecó, SC, Brazil. The solvent used in the extraction of excess FMOC-Cl was dichloromethane and subsequently filtration was performed on C18 SPE, and injected into the chromatograph column in amino polymer with fluorescence detection. The analytical curve made in ultrapure water was linear, with a correlation coefficient of 0.99. The limits of detection and quantification were 0.24 and 0.07 µg L-1, respectively. Recovery tests in natural waters ranged from 90.37 to 101.70%. Glyphosate was detected in 5 of the thirteen wells evaluated. The highest concentration of glyphosate (6.80 µg L-1) was detected in a countryside well, near the municipal water supply. Despite the low levels of glyphosate detected in our study, any amount present in groundwater samples is worrisome, as these molecules have low ground mobility. |
id |
IPABHI-1_e09ce74f66be1a653504ab60c35e886c |
---|---|
oai_identifier_str |
oai:scielo:S1980-993X2015000200286 |
network_acronym_str |
IPABHI-1 |
network_name_str |
Revista Ambiente & Água |
repository_id_str |
|
spelling |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatizationagriculturechromatographic methodpesticidesThe intensive use of pesticides in agriculture has prompted researchers to develop new methods for identifying these pollutants in water. This study sought to validate a high performance liquid chromatography (HPLC) method to determine the concentration of the pesticide glyphosate in groundwater samples by using solid-phase extraction (SPE) filters after derivatization with chloroformate 9-fluorenylmethoxycarbonyl (FMOC-Cl). For the HPLC method, we evaluated the following main validation parameters: linearity, specificity, precision, accuracy, robustness, and limits of detection and quantification. After validation of the method, we determined the concentration of glyphosate in samples from thirteen deep, tubular wells distributed in urban and rural areas in Chapecó, SC, Brazil. The solvent used in the extraction of excess FMOC-Cl was dichloromethane and subsequently filtration was performed on C18 SPE, and injected into the chromatograph column in amino polymer with fluorescence detection. The analytical curve made in ultrapure water was linear, with a correlation coefficient of 0.99. The limits of detection and quantification were 0.24 and 0.07 µg L-1, respectively. Recovery tests in natural waters ranged from 90.37 to 101.70%. Glyphosate was detected in 5 of the thirteen wells evaluated. The highest concentration of glyphosate (6.80 µg L-1) was detected in a countryside well, near the municipal water supply. Despite the low levels of glyphosate detected in our study, any amount present in groundwater samples is worrisome, as these molecules have low ground mobility.Instituto de Pesquisas Ambientais em Bacias Hidrográficas2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2015000200286Revista Ambiente & Água v.10 n.2 2015reponame:Revista Ambiente & Águainstname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)instacron:IPABHI10.4136/ambi-agua.1548info:eu-repo/semantics/openAccessOlivo,Valdir EduardoTansini,AndréiaCarasek,FábioCordenuzzi,DórysFernandes,SuellenFiori,Marcio AntônioFragoso,AlexMagro,Jacir Daleng2015-05-04T00:00:00Zoai:scielo:S1980-993X2015000200286Revistahttp://www.ambi-agua.net/PUBhttps://old.scielo.br/oai/scielo-oai.php||ambi.agua@gmail.com1980-993X1980-993Xopendoar:2015-05-04T00:00Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)false |
dc.title.none.fl_str_mv |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
title |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
spellingShingle |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization Olivo,Valdir Eduardo agriculture chromatographic method pesticides |
title_short |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
title_full |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
title_fullStr |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
title_full_unstemmed |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
title_sort |
Rapid method for determination of glyphosate in groundwater using high performance liquid chromatography and solid-phase extraction after derivatization |
author |
Olivo,Valdir Eduardo |
author_facet |
Olivo,Valdir Eduardo Tansini,Andréia Carasek,Fábio Cordenuzzi,Dórys Fernandes,Suellen Fiori,Marcio Antônio Fragoso,Alex Magro,Jacir Dal |
author_role |
author |
author2 |
Tansini,Andréia Carasek,Fábio Cordenuzzi,Dórys Fernandes,Suellen Fiori,Marcio Antônio Fragoso,Alex Magro,Jacir Dal |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Olivo,Valdir Eduardo Tansini,Andréia Carasek,Fábio Cordenuzzi,Dórys Fernandes,Suellen Fiori,Marcio Antônio Fragoso,Alex Magro,Jacir Dal |
dc.subject.por.fl_str_mv |
agriculture chromatographic method pesticides |
topic |
agriculture chromatographic method pesticides |
description |
The intensive use of pesticides in agriculture has prompted researchers to develop new methods for identifying these pollutants in water. This study sought to validate a high performance liquid chromatography (HPLC) method to determine the concentration of the pesticide glyphosate in groundwater samples by using solid-phase extraction (SPE) filters after derivatization with chloroformate 9-fluorenylmethoxycarbonyl (FMOC-Cl). For the HPLC method, we evaluated the following main validation parameters: linearity, specificity, precision, accuracy, robustness, and limits of detection and quantification. After validation of the method, we determined the concentration of glyphosate in samples from thirteen deep, tubular wells distributed in urban and rural areas in Chapecó, SC, Brazil. The solvent used in the extraction of excess FMOC-Cl was dichloromethane and subsequently filtration was performed on C18 SPE, and injected into the chromatograph column in amino polymer with fluorescence detection. The analytical curve made in ultrapure water was linear, with a correlation coefficient of 0.99. The limits of detection and quantification were 0.24 and 0.07 µg L-1, respectively. Recovery tests in natural waters ranged from 90.37 to 101.70%. Glyphosate was detected in 5 of the thirteen wells evaluated. The highest concentration of glyphosate (6.80 µg L-1) was detected in a countryside well, near the municipal water supply. Despite the low levels of glyphosate detected in our study, any amount present in groundwater samples is worrisome, as these molecules have low ground mobility. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2015000200286 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2015000200286 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.4136/ambi-agua.1548 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
dc.source.none.fl_str_mv |
Revista Ambiente & Água v.10 n.2 2015 reponame:Revista Ambiente & Água instname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) instacron:IPABHI |
instname_str |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
instacron_str |
IPABHI |
institution |
IPABHI |
reponame_str |
Revista Ambiente & Água |
collection |
Revista Ambiente & Água |
repository.name.fl_str_mv |
Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
repository.mail.fl_str_mv |
||ambi.agua@gmail.com |
_version_ |
1752129749389410304 |