Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use

Detalhes bibliográficos
Autor(a) principal: ZAFALON, ANGELICA T.
Data de Publicação: 2018
Tipo de documento: Tese
Título da fonte: Repositório Institucional do IPEN
Texto Completo: http://repositorio.ipen.br/handle/123456789/29836
Resumo: Hydrogels are natural or synthetic polymer systems that have been vastly applied in the pharmaceutical industry due to their high soft tissue biocompatibility. These hydrogels have been used in dressings as a controlled drug release system. In this study, hydrogels were prepared using poly (N-vinyl-2-pyrrolidone) (PVP), poly (ethylene glycol) (PEG), agar and neomycin followed by gamma irradiation to promote crosslinking and sterilization. The influence of the irradiation process at 25 kGy dose was investigated. The gel fraction and maximum swelling were estimated using physicochemical methods and found about 95% gel fraction and 1100% swelling after 8 hours of immersion. Neomycin released from the hydrogel was measured by the Liquid Chromatography-Mass Spectrometry method and the drug concentration remained constant for 48 hours. Hydrogel / neomycin exhibited antibacterial effect against bacteria and biofilm of Pseudomonas aeruginosa and Staphylococcus aureus. The hydrogel was used in the treatment of pododermatitis in penguins healing process without signs of contamination. With these results, it can be inferred that the hydrogel / neomycin is a suitable candidate for wound dressings.
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spelling Duclerc Fernandes ParraZAFALON, ANGELICA T.20182019-04-04T17:55:42Z2019-04-04T17:55:42Zhttp://repositorio.ipen.br/handle/123456789/2983610.11606/T.85.2020.tde-08112018-113736Hydrogels are natural or synthetic polymer systems that have been vastly applied in the pharmaceutical industry due to their high soft tissue biocompatibility. These hydrogels have been used in dressings as a controlled drug release system. In this study, hydrogels were prepared using poly (N-vinyl-2-pyrrolidone) (PVP), poly (ethylene glycol) (PEG), agar and neomycin followed by gamma irradiation to promote crosslinking and sterilization. The influence of the irradiation process at 25 kGy dose was investigated. The gel fraction and maximum swelling were estimated using physicochemical methods and found about 95% gel fraction and 1100% swelling after 8 hours of immersion. Neomycin released from the hydrogel was measured by the Liquid Chromatography-Mass Spectrometry method and the drug concentration remained constant for 48 hours. Hydrogel / neomycin exhibited antibacterial effect against bacteria and biofilm of Pseudomonas aeruginosa and Staphylococcus aureus. The hydrogel was used in the treatment of pododermatitis in penguins healing process without signs of contamination. With these results, it can be inferred that the hydrogel / neomycin is a suitable candidate for wound dressings.Submitted by Pedro Silva Filho (pfsilva@ipen.br) on 2019-04-04T17:55:42Z No. of bitstreams: 0Made available in DSpace on 2019-04-04T17:55:42Z (GMT). No. of bitstreams: 0Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)Tese (Doutorado em Tecnologia Nuclear)IPEN/TInstituto de Pesquisas Energ??ticas e Nucleares - IPEN-CNEN/SPCAPES: 88881.134341/2016-0195hydrogelssynthesisneomycindrugsantimicrobial agentesdermatitisradiotherapyaquatic organismsbirdsgamma radiationveterinary medicineSynthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary useS??ntese e caracteriza????o de hidrog??is com neomicina para tratamento de infec????es t??picas e uso veterin??rioinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisNS??o Pauloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do IPENinstname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)instacron:IPEN25615ZAFALON, ANGELICA T.19-04https://teses.usp.br/teses/disponiveis/85/85134/tde-08112018-113736/pt-br.php6419ZAFALON, ANGELICA T.:6419:740:SLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ipen.br/bitstream/123456789/29836/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51123456789/298362022-01-11 14:18:42.171oai:repositorio.ipen.br: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Repositório InstitucionalPUBhttp://repositorio.ipen.br/oai/requestbibl@ipen.bropendoar:45102022-01-11T14:18:42Repositório Institucional do IPEN - Instituto de Pesquisas Energéticas e Nucleares (IPEN)false
dc.title.pt_BR.fl_str_mv Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
dc.title.alternative.pt_BR.fl_str_mv S??ntese e caracteriza????o de hidrog??is com neomicina para tratamento de infec????es t??picas e uso veterin??rio
title Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
spellingShingle Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
ZAFALON, ANGELICA T.
hydrogels
synthesis
neomycin
drugs
antimicrobial agentes
dermatitis
radiotherapy
aquatic organisms
birds
gamma radiation
veterinary medicine
title_short Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
title_full Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
title_fullStr Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
title_full_unstemmed Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
title_sort Synthesis and characterization of hydrogels loaded neomycin for infection topic treatment and veterinary use
author ZAFALON, ANGELICA T.
author_facet ZAFALON, ANGELICA T.
author_role author
dc.contributor.advisor1.fl_str_mv Duclerc Fernandes Parra
dc.contributor.author.fl_str_mv ZAFALON, ANGELICA T.
contributor_str_mv Duclerc Fernandes Parra
dc.subject.por.fl_str_mv hydrogels
synthesis
neomycin
drugs
antimicrobial agentes
dermatitis
radiotherapy
aquatic organisms
birds
gamma radiation
veterinary medicine
topic hydrogels
synthesis
neomycin
drugs
antimicrobial agentes
dermatitis
radiotherapy
aquatic organisms
birds
gamma radiation
veterinary medicine
description Hydrogels are natural or synthetic polymer systems that have been vastly applied in the pharmaceutical industry due to their high soft tissue biocompatibility. These hydrogels have been used in dressings as a controlled drug release system. In this study, hydrogels were prepared using poly (N-vinyl-2-pyrrolidone) (PVP), poly (ethylene glycol) (PEG), agar and neomycin followed by gamma irradiation to promote crosslinking and sterilization. The influence of the irradiation process at 25 kGy dose was investigated. The gel fraction and maximum swelling were estimated using physicochemical methods and found about 95% gel fraction and 1100% swelling after 8 hours of immersion. Neomycin released from the hydrogel was measured by the Liquid Chromatography-Mass Spectrometry method and the drug concentration remained constant for 48 hours. Hydrogel / neomycin exhibited antibacterial effect against bacteria and biofilm of Pseudomonas aeruginosa and Staphylococcus aureus. The hydrogel was used in the treatment of pododermatitis in penguins healing process without signs of contamination. With these results, it can be inferred that the hydrogel / neomycin is a suitable candidate for wound dressings.
publishDate 2018
dc.date.pt_BR.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-04-04T17:55:42Z
dc.date.available.fl_str_mv 2019-04-04T17:55:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ipen.br/handle/123456789/29836
dc.identifier.doi.none.fl_str_mv 10.11606/T.85.2020.tde-08112018-113736
url http://repositorio.ipen.br/handle/123456789/29836
identifier_str_mv 10.11606/T.85.2020.tde-08112018-113736
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 95
dc.coverage.pt_BR.fl_str_mv N
dc.source.none.fl_str_mv reponame:Repositório Institucional do IPEN
instname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)
instacron:IPEN
instname_str Instituto de Pesquisas Energéticas e Nucleares (IPEN)
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institution IPEN
reponame_str Repositório Institucional do IPEN
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repository.name.fl_str_mv Repositório Institucional do IPEN - Instituto de Pesquisas Energéticas e Nucleares (IPEN)
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