Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications

Detalhes bibliográficos
Autor(a) principal: Campos, Estefânia V. R.
Data de Publicação: 2021
Outros Autores: Proença, Patricia L. F. [UNESP], Costa, Tais Germano Da, De Lima, Renata, Hedtrich, Sarah, Fraceto, Leonardo Fernandes [UNESP], De Araujo, Daniele Ribeiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1021/acsapm.1c00021
http://hdl.handle.net/11449/233466
Resumo: Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients.
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spelling Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applicationsatopic dermatitisbudesonidepolymeric nanoparticlesthermoresponsive hydrogelstopical releaseAtopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients.Human and Natural Sciences Center Federal University of Abc, Sao PauloDrugs and Bioactives Delivery Systems Research Group - Sislibio Federal University of Abc, Sao PauloDepartment of Environmental Engineering Institute of Science and Technology Sao Paulo State University - Unesp, Sao PauloLaboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials University of Sorocaba (UNISO), Sao PauloFaculty of Pharmaceutical Sciences The University of British ColumbiaDepartment of Environmental Engineering Institute of Science and Technology Sao Paulo State University - Unesp, Sao PauloFederal University of AbcUniversidade Estadual Paulista (UNESP)University of Sorocaba (UNISO)The University of British ColumbiaCampos, Estefânia V. R.Proença, Patricia L. F. [UNESP]Costa, Tais Germano DaDe Lima, RenataHedtrich, SarahFraceto, Leonardo Fernandes [UNESP]De Araujo, Daniele Ribeiro2022-05-01T08:45:00Z2022-05-01T08:45:00Z2021-09-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4436-4449http://dx.doi.org/10.1021/acsapm.1c00021ACS Applied Polymer Materials, v. 3, n. 9, p. 4436-4449, 2021.2637-6105http://hdl.handle.net/11449/23346610.1021/acsapm.1c000212-s2.0-85114052938Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Applied Polymer Materialsinfo:eu-repo/semantics/openAccess2022-05-01T08:45:00Zoai:repositorio.unesp.br:11449/233466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:46:47.002870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
title Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
spellingShingle Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
Campos, Estefânia V. R.
atopic dermatitis
budesonide
polymeric nanoparticles
thermoresponsive hydrogels
topical release
title_short Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
title_full Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
title_fullStr Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
title_full_unstemmed Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
title_sort Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
author Campos, Estefânia V. R.
author_facet Campos, Estefânia V. R.
Proença, Patricia L. F. [UNESP]
Costa, Tais Germano Da
De Lima, Renata
Hedtrich, Sarah
Fraceto, Leonardo Fernandes [UNESP]
De Araujo, Daniele Ribeiro
author_role author
author2 Proença, Patricia L. F. [UNESP]
Costa, Tais Germano Da
De Lima, Renata
Hedtrich, Sarah
Fraceto, Leonardo Fernandes [UNESP]
De Araujo, Daniele Ribeiro
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Abc
Universidade Estadual Paulista (UNESP)
University of Sorocaba (UNISO)
The University of British Columbia
dc.contributor.author.fl_str_mv Campos, Estefânia V. R.
Proença, Patricia L. F. [UNESP]
Costa, Tais Germano Da
De Lima, Renata
Hedtrich, Sarah
Fraceto, Leonardo Fernandes [UNESP]
De Araujo, Daniele Ribeiro
dc.subject.por.fl_str_mv atopic dermatitis
budesonide
polymeric nanoparticles
thermoresponsive hydrogels
topical release
topic atopic dermatitis
budesonide
polymeric nanoparticles
thermoresponsive hydrogels
topical release
description Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-10
2022-05-01T08:45:00Z
2022-05-01T08:45:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1021/acsapm.1c00021
ACS Applied Polymer Materials, v. 3, n. 9, p. 4436-4449, 2021.
2637-6105
http://hdl.handle.net/11449/233466
10.1021/acsapm.1c00021
2-s2.0-85114052938
url http://dx.doi.org/10.1021/acsapm.1c00021
http://hdl.handle.net/11449/233466
identifier_str_mv ACS Applied Polymer Materials, v. 3, n. 9, p. 4436-4449, 2021.
2637-6105
10.1021/acsapm.1c00021
2-s2.0-85114052938
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ACS Applied Polymer Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4436-4449
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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