Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acsapm.1c00021 http://hdl.handle.net/11449/233466 |
Resumo: | Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients. |
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Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applicationsatopic dermatitisbudesonidepolymeric nanoparticlesthermoresponsive hydrogelstopical releaseAtopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients.Human and Natural Sciences Center Federal University of Abc, Sao PauloDrugs and Bioactives Delivery Systems Research Group - Sislibio Federal University of Abc, Sao PauloDepartment of Environmental Engineering Institute of Science and Technology Sao Paulo State University - Unesp, Sao PauloLaboratory for Evaluation of the Bioactivity and Toxicology of Nanomaterials University of Sorocaba (UNISO), Sao PauloFaculty of Pharmaceutical Sciences The University of British ColumbiaDepartment of Environmental Engineering Institute of Science and Technology Sao Paulo State University - Unesp, Sao PauloFederal University of AbcUniversidade Estadual Paulista (UNESP)University of Sorocaba (UNISO)The University of British ColumbiaCampos, Estefânia V. R.Proença, Patricia L. F. [UNESP]Costa, Tais Germano DaDe Lima, RenataHedtrich, SarahFraceto, Leonardo Fernandes [UNESP]De Araujo, Daniele Ribeiro2022-05-01T08:45:00Z2022-05-01T08:45:00Z2021-09-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4436-4449http://dx.doi.org/10.1021/acsapm.1c00021ACS Applied Polymer Materials, v. 3, n. 9, p. 4436-4449, 2021.2637-6105http://hdl.handle.net/11449/23346610.1021/acsapm.1c000212-s2.0-85114052938Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Applied Polymer Materialsinfo:eu-repo/semantics/openAccess2022-05-01T08:45:00Zoai:repositorio.unesp.br:11449/233466Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:46:47.002870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
title |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
spellingShingle |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications Campos, Estefânia V. R. atopic dermatitis budesonide polymeric nanoparticles thermoresponsive hydrogels topical release |
title_short |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
title_full |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
title_fullStr |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
title_full_unstemmed |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
title_sort |
Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications |
author |
Campos, Estefânia V. R. |
author_facet |
Campos, Estefânia V. R. Proença, Patricia L. F. [UNESP] Costa, Tais Germano Da De Lima, Renata Hedtrich, Sarah Fraceto, Leonardo Fernandes [UNESP] De Araujo, Daniele Ribeiro |
author_role |
author |
author2 |
Proença, Patricia L. F. [UNESP] Costa, Tais Germano Da De Lima, Renata Hedtrich, Sarah Fraceto, Leonardo Fernandes [UNESP] De Araujo, Daniele Ribeiro |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Abc Universidade Estadual Paulista (UNESP) University of Sorocaba (UNISO) The University of British Columbia |
dc.contributor.author.fl_str_mv |
Campos, Estefânia V. R. Proença, Patricia L. F. [UNESP] Costa, Tais Germano Da De Lima, Renata Hedtrich, Sarah Fraceto, Leonardo Fernandes [UNESP] De Araujo, Daniele Ribeiro |
dc.subject.por.fl_str_mv |
atopic dermatitis budesonide polymeric nanoparticles thermoresponsive hydrogels topical release |
topic |
atopic dermatitis budesonide polymeric nanoparticles thermoresponsive hydrogels topical release |
description |
Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disorder characterized by intense itching and recurrent eczematous lesions. Topical corticosteroids are the first-line treatment to control moderate-to-severe AD; however, prolonged application of corticosteroids is required, which can result in dermal atrophy as a side effect. Drug-delivery systems can provide more effective and targeted therapy strategies. In this study, budesonide (BUD) was encapsulated into chitosan (CS)-coated PLGA nanoparticles, which were further incorporated into poloxamer hydrogels to improve the anti-inflammatory activity and decrease adverse effects. The nanoparticles were prepared by the emulsification-solvent evaporation technique, and their physicochemical characteristics were evaluated. Rheological properties of the hydrogels, such as viscosity and sol-gel transition temperature, were evaluated with and without nanoparticles. In vitro release kinetics and ex vivo drug absorption studies were performed using Franz diffusion cells. The nanoparticles showed a mean diameter of 324 ± 4 nm, positive ζ potential (20 mV) due to CS coating, and high encapsulation efficiency (>90%). The nanoparticles did not show cytotoxic effects in primary human fibroblasts and keratinocytes; however, all formulations induced the generation of reactive oxygen species. Both nanoparticles and hydrogels were able to change the release kinetics of BUD when compared to the nonencapsulated compound. Nanoparticles were not able to surmount the stratum corneum of excised human skin, but the nanoencapsulation facilitated the skin absorption of BUD. The hydrogels containing nanoparticles or not showed non-Newtonian and pseudoplastic behavior. The nanoformulations seem to be a good candidate to deliver glucocorticoids in the skin of AD patients. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-10 2022-05-01T08:45:00Z 2022-05-01T08:45:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acsapm.1c00021 ACS Applied Polymer Materials, v. 3, n. 9, p. 4436-4449, 2021. 2637-6105 http://hdl.handle.net/11449/233466 10.1021/acsapm.1c00021 2-s2.0-85114052938 |
url |
http://dx.doi.org/10.1021/acsapm.1c00021 http://hdl.handle.net/11449/233466 |
identifier_str_mv |
ACS Applied Polymer Materials, v. 3, n. 9, p. 4436-4449, 2021. 2637-6105 10.1021/acsapm.1c00021 2-s2.0-85114052938 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ACS Applied Polymer Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4436-4449 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129356365561856 |