Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo de conferência |
Título da fonte: | Repositório Institucional do IPEN |
Texto Completo: | http://repositorio.ipen.br/handle/123456789/29107 |
Resumo: | An endotracheal tube (ETT) is required for the management of critically ill, mechanically ventilated patients. Ventilatorassociated pneumonia (VAP) affects patients hospitalized in intensive care units; its risk of occurrence is 1% to up 3% for each day of mechanical ventilation. The polymicrobial nature of VAP is established with mixed bacterial-fungal biofilms colonizing the ETT. The microbial interaction enhances the microbial pathogenesis contributing to high indexes of morbidity/mortality. Antimicrobial Photodynamic Therapy (aPDT) could be a suitable therapy for decontamination of oral cavity and ETT at the same time, but the use of a fiber optics inside the ETT seems to not be appropriated since a cannula for secretion aspiration has to be introduced into the ETT to keep it??s lumen. The aim of this study is to proof the concept that an external light source from a LED is capable of reach all areas of the ETT. We use a commercial ETT, 60??M methylene blue (MB), and a 660nm diode laser and calculated the transmission coefficient of light in different situations as only tube, tube with biofilm and biofilm+MB. The results prove that is possible to transmit light through the tube even in the presence of MB and biofilm although a high attenuation of about 60% was measured depending on the tested condition. |
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DAI, TIANHONGBASSI, ROSANEMYAKAWA, WALTERNAVARRO, RICARDO S.BAPTISTA, ALESSANDRARIBEIRO, MARTHA S.NUNEZ, SILVIA C.LIGHT-BASED DIAGNOSIS AND TREATMENT OF INFECTIOUS DISEASES2018-09-12T13:33:55Z2018-09-12T13:33:55ZJanuary 27 - February 01, 2018http://repositorio.ipen.br/handle/123456789/2910710.1117/12.2290764An endotracheal tube (ETT) is required for the management of critically ill, mechanically ventilated patients. Ventilatorassociated pneumonia (VAP) affects patients hospitalized in intensive care units; its risk of occurrence is 1% to up 3% for each day of mechanical ventilation. The polymicrobial nature of VAP is established with mixed bacterial-fungal biofilms colonizing the ETT. The microbial interaction enhances the microbial pathogenesis contributing to high indexes of morbidity/mortality. Antimicrobial Photodynamic Therapy (aPDT) could be a suitable therapy for decontamination of oral cavity and ETT at the same time, but the use of a fiber optics inside the ETT seems to not be appropriated since a cannula for secretion aspiration has to be introduced into the ETT to keep it??s lumen. The aim of this study is to proof the concept that an external light source from a LED is capable of reach all areas of the ETT. We use a commercial ETT, 60??M methylene blue (MB), and a 660nm diode laser and calculated the transmission coefficient of light in different situations as only tube, tube with biofilm and biofilm+MB. The results prove that is possible to transmit light through the tube even in the presence of MB and biofilm although a high attenuation of about 60% was measured depending on the tested condition.Submitted by Marco Antonio Oliveira da Silva (maosilva@ipen.br) on 2018-09-12T13:33:55Z No. of bitstreams: 1 24929.pdf: 1252941 bytes, checksum: 2fc654fec332bac2266573bc728d6309 (MD5)Made available in DSpace on 2018-09-12T13:33:55Z (GMT). No. of bitstreams: 1 24929.pdf: 1252941 bytes, checksum: 2fc654fec332bac2266573bc728d6309 (MD5)1047917-1 - 1047917-5Society of Photo-optical Instrumentation EngineersSPIE Proceedings Series, 10479Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation sourceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSPIEIBellingham, WA, USASan Francisco, California, USAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do IPENinstname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)instacron:IPEN249292018RIBEIRO, MARTHA S.18-09Proceedings574RIBEIRO, MARTHA S.:574:920:NORIGINAL24929.pdf24929.pdfapplication/pdf1252941http://repositorio.ipen.br/bitstream/123456789/29107/1/24929.pdf2fc654fec332bac2266573bc728d6309MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ipen.br/bitstream/123456789/29107/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/291072018-09-12 13:33:55.61oai:repositorio.ipen.br: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Repositório InstitucionalPUBhttp://repositorio.ipen.br/oai/requestbibl@ipen.bropendoar:45102018-09-12T13:33:55Repositório Institucional do IPEN - Instituto de Pesquisas Energéticas e Nucleares (IPEN)false |
dc.title.pt_BR.fl_str_mv |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
title |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
spellingShingle |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source BASSI, ROSANE |
title_short |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
title_full |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
title_fullStr |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
title_full_unstemmed |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
title_sort |
Photodynamic Therapy to destroy pneumonia associated microorganisms using external irradiation source |
author |
BASSI, ROSANE |
author_facet |
BASSI, ROSANE MYAKAWA, WALTER NAVARRO, RICARDO S. BAPTISTA, ALESSANDRA RIBEIRO, MARTHA S. NUNEZ, SILVIA C. LIGHT-BASED DIAGNOSIS AND TREATMENT OF INFECTIOUS DISEASES |
author_role |
author |
author2 |
MYAKAWA, WALTER NAVARRO, RICARDO S. BAPTISTA, ALESSANDRA RIBEIRO, MARTHA S. NUNEZ, SILVIA C. LIGHT-BASED DIAGNOSIS AND TREATMENT OF INFECTIOUS DISEASES |
author2_role |
author author author author author author |
dc.contributor.editor.none.fl_str_mv |
DAI, TIANHONG |
dc.contributor.author.fl_str_mv |
BASSI, ROSANE MYAKAWA, WALTER NAVARRO, RICARDO S. BAPTISTA, ALESSANDRA RIBEIRO, MARTHA S. NUNEZ, SILVIA C. LIGHT-BASED DIAGNOSIS AND TREATMENT OF INFECTIOUS DISEASES |
description |
An endotracheal tube (ETT) is required for the management of critically ill, mechanically ventilated patients. Ventilatorassociated pneumonia (VAP) affects patients hospitalized in intensive care units; its risk of occurrence is 1% to up 3% for each day of mechanical ventilation. The polymicrobial nature of VAP is established with mixed bacterial-fungal biofilms colonizing the ETT. The microbial interaction enhances the microbial pathogenesis contributing to high indexes of morbidity/mortality. Antimicrobial Photodynamic Therapy (aPDT) could be a suitable therapy for decontamination of oral cavity and ETT at the same time, but the use of a fiber optics inside the ETT seems to not be appropriated since a cannula for secretion aspiration has to be introduced into the ETT to keep it??s lumen. The aim of this study is to proof the concept that an external light source from a LED is capable of reach all areas of the ETT. We use a commercial ETT, 60??M methylene blue (MB), and a 660nm diode laser and calculated the transmission coefficient of light in different situations as only tube, tube with biofilm and biofilm+MB. The results prove that is possible to transmit light through the tube even in the presence of MB and biofilm although a high attenuation of about 60% was measured depending on the tested condition. |
publishDate |
2018 |
dc.date.evento.pt_BR.fl_str_mv |
January 27 - February 01, 2018 |
dc.date.accessioned.fl_str_mv |
2018-09-12T13:33:55Z |
dc.date.available.fl_str_mv |
2018-09-12T13:33:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/conferenceObject |
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conferenceObject |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ipen.br/handle/123456789/29107 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1117/12.2290764 |
url |
http://repositorio.ipen.br/handle/123456789/29107 |
identifier_str_mv |
10.1117/12.2290764 |
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SPIE Proceedings Series, 10479 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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1047917-1 - 1047917-5 |
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I |
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Society of Photo-optical Instrumentation Engineers |
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Society of Photo-optical Instrumentation Engineers |
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