Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra

Detalhes bibliográficos
Autor(a) principal: CASTRO, PEDRO A.A. de
Data de Publicação: 2018
Outros Autores: DIAS, DERLY A., ZEZELL, DENISE M., INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
Tipo de documento: Artigo de conferência
Título da fonte: Repositório Institucional do IPEN
Texto Completo: http://repositorio.ipen.br/handle/123456789/28278
Resumo: Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses.
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spelling 2018-01-11T10:19:14Z2018-01-11T10:19:14ZOctober 22-27, 2017http://repositorio.ipen.br/handle/123456789/28278Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses.Submitted by Marco Antonio Oliveira da Silva (maosilva@ipen.br) on 2018-01-11T10:19:14Z No. of bitstreams: 1 24103.pdf: 578741 bytes, checksum: 552e2a53616324a718d87ae35c7a5433 (MD5)Made available in DSpace on 2018-01-11T10:19:14Z (GMT). No. of bitstreams: 1 24103.pdf: 578741 bytes, checksum: 552e2a53616324a718d87ae35c7a5433 (MD5)Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)CNPq: 465763/2014-6; PQ 312397/2013-5CAPES: 88881.068505/2014-01Associa????o Brasileira de Energia Nuclearcattlecobalt 60femurfourier transformationinfrared spectraionizing radiationsmultivariate analysisradiation dose rangesradiation dosesradiation effectsCharacterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectINACIRio de Janeiro, RJBelo Horizonte, MGCASTRO, PEDRO A.A. deDIAS, DERLY A.ZEZELL, DENISE M.INTERNATIONAL NUCLEAR ATLANTIC CONFERENCEinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do IPENinstname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)instacron:IPEN241032017CASTRO, PEDRO A.A. deDIAS, DERLY A.ZEZELL, DENISE M.18-01Proceedings1205312688693CASTRO, PEDRO A.A. 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dc.title.pt_BR.fl_str_mv Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
title Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
spellingShingle Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
CASTRO, PEDRO A.A. de
cattle
cobalt 60
femur
fourier transformation
infrared spectra
ionizing radiations
multivariate analysis
radiation dose ranges
radiation doses
radiation effects
title_short Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
title_full Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
title_fullStr Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
title_full_unstemmed Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
title_sort Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
author CASTRO, PEDRO A.A. de
author_facet CASTRO, PEDRO A.A. de
DIAS, DERLY A.
ZEZELL, DENISE M.
INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
author_role author
author2 DIAS, DERLY A.
ZEZELL, DENISE M.
INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
author2_role author
author
author
dc.contributor.author.fl_str_mv CASTRO, PEDRO A.A. de
DIAS, DERLY A.
ZEZELL, DENISE M.
INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE
dc.subject.por.fl_str_mv cattle
cobalt 60
femur
fourier transformation
infrared spectra
ionizing radiations
multivariate analysis
radiation dose ranges
radiation doses
radiation effects
topic cattle
cobalt 60
femur
fourier transformation
infrared spectra
ionizing radiations
multivariate analysis
radiation dose ranges
radiation doses
radiation effects
description Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses.
publishDate 2018
dc.date.evento.pt_BR.fl_str_mv October 22-27, 2017
dc.date.accessioned.fl_str_mv 2018-01-11T10:19:14Z
dc.date.available.fl_str_mv 2018-01-11T10:19:14Z
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dc.identifier.uri.fl_str_mv http://repositorio.ipen.br/handle/123456789/28278
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dc.publisher.none.fl_str_mv Associa????o Brasileira de Energia Nuclear
publisher.none.fl_str_mv Associa????o Brasileira de Energia Nuclear
dc.source.none.fl_str_mv reponame:Repositório Institucional do IPEN
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