Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo de conferência |
Título da fonte: | Repositório Institucional do IPEN |
Texto Completo: | http://repositorio.ipen.br/handle/123456789/28278 |
Resumo: | Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses. |
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2018-01-11T10:19:14Z2018-01-11T10:19:14ZOctober 22-27, 2017http://repositorio.ipen.br/handle/123456789/28278Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses.Submitted by Marco Antonio Oliveira da Silva (maosilva@ipen.br) on 2018-01-11T10:19:14Z No. of bitstreams: 1 24103.pdf: 578741 bytes, checksum: 552e2a53616324a718d87ae35c7a5433 (MD5)Made available in DSpace on 2018-01-11T10:19:14Z (GMT). No. of bitstreams: 1 24103.pdf: 578741 bytes, checksum: 552e2a53616324a718d87ae35c7a5433 (MD5)Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)CNPq: 465763/2014-6; PQ 312397/2013-5CAPES: 88881.068505/2014-01Associa????o Brasileira de Energia Nuclearcattlecobalt 60femurfourier transformationinfrared spectraionizing radiationsmultivariate analysisradiation dose rangesradiation dosesradiation effectsCharacterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectrainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectINACIRio de Janeiro, RJBelo Horizonte, MGCASTRO, PEDRO A.A. deDIAS, DERLY A.ZEZELL, DENISE M.INTERNATIONAL NUCLEAR ATLANTIC CONFERENCEinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do IPENinstname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)instacron:IPEN241032017CASTRO, PEDRO A.A. deDIAS, DERLY A.ZEZELL, DENISE M.18-01Proceedings1205312688693CASTRO, PEDRO A.A. DE:12053:930:SDIAS, DERLY A.:12688:930:NZEZELL, DENISE M.:693:920:NORIGINAL24103.pdf24103.pdfapplication/pdf578741http://repositorio.ipen.br/bitstream/123456789/28278/1/24103.pdf552e2a53616324a718d87ae35c7a5433MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ipen.br/bitstream/123456789/28278/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/282782022-03-24 16:43:22.864oai:repositorio.ipen.br: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Repositório InstitucionalPUBhttp://repositorio.ipen.br/oai/requestbibl@ipen.bropendoar:45102022-03-24T16:43:22Repositório Institucional do IPEN - Instituto de Pesquisas Energéticas e Nucleares (IPEN)false |
dc.title.pt_BR.fl_str_mv |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
title |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
spellingShingle |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra CASTRO, PEDRO A.A. de cattle cobalt 60 femur fourier transformation infrared spectra ionizing radiations multivariate analysis radiation dose ranges radiation doses radiation effects |
title_short |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
title_full |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
title_fullStr |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
title_full_unstemmed |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
title_sort |
Characterization of ionizing radiation effects on bone using Fourier Transform Infrared Spectroscopy and multivariate analysis of spectra |
author |
CASTRO, PEDRO A.A. de |
author_facet |
CASTRO, PEDRO A.A. de DIAS, DERLY A. ZEZELL, DENISE M. INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE |
author_role |
author |
author2 |
DIAS, DERLY A. ZEZELL, DENISE M. INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
CASTRO, PEDRO A.A. de DIAS, DERLY A. ZEZELL, DENISE M. INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE |
dc.subject.por.fl_str_mv |
cattle cobalt 60 femur fourier transformation infrared spectra ionizing radiations multivariate analysis radiation dose ranges radiation doses radiation effects |
topic |
cattle cobalt 60 femur fourier transformation infrared spectra ionizing radiations multivariate analysis radiation dose ranges radiation doses radiation effects |
description |
Ionizing radiation has been used as an important treatment and diagnostic method for several diseases. Optical techniques provides an efficient clinical diagnostic to support an accurate evaluation of the interaction of radiation with molecules. Fourier-transform infrared spectroscopy coupled with attenuated total reflectance (ATR-FTIR) is a label-free and nondestructive optical technique that can recognize functional groups in biological samples. In this work, 30 fragments of bone were collected from bovine femur diaphysis. Samples were cut and polished until 1 cm x 1 cm x 1 mm, which were then stored properly in the refrigerated environment. Samples irradiation was performed with a Cobalt-60 Gammacell Irradiator source at doses of 0.1 kGy, 1 kGy, whereas the fragments exposed to dose of 15 kGy was irradiated in a multipurpose irradiator of Cobalt-60. Spectral data was submitted to principal component analysis followed by linear discriminant analysis. Multivariate analysis was performed with Principal component analysis(PCA) followed by Linear Discriminant Analysis(LDA) using MATLAB R2015a software (The Mathworks Inc., Natick, MA, USA). We demonstrated the feasibility of using ATR-FTIR spectroscopy associated with PCA-LDA multivariate technique to evaluate the molecular changes in bone matrix caused by different doses: 0.1 kGy, 1 kGy and 15 kGy. These alterations between the groups are mainly reported in phosphate region. Our results open up new possibilities for protein monitoring relating to dose responses. |
publishDate |
2018 |
dc.date.evento.pt_BR.fl_str_mv |
October 22-27, 2017 |
dc.date.accessioned.fl_str_mv |
2018-01-11T10:19:14Z |
dc.date.available.fl_str_mv |
2018-01-11T10:19:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
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conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ipen.br/handle/123456789/28278 |
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http://repositorio.ipen.br/handle/123456789/28278 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.coverage.pt_BR.fl_str_mv |
I |
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Associa????o Brasileira de Energia Nuclear |
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Associa????o Brasileira de Energia Nuclear |
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