Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls

Detalhes bibliográficos
Autor(a) principal: Oliveira,Joazir Rodrigues
Data de Publicação: 2022
Outros Autores: Pedroso,Rafaela Cássia da Cunha, Cunha,Sirbene Nunes da, Castro,Vinicius Silva, Cunha Neto,Adelino da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Food Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232022000100401
Resumo: Abstract This study aimed to investigate the occurrence of parasitic structures in curly lettuce (Lactuca sativa L.) sold in food stalls open markets in Cuiabá city, in the Brazilian state of Mato Grosso (MT). For this purpose, 50 samples were evaluated using spontaneous sedimentation and centrifugal flotation methods. These techniques showed positivity in 96% (48/50) and 78% (39/50) of the samples, respectively. The sedimentation method was statistically (p ≤ 0.05) more effective in detecting parasitic structures of the pathogens as Entamoeba histolytica, Ascaris spp., Strongyloides spp., Larvae and Hookworm eggs, when comparing both methods. However, these methods did not differ significantly for the detection of Giardia lamblia. Ascaris spp and Entamoeba coli occurred in 64% (32/50) and 46% (23/50) of the positive samples, respectively, with the highest percentages observed. The occurrence of Blastocystis spp., Fasciola spp., Dipylidium caninum and Ascaris lumbricoides revealed detection exclusively by the spontaneous sedimentation method, while Iodamoeba butschlii and Enterobius vermicularis were detected by centrifugal– flotation procedure. These results could reinforce the need to use methods based on sedimentation and the fluctuation of parasite structures in the analysis of lettuce. The presence of parasites in lettuce by fecal origin, with recognized pathogenicity, represents a risk to consumer health and demonstrates a deficit in good agricultural practices in the production, distribution and commercialization of lettuce.
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spelling Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stallsLactuca sativaProtozoaHelminthsAbstract This study aimed to investigate the occurrence of parasitic structures in curly lettuce (Lactuca sativa L.) sold in food stalls open markets in Cuiabá city, in the Brazilian state of Mato Grosso (MT). For this purpose, 50 samples were evaluated using spontaneous sedimentation and centrifugal flotation methods. These techniques showed positivity in 96% (48/50) and 78% (39/50) of the samples, respectively. The sedimentation method was statistically (p ≤ 0.05) more effective in detecting parasitic structures of the pathogens as Entamoeba histolytica, Ascaris spp., Strongyloides spp., Larvae and Hookworm eggs, when comparing both methods. However, these methods did not differ significantly for the detection of Giardia lamblia. Ascaris spp and Entamoeba coli occurred in 64% (32/50) and 46% (23/50) of the positive samples, respectively, with the highest percentages observed. The occurrence of Blastocystis spp., Fasciola spp., Dipylidium caninum and Ascaris lumbricoides revealed detection exclusively by the spontaneous sedimentation method, while Iodamoeba butschlii and Enterobius vermicularis were detected by centrifugal– flotation procedure. These results could reinforce the need to use methods based on sedimentation and the fluctuation of parasite structures in the analysis of lettuce. The presence of parasites in lettuce by fecal origin, with recognized pathogenicity, represents a risk to consumer health and demonstrates a deficit in good agricultural practices in the production, distribution and commercialization of lettuce.Instituto de Tecnologia de Alimentos - ITAL2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232022000100401Brazilian Journal of Food Technology v.25 2022reponame:Brazilian Journal of Food Technologyinstname:Instituto de Tecnologia de Alimentos (ITAL)instacron:ITAL10.1590/1981-6723.00221info:eu-repo/semantics/openAccessOliveira,Joazir RodriguesPedroso,Rafaela Cássia da CunhaCunha,Sirbene Nunes daCastro,Vinicius SilvaCunha Neto,Adelino daeng2022-02-11T00:00:00Zoai:scielo:S1981-67232022000100401Revistahttp://bjft.ital.sp.gov.br/https://old.scielo.br/oai/scielo-oai.phpbjftsec@ital.sp.gov.br||bjftsec@ital.sp.gov.br1981-67231516-7275opendoar:2022-02-11T00:00Brazilian Journal of Food Technology - Instituto de Tecnologia de Alimentos (ITAL)false
dc.title.none.fl_str_mv Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
title Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
spellingShingle Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
Oliveira,Joazir Rodrigues
Lactuca sativa
Protozoa
Helminths
title_short Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
title_full Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
title_fullStr Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
title_full_unstemmed Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
title_sort Evaluation of two analytical methods of detection for intestinal parasites in curly lettuce sold in food stalls
author Oliveira,Joazir Rodrigues
author_facet Oliveira,Joazir Rodrigues
Pedroso,Rafaela Cássia da Cunha
Cunha,Sirbene Nunes da
Castro,Vinicius Silva
Cunha Neto,Adelino da
author_role author
author2 Pedroso,Rafaela Cássia da Cunha
Cunha,Sirbene Nunes da
Castro,Vinicius Silva
Cunha Neto,Adelino da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Joazir Rodrigues
Pedroso,Rafaela Cássia da Cunha
Cunha,Sirbene Nunes da
Castro,Vinicius Silva
Cunha Neto,Adelino da
dc.subject.por.fl_str_mv Lactuca sativa
Protozoa
Helminths
topic Lactuca sativa
Protozoa
Helminths
description Abstract This study aimed to investigate the occurrence of parasitic structures in curly lettuce (Lactuca sativa L.) sold in food stalls open markets in Cuiabá city, in the Brazilian state of Mato Grosso (MT). For this purpose, 50 samples were evaluated using spontaneous sedimentation and centrifugal flotation methods. These techniques showed positivity in 96% (48/50) and 78% (39/50) of the samples, respectively. The sedimentation method was statistically (p ≤ 0.05) more effective in detecting parasitic structures of the pathogens as Entamoeba histolytica, Ascaris spp., Strongyloides spp., Larvae and Hookworm eggs, when comparing both methods. However, these methods did not differ significantly for the detection of Giardia lamblia. Ascaris spp and Entamoeba coli occurred in 64% (32/50) and 46% (23/50) of the positive samples, respectively, with the highest percentages observed. The occurrence of Blastocystis spp., Fasciola spp., Dipylidium caninum and Ascaris lumbricoides revealed detection exclusively by the spontaneous sedimentation method, while Iodamoeba butschlii and Enterobius vermicularis were detected by centrifugal– flotation procedure. These results could reinforce the need to use methods based on sedimentation and the fluctuation of parasite structures in the analysis of lettuce. The presence of parasites in lettuce by fecal origin, with recognized pathogenicity, represents a risk to consumer health and demonstrates a deficit in good agricultural practices in the production, distribution and commercialization of lettuce.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232022000100401
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232022000100401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1981-6723.00221
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia de Alimentos - ITAL
publisher.none.fl_str_mv Instituto de Tecnologia de Alimentos - ITAL
dc.source.none.fl_str_mv Brazilian Journal of Food Technology v.25 2022
reponame:Brazilian Journal of Food Technology
instname:Instituto de Tecnologia de Alimentos (ITAL)
instacron:ITAL
instname_str Instituto de Tecnologia de Alimentos (ITAL)
instacron_str ITAL
institution ITAL
reponame_str Brazilian Journal of Food Technology
collection Brazilian Journal of Food Technology
repository.name.fl_str_mv Brazilian Journal of Food Technology - Instituto de Tecnologia de Alimentos (ITAL)
repository.mail.fl_str_mv bjftsec@ital.sp.gov.br||bjftsec@ital.sp.gov.br
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