Comparison of mould filament counting techniques for industrialized tomato sauces
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Food Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100446 |
Resumo: | Abstract The objective of this study was to compare different techniques for counting mould filaments in industrialized tomato sauces available on the Brazilian market. In order to do so, 42 samples of the product “traditional tomato sauce” (from 21 different brands), sold in supermarkets in the municipality of the city of Bauru, Brazil, were collected during the period from April to June, 2016. The mycelial moulds were counted by the Howard method according to the official methodology recommended by the Association of Official Analytical Chemists (AOAC), applying techniques 965.41 and 945.92. Notably, the application of technique 945.92 using sodium hydroxide (NaOH) to eliminate the starch in the samples, facilitated counting of the hyphae and, consequently, the percentages of positive fields observed were significantly higher (median = 10%) when compared with the percentages obtained after using technique 965.41 (median = 6%) (p < 0.001). The present findings reveal the need to expand the discussion concerning the application of different techniques for the counting of mould filaments in industrialized tomato sauces, especially in Brazil and in other countries that do not prohibit the addition of starch to this kind of product. |
id |
ITAL-1_a89a3a3818d306034a32e2c974a6798a |
---|---|
oai_identifier_str |
oai:scielo:S1981-67232018000100446 |
network_acronym_str |
ITAL-1 |
network_name_str |
Brazilian Journal of Food Technology |
repository_id_str |
|
spelling |
Comparison of mould filament counting techniques for industrialized tomato saucesTomatoTomato sauceHoward methodMould filamentsMicroscopic analysisContaminationAbstract The objective of this study was to compare different techniques for counting mould filaments in industrialized tomato sauces available on the Brazilian market. In order to do so, 42 samples of the product “traditional tomato sauce” (from 21 different brands), sold in supermarkets in the municipality of the city of Bauru, Brazil, were collected during the period from April to June, 2016. The mycelial moulds were counted by the Howard method according to the official methodology recommended by the Association of Official Analytical Chemists (AOAC), applying techniques 965.41 and 945.92. Notably, the application of technique 945.92 using sodium hydroxide (NaOH) to eliminate the starch in the samples, facilitated counting of the hyphae and, consequently, the percentages of positive fields observed were significantly higher (median = 10%) when compared with the percentages obtained after using technique 965.41 (median = 6%) (p < 0.001). The present findings reveal the need to expand the discussion concerning the application of different techniques for the counting of mould filaments in industrialized tomato sauces, especially in Brazil and in other countries that do not prohibit the addition of starch to this kind of product.Instituto de Tecnologia de Alimentos - ITAL2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100446Brazilian Journal of Food Technology v.21 2018reponame:Brazilian Journal of Food Technologyinstname:Instituto de Tecnologia de Alimentos (ITAL)instacron:ITAL10.1590/1981-6723.23417info:eu-repo/semantics/openAccessStancari,Regina Célia ArantesPauli,Larissa Fiamengui deNascentes,Gabriel Antonio NogueiraAnversa,Laíseng2018-06-05T00:00:00Zoai:scielo:S1981-67232018000100446Revistahttp://bjft.ital.sp.gov.br/https://old.scielo.br/oai/scielo-oai.phpbjftsec@ital.sp.gov.br||bjftsec@ital.sp.gov.br1981-67231516-7275opendoar:2018-06-05T00:00Brazilian Journal of Food Technology - Instituto de Tecnologia de Alimentos (ITAL)false |
dc.title.none.fl_str_mv |
Comparison of mould filament counting techniques for industrialized tomato sauces |
title |
Comparison of mould filament counting techniques for industrialized tomato sauces |
spellingShingle |
Comparison of mould filament counting techniques for industrialized tomato sauces Stancari,Regina Célia Arantes Tomato Tomato sauce Howard method Mould filaments Microscopic analysis Contamination |
title_short |
Comparison of mould filament counting techniques for industrialized tomato sauces |
title_full |
Comparison of mould filament counting techniques for industrialized tomato sauces |
title_fullStr |
Comparison of mould filament counting techniques for industrialized tomato sauces |
title_full_unstemmed |
Comparison of mould filament counting techniques for industrialized tomato sauces |
title_sort |
Comparison of mould filament counting techniques for industrialized tomato sauces |
author |
Stancari,Regina Célia Arantes |
author_facet |
Stancari,Regina Célia Arantes Pauli,Larissa Fiamengui de Nascentes,Gabriel Antonio Nogueira Anversa,Laís |
author_role |
author |
author2 |
Pauli,Larissa Fiamengui de Nascentes,Gabriel Antonio Nogueira Anversa,Laís |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Stancari,Regina Célia Arantes Pauli,Larissa Fiamengui de Nascentes,Gabriel Antonio Nogueira Anversa,Laís |
dc.subject.por.fl_str_mv |
Tomato Tomato sauce Howard method Mould filaments Microscopic analysis Contamination |
topic |
Tomato Tomato sauce Howard method Mould filaments Microscopic analysis Contamination |
description |
Abstract The objective of this study was to compare different techniques for counting mould filaments in industrialized tomato sauces available on the Brazilian market. In order to do so, 42 samples of the product “traditional tomato sauce” (from 21 different brands), sold in supermarkets in the municipality of the city of Bauru, Brazil, were collected during the period from April to June, 2016. The mycelial moulds were counted by the Howard method according to the official methodology recommended by the Association of Official Analytical Chemists (AOAC), applying techniques 965.41 and 945.92. Notably, the application of technique 945.92 using sodium hydroxide (NaOH) to eliminate the starch in the samples, facilitated counting of the hyphae and, consequently, the percentages of positive fields observed were significantly higher (median = 10%) when compared with the percentages obtained after using technique 965.41 (median = 6%) (p < 0.001). The present findings reveal the need to expand the discussion concerning the application of different techniques for the counting of mould filaments in industrialized tomato sauces, especially in Brazil and in other countries that do not prohibit the addition of starch to this kind of product. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100446 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100446 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1981-6723.23417 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia de Alimentos - ITAL |
publisher.none.fl_str_mv |
Instituto de Tecnologia de Alimentos - ITAL |
dc.source.none.fl_str_mv |
Brazilian Journal of Food Technology v.21 2018 reponame:Brazilian Journal of Food Technology instname:Instituto de Tecnologia de Alimentos (ITAL) instacron:ITAL |
instname_str |
Instituto de Tecnologia de Alimentos (ITAL) |
instacron_str |
ITAL |
institution |
ITAL |
reponame_str |
Brazilian Journal of Food Technology |
collection |
Brazilian Journal of Food Technology |
repository.name.fl_str_mv |
Brazilian Journal of Food Technology - Instituto de Tecnologia de Alimentos (ITAL) |
repository.mail.fl_str_mv |
bjftsec@ital.sp.gov.br||bjftsec@ital.sp.gov.br |
_version_ |
1752128701678485504 |