Aflatoxigenic fungi and aflatoxin in cocoa
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório do Instituto de Tecnologia de Alimentos |
Texto Completo: | http://repositorio.ital.sp.gov.br/jspui/handle/123456789/602 |
Resumo: | This paper reports the occurrence of aflatoxigenic fungi and the presence of aflatoxins in 226 cocoa samples collected on Brazilian farms. The samples were taken at various stages of fermentation, drying and storage. A total of 819 potentially aflatoxigenic fungi were isolated using Dichloran 18% Glycerol agar after surface disinfection, and identified by standard techniques. The ability of the fungi to produce aflatoxins was determined using the agar plug technique and TLC. The presence of aflatoxins in cocoa samples was determined by HPLC using post-column derivatization with bromide after immunoaffinity column clean up. The aflatoxigenic fungi isolated were Aspergillus flavus, A. parasiticus and A. nomius. A considerable increase in numbers of these species was observed during drying and storage. In spite of the high prevalence of aflatoxigenic fungi, only low levels of aflatoxin were found in the cocoa samples, suggesting the existence of limiting factors to the accumulation of aflatoxins in the beans. |
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Aflatoxigenic fungi and aflatoxin in cocoaAflatoxinHPLCAflatoxigenic fungiTheobroma cacaoAspergillus flavusAspergillus parasiticusChocolateThis paper reports the occurrence of aflatoxigenic fungi and the presence of aflatoxins in 226 cocoa samples collected on Brazilian farms. The samples were taken at various stages of fermentation, drying and storage. A total of 819 potentially aflatoxigenic fungi were isolated using Dichloran 18% Glycerol agar after surface disinfection, and identified by standard techniques. The ability of the fungi to produce aflatoxins was determined using the agar plug technique and TLC. The presence of aflatoxins in cocoa samples was determined by HPLC using post-column derivatization with bromide after immunoaffinity column clean up. The aflatoxigenic fungi isolated were Aspergillus flavus, A. parasiticus and A. nomius. A considerable increase in numbers of these species was observed during drying and storage. In spite of the high prevalence of aflatoxigenic fungi, only low levels of aflatoxin were found in the cocoa samples, suggesting the existence of limiting factors to the accumulation of aflatoxins in the beans.ElsevierCopetti, Marina V.; et al.2023-01-30T19:54:14Z2023-01-30T19:54:14Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfInternational Journal of Food Microbiology, Oxford, v. 148, n. 2, p. 141-144, 2011.http://repositorio.ital.sp.gov.br/jspui/handle/123456789/602reponame:Repositório do Instituto de Tecnologia de Alimentosinstname:Instituto de Tecnologia de Alimentos (ITAL)instacron:ITALenginfo:eu-repo/semantics/openAccess2023-01-30T19:54:14Zoai:http://repositorio.ital.sp.gov.br:123456789/602Repositório InstitucionalPUBhttp://repositorio.ital.sp.gov.br/oai/requestbjftsec@ital.sp.gov.br || bjftsec@ital.sp.gov.bropendoar:2023-01-30T19:54:14Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL)false |
dc.title.none.fl_str_mv |
Aflatoxigenic fungi and aflatoxin in cocoa |
title |
Aflatoxigenic fungi and aflatoxin in cocoa |
spellingShingle |
Aflatoxigenic fungi and aflatoxin in cocoa Copetti, Marina V.; et al. Aflatoxin HPLC Aflatoxigenic fungi Theobroma cacao Aspergillus flavus Aspergillus parasiticus Chocolate |
title_short |
Aflatoxigenic fungi and aflatoxin in cocoa |
title_full |
Aflatoxigenic fungi and aflatoxin in cocoa |
title_fullStr |
Aflatoxigenic fungi and aflatoxin in cocoa |
title_full_unstemmed |
Aflatoxigenic fungi and aflatoxin in cocoa |
title_sort |
Aflatoxigenic fungi and aflatoxin in cocoa |
author |
Copetti, Marina V.; et al. |
author_facet |
Copetti, Marina V.; et al. |
author_role |
author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Copetti, Marina V.; et al. |
dc.subject.none.fl_str_mv |
|
dc.subject.por.fl_str_mv |
Aflatoxin HPLC Aflatoxigenic fungi Theobroma cacao Aspergillus flavus Aspergillus parasiticus Chocolate |
topic |
Aflatoxin HPLC Aflatoxigenic fungi Theobroma cacao Aspergillus flavus Aspergillus parasiticus Chocolate |
description |
This paper reports the occurrence of aflatoxigenic fungi and the presence of aflatoxins in 226 cocoa samples collected on Brazilian farms. The samples were taken at various stages of fermentation, drying and storage. A total of 819 potentially aflatoxigenic fungi were isolated using Dichloran 18% Glycerol agar after surface disinfection, and identified by standard techniques. The ability of the fungi to produce aflatoxins was determined using the agar plug technique and TLC. The presence of aflatoxins in cocoa samples was determined by HPLC using post-column derivatization with bromide after immunoaffinity column clean up. The aflatoxigenic fungi isolated were Aspergillus flavus, A. parasiticus and A. nomius. A considerable increase in numbers of these species was observed during drying and storage. In spite of the high prevalence of aflatoxigenic fungi, only low levels of aflatoxin were found in the cocoa samples, suggesting the existence of limiting factors to the accumulation of aflatoxins in the beans. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2023-01-30T19:54:14Z 2023-01-30T19:54:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
|
dc.identifier.uri.fl_str_mv |
International Journal of Food Microbiology, Oxford, v. 148, n. 2, p. 141-144, 2011. http://repositorio.ital.sp.gov.br/jspui/handle/123456789/602 |
identifier_str_mv |
International Journal of Food Microbiology, Oxford, v. 148, n. 2, p. 141-144, 2011. |
url |
http://repositorio.ital.sp.gov.br/jspui/handle/123456789/602 |
dc.language.none.fl_str_mv |
|
dc.language.iso.fl_str_mv |
eng |
language_invalid_str_mv |
|
language |
eng |
dc.rights.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
|
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório do Instituto de Tecnologia de Alimentos instname:Instituto de Tecnologia de Alimentos (ITAL) instacron:ITAL |
instname_str |
Instituto de Tecnologia de Alimentos (ITAL) |
instacron_str |
ITAL |
institution |
ITAL |
reponame_str |
Repositório do Instituto de Tecnologia de Alimentos |
collection |
Repositório do Instituto de Tecnologia de Alimentos |
repository.name.fl_str_mv |
Repositório do Instituto de Tecnologia de Alimentos - Instituto de Tecnologia de Alimentos (ITAL) |
repository.mail.fl_str_mv |
bjftsec@ital.sp.gov.br || bjftsec@ital.sp.gov.br |
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