Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis

Detalhes bibliográficos
Autor(a) principal: Araujo,Marlon Heggdorne de
Data de Publicação: 2021
Outros Autores: Simão,Thatiana Lopes Biá Ventura, Konno,Tatiana Ungaretti Paleo, Guimarães,Denise Oliveira, Leal,Ivana Correa Ramos, Lasunskaia,Elena, Muzitano,Michelle Frazão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Rodriguésia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100235
Resumo: Abstract Tuberculosis (TB) still constitutes a threat to public health in various regions of the world. The existing treatment is long and has many side effects. The need to identify new anti-TB compounds and also adjuvants to control exacerbated inflammation in severe TB cases is relevant. Therefore, the objective of this study was to evaluate the anti-mycobacterial activity of extracts and fractions in vitro from plant species collected in the Restinga of Jurubatiba, in Rio de Janeiro state, Brazil. In addition, to verify their immunomodulatory action and cytotoxicity on macrophages. The dichloromethane fraction of Kielmeyera membranacea and Eremanthus crotonoides showed the lowest MIC50 against Mycobacterium bovis BCG (0.95 ± 1.08 and 2.17 ± 1.11 μg/mL, respectively) and M. tuberculosis H37Rv (4.38 ± 1.19 and 15.28 ± 1.21 μg/mL, respectively). They were also able to inhibit the NO and TNF-α production in LPS-stimulated macrophages, without being toxic to cells. Using gas chromatography analysis coupled with mass spectrometer it was possible to suggest the presence of fatty acids and terpenes in the most promising fractions. Those compounds have been described for their anti-mycobacterial activity. These results have enabled identifying Kielmeyera membranacea and Eremanthus crotonoides as the most promising studied species in searching for new anti-TB compounds with dual activity.
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spelling Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosisanti-mycobacterialimmunomodulatoryrestingaterpenestuberculosisAbstract Tuberculosis (TB) still constitutes a threat to public health in various regions of the world. The existing treatment is long and has many side effects. The need to identify new anti-TB compounds and also adjuvants to control exacerbated inflammation in severe TB cases is relevant. Therefore, the objective of this study was to evaluate the anti-mycobacterial activity of extracts and fractions in vitro from plant species collected in the Restinga of Jurubatiba, in Rio de Janeiro state, Brazil. In addition, to verify their immunomodulatory action and cytotoxicity on macrophages. The dichloromethane fraction of Kielmeyera membranacea and Eremanthus crotonoides showed the lowest MIC50 against Mycobacterium bovis BCG (0.95 ± 1.08 and 2.17 ± 1.11 μg/mL, respectively) and M. tuberculosis H37Rv (4.38 ± 1.19 and 15.28 ± 1.21 μg/mL, respectively). They were also able to inhibit the NO and TNF-α production in LPS-stimulated macrophages, without being toxic to cells. Using gas chromatography analysis coupled with mass spectrometer it was possible to suggest the presence of fatty acids and terpenes in the most promising fractions. Those compounds have been described for their anti-mycobacterial activity. These results have enabled identifying Kielmeyera membranacea and Eremanthus crotonoides as the most promising studied species in searching for new anti-TB compounds with dual activity.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100235Rodriguésia v.72 2021reponame:Rodriguésia (Online)instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)instacron:JBRJ10.1590/2175-7860202172040info:eu-repo/semantics/openAccessAraujo,Marlon Heggdorne deSimão,Thatiana Lopes Biá VenturaKonno,Tatiana Ungaretti PaleoGuimarães,Denise OliveiraLeal,Ivana Correa RamosLasunskaia,ElenaMuzitano,Michelle Frazãoeng2021-12-08T00:00:00Zoai:scielo:S2175-78602021000100235Revistahttp://rodriguesia.jbrj.gov.br/https://old.scielo.br/oai/scielo-oai.phprodriguesia@jbrj.gov.br2175-78600370-6583opendoar:2021-12-08T00:00Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)false
dc.title.none.fl_str_mv Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
title Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
spellingShingle Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
Araujo,Marlon Heggdorne de
anti-mycobacterial
immunomodulatory
restinga
terpenes
tuberculosis
title_short Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
title_full Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
title_fullStr Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
title_full_unstemmed Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
title_sort Anti-mycobacterial and anti-inflammatory activity of restinga plants: a dual approach in searching for new drugs to treat severe tuberculosis
author Araujo,Marlon Heggdorne de
author_facet Araujo,Marlon Heggdorne de
Simão,Thatiana Lopes Biá Ventura
Konno,Tatiana Ungaretti Paleo
Guimarães,Denise Oliveira
Leal,Ivana Correa Ramos
Lasunskaia,Elena
Muzitano,Michelle Frazão
author_role author
author2 Simão,Thatiana Lopes Biá Ventura
Konno,Tatiana Ungaretti Paleo
Guimarães,Denise Oliveira
Leal,Ivana Correa Ramos
Lasunskaia,Elena
Muzitano,Michelle Frazão
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo,Marlon Heggdorne de
Simão,Thatiana Lopes Biá Ventura
Konno,Tatiana Ungaretti Paleo
Guimarães,Denise Oliveira
Leal,Ivana Correa Ramos
Lasunskaia,Elena
Muzitano,Michelle Frazão
dc.subject.por.fl_str_mv anti-mycobacterial
immunomodulatory
restinga
terpenes
tuberculosis
topic anti-mycobacterial
immunomodulatory
restinga
terpenes
tuberculosis
description Abstract Tuberculosis (TB) still constitutes a threat to public health in various regions of the world. The existing treatment is long and has many side effects. The need to identify new anti-TB compounds and also adjuvants to control exacerbated inflammation in severe TB cases is relevant. Therefore, the objective of this study was to evaluate the anti-mycobacterial activity of extracts and fractions in vitro from plant species collected in the Restinga of Jurubatiba, in Rio de Janeiro state, Brazil. In addition, to verify their immunomodulatory action and cytotoxicity on macrophages. The dichloromethane fraction of Kielmeyera membranacea and Eremanthus crotonoides showed the lowest MIC50 against Mycobacterium bovis BCG (0.95 ± 1.08 and 2.17 ± 1.11 μg/mL, respectively) and M. tuberculosis H37Rv (4.38 ± 1.19 and 15.28 ± 1.21 μg/mL, respectively). They were also able to inhibit the NO and TNF-α production in LPS-stimulated macrophages, without being toxic to cells. Using gas chromatography analysis coupled with mass spectrometer it was possible to suggest the presence of fatty acids and terpenes in the most promising fractions. Those compounds have been described for their anti-mycobacterial activity. These results have enabled identifying Kielmeyera membranacea and Eremanthus crotonoides as the most promising studied species in searching for new anti-TB compounds with dual activity.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100235
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100235
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2175-7860202172040
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
publisher.none.fl_str_mv Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
dc.source.none.fl_str_mv Rodriguésia v.72 2021
reponame:Rodriguésia (Online)
instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)
instacron:JBRJ
instname_str Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)
instacron_str JBRJ
institution JBRJ
reponame_str Rodriguésia (Online)
collection Rodriguésia (Online)
repository.name.fl_str_mv Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)
repository.mail.fl_str_mv rodriguesia@jbrj.gov.br
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