Hippocampal proteomic profile in temporal lobe epilepsy
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of epilepsy and clinical neurophysiology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-26492012000200007 |
Resumo: | In this study we used proteomics approaches to obtain the protein profile of human epileptic hippocampi (N=6) and control hippocampi obtained from autopsy (N=6). We used two-dimensional gel electrophoresis (2-D) coupled to HPLC and Mass spectroscopy (MALDI-TOF) to identify proteins differentially expressed. Nine proteins were differentially expressed comparing the hippocampus of patients with the hippocampus of control. Proteins that were increased in the hippocampus of patients with TLE were: isoform 1 of serum albumin, HSP 70, dihydropyrimidinase-related protein 2, isoform 1 of myelin basic protein, proton ATPase catalytic subunit A, and dihydrolipoyllysine-residue acethyltransferase component of pyruvate dehydrogenase complex. The expression of isoform 3 of spectrin alpha chain (fodrin) was down-regulated while the proteins glutathione S-transferase P and the DJ-1 (PARK7) were detected only in the hippocampus of patients with TLE. Taken together, our results provide evidence supporting a direct link between metabolic disturb and oxidative damage related to pathophysiology of TLE. Besides, indicates biomarkers for further investigations as therapies targeted to epilepsy. |
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Journal of epilepsy and clinical neurophysiology (Online) |
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Hippocampal proteomic profile in temporal lobe epilepsytemporal lobe epilepsyhippocampusproteomicsbiomarkersIn this study we used proteomics approaches to obtain the protein profile of human epileptic hippocampi (N=6) and control hippocampi obtained from autopsy (N=6). We used two-dimensional gel electrophoresis (2-D) coupled to HPLC and Mass spectroscopy (MALDI-TOF) to identify proteins differentially expressed. Nine proteins were differentially expressed comparing the hippocampus of patients with the hippocampus of control. Proteins that were increased in the hippocampus of patients with TLE were: isoform 1 of serum albumin, HSP 70, dihydropyrimidinase-related protein 2, isoform 1 of myelin basic protein, proton ATPase catalytic subunit A, and dihydrolipoyllysine-residue acethyltransferase component of pyruvate dehydrogenase complex. The expression of isoform 3 of spectrin alpha chain (fodrin) was down-regulated while the proteins glutathione S-transferase P and the DJ-1 (PARK7) were detected only in the hippocampus of patients with TLE. Taken together, our results provide evidence supporting a direct link between metabolic disturb and oxidative damage related to pathophysiology of TLE. Besides, indicates biomarkers for further investigations as therapies targeted to epilepsy.Liga Brasileira de Epilepsia (LBE)2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-26492012000200007Journal of Epilepsy and Clinical Neurophysiology v.18 n.2 2012reponame:Journal of epilepsy and clinical neurophysiology (Online)instname:Liga Brasileira de Epilepsia (LBE)instacron:LBE10.1590/S1676-26492012000200007info:eu-repo/semantics/openAccessPersike,DSLima,MLAmorim,RPCavalheiro,EAYacubian,EMTCenteno,RSCarrete Jr,HSchenkman,SCanzian,MFernandes,MJSeng2012-12-07T00:00:00Zoai:scielo:S1676-26492012000200007Revistahttp://epilepsia.org.br/publicacoes/ONGhttps://old.scielo.br/oai/scielo-oai.php||jecnpoa@terra.com.br1980-53651676-2649opendoar:2012-12-07T00:00Journal of epilepsy and clinical neurophysiology (Online) - Liga Brasileira de Epilepsia (LBE)false |
dc.title.none.fl_str_mv |
Hippocampal proteomic profile in temporal lobe epilepsy |
title |
Hippocampal proteomic profile in temporal lobe epilepsy |
spellingShingle |
Hippocampal proteomic profile in temporal lobe epilepsy Persike,DS temporal lobe epilepsy hippocampus proteomics biomarkers |
title_short |
Hippocampal proteomic profile in temporal lobe epilepsy |
title_full |
Hippocampal proteomic profile in temporal lobe epilepsy |
title_fullStr |
Hippocampal proteomic profile in temporal lobe epilepsy |
title_full_unstemmed |
Hippocampal proteomic profile in temporal lobe epilepsy |
title_sort |
Hippocampal proteomic profile in temporal lobe epilepsy |
author |
Persike,DS |
author_facet |
Persike,DS Lima,ML Amorim,RP Cavalheiro,EA Yacubian,EMT Centeno,RS Carrete Jr,H Schenkman,S Canzian,M Fernandes,MJS |
author_role |
author |
author2 |
Lima,ML Amorim,RP Cavalheiro,EA Yacubian,EMT Centeno,RS Carrete Jr,H Schenkman,S Canzian,M Fernandes,MJS |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Persike,DS Lima,ML Amorim,RP Cavalheiro,EA Yacubian,EMT Centeno,RS Carrete Jr,H Schenkman,S Canzian,M Fernandes,MJS |
dc.subject.por.fl_str_mv |
temporal lobe epilepsy hippocampus proteomics biomarkers |
topic |
temporal lobe epilepsy hippocampus proteomics biomarkers |
description |
In this study we used proteomics approaches to obtain the protein profile of human epileptic hippocampi (N=6) and control hippocampi obtained from autopsy (N=6). We used two-dimensional gel electrophoresis (2-D) coupled to HPLC and Mass spectroscopy (MALDI-TOF) to identify proteins differentially expressed. Nine proteins were differentially expressed comparing the hippocampus of patients with the hippocampus of control. Proteins that were increased in the hippocampus of patients with TLE were: isoform 1 of serum albumin, HSP 70, dihydropyrimidinase-related protein 2, isoform 1 of myelin basic protein, proton ATPase catalytic subunit A, and dihydrolipoyllysine-residue acethyltransferase component of pyruvate dehydrogenase complex. The expression of isoform 3 of spectrin alpha chain (fodrin) was down-regulated while the proteins glutathione S-transferase P and the DJ-1 (PARK7) were detected only in the hippocampus of patients with TLE. Taken together, our results provide evidence supporting a direct link between metabolic disturb and oxidative damage related to pathophysiology of TLE. Besides, indicates biomarkers for further investigations as therapies targeted to epilepsy. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-26492012000200007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-26492012000200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1676-26492012000200007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Liga Brasileira de Epilepsia (LBE) |
publisher.none.fl_str_mv |
Liga Brasileira de Epilepsia (LBE) |
dc.source.none.fl_str_mv |
Journal of Epilepsy and Clinical Neurophysiology v.18 n.2 2012 reponame:Journal of epilepsy and clinical neurophysiology (Online) instname:Liga Brasileira de Epilepsia (LBE) instacron:LBE |
instname_str |
Liga Brasileira de Epilepsia (LBE) |
instacron_str |
LBE |
institution |
LBE |
reponame_str |
Journal of epilepsy and clinical neurophysiology (Online) |
collection |
Journal of epilepsy and clinical neurophysiology (Online) |
repository.name.fl_str_mv |
Journal of epilepsy and clinical neurophysiology (Online) - Liga Brasileira de Epilepsia (LBE) |
repository.mail.fl_str_mv |
||jecnpoa@terra.com.br |
_version_ |
1754734659783098368 |