Sistema de comparação de genomas de procariotos- SCGP

• Autor(a) principal: Souza, Celso Rangel
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do LNCC
• Texto Completo: https://tede.lncc.br/handle/tede/298
• topic: Bioinformática; Genomas de procarioto; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

The availability of complete and partial genomes of prokaryotes has increased exponentially in public biological databases due to advances in genome sequencing techniques in recent years. Two years ago, after the installation of an infrastructure for DNA sequencing, the Bioinformatics Laboratory of the National Laboratory for Scientific Computation entered this era of large-scale sequencing. The in-silico genome analysis using these sequences was produced with very different purposes, such as comparative analysis of genomes, studies of metabolic pathways, phylogenetic analysis, among others. The larger supply of phylogenetically close genomes may facilitate biological inferences, but requires the development of tools to facilitate and accelerate the annotation and comparison of genes. The System for the Comparison of Prokaryotic Genomes - SCGP groups all possible orthologous genes given a set of genomes, stores them in a database as ix well as allows queries of genomes selected (by some annotation data such as product, gene name, synonym and amino acid sequence). From the clusters generated the system can infer information about common genes between the genomes studied, gene order and functional classification. The system also allows easy updating and adding new genomes from a previously comparison. For validation of the system, 19 genomes from the order Mycoplasmatales were tested. It was possible to search information about similarity and sequence alignment. On the other hand, the analysis method from the clusters allowed access to the list of homologous sequences groups for identifying the possible evolutionary relationships, structure as well as function between the genomes studied.