Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux

Detalhes bibliográficos
Autor(a) principal: Oliveira, Ellen A. de S.
Data de Publicação: 2019
Outros Autores: Duarte, Heitor M., Oliveira, Juliana A. de S., Tâmega, Frederico T. de S., Coutinho, Ricardo, Soares, Angelica R., Biofouling, Benthic Ecology and Marine Biotechnology Meeting, 013., 2019, Arraial do Cabo (RJ)
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Produção Científica da Marinha do Brasil (RI-MB)
Texto Completo: http://www.repositorio.mar.mil.br/handle/ripcmb/844546
Resumo: Macroalgae are an important source of bioactive natural products with several biological activities including antibacterial, antiviral, antifungal and antifouling. The production of these compounds can be influenced by abiotic factors (temperature, light and nutrients) as well as biotic factors (competition and predation). The geniculate coralline red algae (Corallinales, Rhodophyta) have a worldwide distribution and great importance for reef ecosystems. Some compounds with biological activity have been discovered, but there are few studies about this group, mainly about the brazilian species. Thus, the aim of this study was to compare the lipophilic chemical profile of two Jania crassa populations and to evaluate the antibacterial potential against five fouling marine bacteria strains (Vibrio aestuarianus, Pseudoalteromonas elyakovii, Polaribacter irgensii, Pseudomonas fluorescens and Shewanella putrefaciens). The samples were collected on the rocky shores of Prainha and Anjos beaches (Arraial do Cabo - RJ), lyophilized and extracted with ethyl acetate and methanol (1:1) to yield the crude extract. The lipophilic extracts were analyzed by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). For the bioassays with marine bacteria, extracts in the natural concentration were solubilized in dichloromethane (DCM) and incorporated into filter paper disks (5mm). Discs with the antibiotic rifampicin were used as a positive control and disks with DCM disks were used as a negative control. The GC-MS data were analyzed by the statistical method of principal components analysis (PCA) and antibacterial activity was analyzed by one-way (ANOVA). The results of the TLC and GC-MS showed that the extract from Anjos beach presented greater chemical diversity. The PCA analysis identified two substances (l-(+)-ascorbic acid 2,6-dihexadecanoate and (3β) cholest- 5-en-3-ol) responsible by separation of macroalgae populations. Both the extracts inhibited the growth of all bacteria, but the sample from Anjos beach had the inhibition significantly higher (p<0,05) against two bacteria (P. fluorescens and S. putrefaciens). Our results showed for the first time the natural products composition and the antibacterial potential of geniculate calcareous algae brazilian populations.
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spelling Oliveira, Ellen A. de S.Duarte, Heitor M.Oliveira, Juliana A. de S.Tâmega, Frederico T. de S.Coutinho, RicardoSoares, Angelica R.Biofouling, Benthic Ecology and Marine Biotechnology Meeting, 013., 2019, Arraial do Cabo (RJ)2020-03-28T18:19:43Z2020-03-28T18:19:43Z2019http://www.repositorio.mar.mil.br/handle/ripcmb/844546Macroalgae are an important source of bioactive natural products with several biological activities including antibacterial, antiviral, antifungal and antifouling. The production of these compounds can be influenced by abiotic factors (temperature, light and nutrients) as well as biotic factors (competition and predation). The geniculate coralline red algae (Corallinales, Rhodophyta) have a worldwide distribution and great importance for reef ecosystems. Some compounds with biological activity have been discovered, but there are few studies about this group, mainly about the brazilian species. Thus, the aim of this study was to compare the lipophilic chemical profile of two Jania crassa populations and to evaluate the antibacterial potential against five fouling marine bacteria strains (Vibrio aestuarianus, Pseudoalteromonas elyakovii, Polaribacter irgensii, Pseudomonas fluorescens and Shewanella putrefaciens). The samples were collected on the rocky shores of Prainha and Anjos beaches (Arraial do Cabo - RJ), lyophilized and extracted with ethyl acetate and methanol (1:1) to yield the crude extract. The lipophilic extracts were analyzed by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). For the bioassays with marine bacteria, extracts in the natural concentration were solubilized in dichloromethane (DCM) and incorporated into filter paper disks (5mm). Discs with the antibiotic rifampicin were used as a positive control and disks with DCM disks were used as a negative control. The GC-MS data were analyzed by the statistical method of principal components analysis (PCA) and antibacterial activity was analyzed by one-way (ANOVA). The results of the TLC and GC-MS showed that the extract from Anjos beach presented greater chemical diversity. The PCA analysis identified two substances (l-(+)-ascorbic acid 2,6-dihexadecanoate and (3β) cholest- 5-en-3-ol) responsible by separation of macroalgae populations. Both the extracts inhibited the growth of all bacteria, but the sample from Anjos beach had the inhibition significantly higher (p<0,05) against two bacteria (P. fluorescens and S. putrefaciens). Our results showed for the first time the natural products composition and the antibacterial potential of geniculate calcareous algae brazilian populations.engInstituto de Estudos do Mar Almirante Paulo Moreira (IEAPM)Ciência, Tecnologia e InovaçãoAlgas MarinhasChemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamourouxinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Produção Científica da Marinha do Brasil (RI-MB)instname:Marinha do Brasil (MB)instacron:MBTEXTCoutinho, Ricardo_BIOINC2019_Chemical diversity and antibacterial activity....pdf.txtCoutinho, Ricardo_BIOINC2019_Chemical diversity and antibacterial activity....pdf.txtExtracted texttext/plain6https://www.repositorio.mar.mil.br/bitstream/ripcmb/844546/2/Coutinho%2c%20Ricardo_BIOINC2019_Chemical%20diversity%20and%20antibacterial%20activity....pdf.txt010845330ff5c8439e0a9b24de156554MD52THUMBNAILCoutinho, Ricardo_BIOINC2019_Chemical diversity and antibacterial activity....pdf.jpgCoutinho, Ricardo_BIOINC2019_Chemical diversity and antibacterial activity....pdf.jpgGenerated Thumbnailimage/jpeg1823https://www.repositorio.mar.mil.br/bitstream/ripcmb/844546/3/Coutinho%2c%20Ricardo_BIOINC2019_Chemical%20diversity%20and%20antibacterial%20activity....pdf.jpg212df7fa15b1457aeb370134c055f519MD53ORIGINALCoutinho, Ricardo_BIOINC2019_Chemical diversity and antibacterial activity....pdfCoutinho, Ricardo_BIOINC2019_Chemical diversity and antibacterial activity....pdfapplication/pdf677544https://www.repositorio.mar.mil.br/bitstream/ripcmb/844546/1/Coutinho%2c%20Ricardo_BIOINC2019_Chemical%20diversity%20and%20antibacterial%20activity....pdfb39b798a2913d2c138733fc0e7d58ee5MD51ripcmb/8445462022-09-23 16:48:17.962oai:www.repositorio.mar.mil.br:ripcmb/844546Repositório InstitucionalPUBhttps://www.repositorio.mar.mil.br/oai/requestdphdm.repositorio@marinha.mil.bropendoar:2022-09-23T19:48:17Repositório Institucional da Produção Científica da Marinha do Brasil (RI-MB) - Marinha do Brasil (MB)false
dc.title.pt_BR.fl_str_mv Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
title Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
spellingShingle Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
Oliveira, Ellen A. de S.
Algas Marinhas
Ciência, Tecnologia e Inovação
title_short Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
title_full Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
title_fullStr Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
title_full_unstemmed Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
title_sort Chemical diversity and antibacterial activity of the geniculate coralline algae Jania crassa J.V. Lamouroux
author Oliveira, Ellen A. de S.
author_facet Oliveira, Ellen A. de S.
Duarte, Heitor M.
Oliveira, Juliana A. de S.
Tâmega, Frederico T. de S.
Coutinho, Ricardo
Soares, Angelica R.
Biofouling, Benthic Ecology and Marine Biotechnology Meeting, 013., 2019, Arraial do Cabo (RJ)
author_role author
author2 Duarte, Heitor M.
Oliveira, Juliana A. de S.
Tâmega, Frederico T. de S.
Coutinho, Ricardo
Soares, Angelica R.
Biofouling, Benthic Ecology and Marine Biotechnology Meeting, 013., 2019, Arraial do Cabo (RJ)
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Ellen A. de S.
Duarte, Heitor M.
Oliveira, Juliana A. de S.
Tâmega, Frederico T. de S.
Coutinho, Ricardo
Soares, Angelica R.
Biofouling, Benthic Ecology and Marine Biotechnology Meeting, 013., 2019, Arraial do Cabo (RJ)
dc.subject.por.fl_str_mv Algas Marinhas
topic Algas Marinhas
Ciência, Tecnologia e Inovação
dc.subject.dgpm.none.fl_str_mv Ciência, Tecnologia e Inovação
description Macroalgae are an important source of bioactive natural products with several biological activities including antibacterial, antiviral, antifungal and antifouling. The production of these compounds can be influenced by abiotic factors (temperature, light and nutrients) as well as biotic factors (competition and predation). The geniculate coralline red algae (Corallinales, Rhodophyta) have a worldwide distribution and great importance for reef ecosystems. Some compounds with biological activity have been discovered, but there are few studies about this group, mainly about the brazilian species. Thus, the aim of this study was to compare the lipophilic chemical profile of two Jania crassa populations and to evaluate the antibacterial potential against five fouling marine bacteria strains (Vibrio aestuarianus, Pseudoalteromonas elyakovii, Polaribacter irgensii, Pseudomonas fluorescens and Shewanella putrefaciens). The samples were collected on the rocky shores of Prainha and Anjos beaches (Arraial do Cabo - RJ), lyophilized and extracted with ethyl acetate and methanol (1:1) to yield the crude extract. The lipophilic extracts were analyzed by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). For the bioassays with marine bacteria, extracts in the natural concentration were solubilized in dichloromethane (DCM) and incorporated into filter paper disks (5mm). Discs with the antibiotic rifampicin were used as a positive control and disks with DCM disks were used as a negative control. The GC-MS data were analyzed by the statistical method of principal components analysis (PCA) and antibacterial activity was analyzed by one-way (ANOVA). The results of the TLC and GC-MS showed that the extract from Anjos beach presented greater chemical diversity. The PCA analysis identified two substances (l-(+)-ascorbic acid 2,6-dihexadecanoate and (3β) cholest- 5-en-3-ol) responsible by separation of macroalgae populations. Both the extracts inhibited the growth of all bacteria, but the sample from Anjos beach had the inhibition significantly higher (p<0,05) against two bacteria (P. fluorescens and S. putrefaciens). Our results showed for the first time the natural products composition and the antibacterial potential of geniculate calcareous algae brazilian populations.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-03-28T18:19:43Z
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