Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1857 |
Resumo: | The actual scientific approach to bioengineering organs is based on the use of natural extracellular matrix of decellularized lung as initial departure for subsequent reconstruction of the organ for recellularization in a bioreactor. The decellularization technique can be performed by two infusion ways, one through the trachea and other by pulmonary artery. The aim of this studies was to investigate in an experimental animal model, by the occlusion at the end of inspiration (OEI) and movement equation (ME), the behavior of the elastic, viscous and viscoelastic mechanical properties, of mice lungs after the process of decellularization through the trachea and the pulmonary artery. Were used 30 male mice C57BL/6, weighing 17-18 g (7-8 weeks of age), anesthetized and euthanized by exsanguination through the abdominal aorta. The decellularization protocol comprised the following steps: collecting, cleaning, freezing and thawing, rinsing with sodium dodecyl sulfate and phosphate buffered saline. For the studies I and II, the lungs were divided into the control group (GC = 10), the decellularization group by the trachea (TDG = 10) and the pulmonary artery decellularization group (PDG = 10), 5 lungs in each group for the study I that had the analysis through the technique of OEI and 5 lungs in each group for the study II with analysis through the ME. In the study I, the values of static elastance (Eest = CG: 226.9 ?? 4.1, PDG: 162.6 ?? 3.9, TDG: 154.8 ?? 1.7) and dynamics (Edyn = CG: 240 , 9 ?? 6.7, PDG: 176 ?? 5.4, TDG: 177.6 ?? 1.6) were significantly lower in TDG and PDG when compared to CG. In study II, the value of lung resistance presented in TDG was significantly lower in relation to the other two groups (R = CG: 5.32 ?? 0.26; PDG: 5.94 ?? 0.24; TDG: 2.85 ?? 0.14) and GC elastance was significantly higher in comparison with TDG and PDG, and in PDG the difference was significantly lower in relation to TDG (E = CG: 279 ?? 13.81; PDG: 146 ?? 19.04, TDG: 209.6 ?? 12.06). We can observe in both the two-way decellularization are effective to provide a scaffold ideal for pulmonary later recellularization when evaluated by the OEI and ME. Furthermore, the decellularized lungs through the pulmonary artery may be used to create a pulmonary scaffold in less time, because the protocol can be performed in a day, which facilitates the process for obtaining functional lungs scaffolds. |
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Oliveira, Luis Vicente Franco deOliveira, Luis Vicente Franco deJorge, Luciana Maria Malos?? SampaioRomanholo, Beatriz Mangueira Saraivahttp://lattes.cnpq.br/1729366476886322Urbano, Jessica Julioti2018-07-17T21:13:23Z2016-11-30Urbano, Jessica Julioti. Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar. 2016. 86 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1857The actual scientific approach to bioengineering organs is based on the use of natural extracellular matrix of decellularized lung as initial departure for subsequent reconstruction of the organ for recellularization in a bioreactor. The decellularization technique can be performed by two infusion ways, one through the trachea and other by pulmonary artery. The aim of this studies was to investigate in an experimental animal model, by the occlusion at the end of inspiration (OEI) and movement equation (ME), the behavior of the elastic, viscous and viscoelastic mechanical properties, of mice lungs after the process of decellularization through the trachea and the pulmonary artery. Were used 30 male mice C57BL/6, weighing 17-18 g (7-8 weeks of age), anesthetized and euthanized by exsanguination through the abdominal aorta. The decellularization protocol comprised the following steps: collecting, cleaning, freezing and thawing, rinsing with sodium dodecyl sulfate and phosphate buffered saline. For the studies I and II, the lungs were divided into the control group (GC = 10), the decellularization group by the trachea (TDG = 10) and the pulmonary artery decellularization group (PDG = 10), 5 lungs in each group for the study I that had the analysis through the technique of OEI and 5 lungs in each group for the study II with analysis through the ME. In the study I, the values of static elastance (Eest = CG: 226.9 ?? 4.1, PDG: 162.6 ?? 3.9, TDG: 154.8 ?? 1.7) and dynamics (Edyn = CG: 240 , 9 ?? 6.7, PDG: 176 ?? 5.4, TDG: 177.6 ?? 1.6) were significantly lower in TDG and PDG when compared to CG. In study II, the value of lung resistance presented in TDG was significantly lower in relation to the other two groups (R = CG: 5.32 ?? 0.26; PDG: 5.94 ?? 0.24; TDG: 2.85 ?? 0.14) and GC elastance was significantly higher in comparison with TDG and PDG, and in PDG the difference was significantly lower in relation to TDG (E = CG: 279 ?? 13.81; PDG: 146 ?? 19.04, TDG: 209.6 ?? 12.06). We can observe in both the two-way decellularization are effective to provide a scaffold ideal for pulmonary later recellularization when evaluated by the OEI and ME. Furthermore, the decellularized lungs through the pulmonary artery may be used to create a pulmonary scaffold in less time, because the protocol can be performed in a day, which facilitates the process for obtaining functional lungs scaffolds.A abordagem cient??fica atual para a bioengenharia de ??rg??os ?? baseada na utiliza????o da matriz extracelular natural do pulm??o descelularizado como partida inicial para posterior reconstru????o do ??rg??o por recelulariza????o em um biorreator. A t??cnica de descelulariza????o pode ser realizada por duas vias de perfus??o, uma atrav??s da traqueia e a outra pela art??ria pulmonar. O objetivo deste estudo foi investigar em um modelo experimental animal, atrav??s da t??cnica de oclus??o ao final da inspira????o (OFI) e equa????o do movimento (EM), o comportamento das propriedades mec??nicas el??sticas, viscosas e viscoel??sticas de pulm??es descelularizados de camundongos pelos m??todos de perfus??o atrav??s da traqueia e da art??ria pulmonar. Foram utilizados 30 camundongos machos da ra??a C57BL/6, com peso de 17-18 g (7-8 semanas de idade), anestesiados e eutanasiados por exsanguina????o pela aorta abdominal. O protocolo de descelulariza????o seguiu as etapas de coleta, limpeza, congelamento e descongelamento, lavagem com dodecil-sulfato de s??dio e tamp??o fosfato-salino. Para a realiza????o dos estudos I e II, os pulm??es foram divididos em grupo controle (GC = 10), grupo descelulariza????o pela traqueia (TDG = 10) e grupo descelulariza????o pela art??ria pulmonar (PDG = 10), sendo 5 pulm??es em cada grupo para o estudo I que teve a an??lise atrav??s da t??cnica de OFI e 5 pulm??es em cada grupo para o estudo II com an??lise atrav??s da EM. No estudo I, os valores de elast??ncia est??tica (Eest = CG: 226,9 ?? 4,1; PDG: 162,6 ?? 3,9; TDG: 154,8 ?? 1,7) e din??mica (Edyn = CG: 240,9 ?? 6,7; PDG: 176 ?? 5,4; TDG: 177,6 ?? 1,6) foram significativamente menores em TDG e PDG quando comparados com o CG. J?? no estudo II, o valor da resist??ncia pulmonar apresentado no TDG foi significativamente menor em rela????o aos outros dois grupos (R = CG: 5,32 ?? 0,26; PDG: 5,94 ?? 0,24; TDG: 2,85 ?? 0,14) e o valor da elast??ncia de CG apresentou-se maior de forma significativa em compara????o com a TDG e PDG, e no PDG a diferen??a foi menor significativamente em rela????o ?? TDG (E = CG: 279 ?? 13,81; PDG: 146 ?? 19,04; TDG: 209,6 ?? 12,06). Podemos observar que, quando avaliadas pelas t??cnicas de OFI e EM, as duas vias de descelulariza????o foram eficazes na gera????o de um scaffold pulmonar ideal para posterior recelulariza????o. Al??m disso, a t??cnica de descelulariza????o atrav??s da art??ria pulmonar mostrou-se eficaz para a obten????o de um scaffold pulmonar em menor per??odo de tempo, uma vez que o protocolo pode ser realizado em um dia, o que facilita o processo de obten????o de pulm??es funcionais.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-07-17T21:13:23Z No. of bitstreams: 1 Jessica Julioti Urbano.pdf: 3065396 bytes, checksum: 1e38f15e601296f027d3fd4cd280d051 (MD5)Made available in DSpace on 2018-07-17T21:13:23Z (GMT). No. of bitstreams: 1 Jessica Julioti Urbano.pdf: 3065396 bytes, checksum: 1e38f15e601296f027d3fd4cd280d051 (MD5) Previous issue date: 2016-11-30application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??depulm??esdescelulariza????otraqueiaart??ria pulmonarmec??nica ventilat??rialungsdecellularizationtracheapulmonary arterymechanical ventilatoryCIENCIAS DA SAUDEResist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonarResistance and compliance of decellularized lungs of mice through the perfusion techniques of the trachea and pulmonary arteryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALJessica Julioti Urbano.pdfJessica Julioti Urbano.pdfapplication/pdf3065396http://localhost:8080/tede/bitstream/tede/1857/2/Jessica+Julioti+Urbano.pdf1e38f15e601296f027d3fd4cd280d051MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1857/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18572019-11-13 13:39:54.759oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2019-11-13T15:39:54Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
dc.title.alternative.eng.fl_str_mv |
Resistance and compliance of decellularized lungs of mice through the perfusion techniques of the trachea and pulmonary artery |
title |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
spellingShingle |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar Urbano, Jessica Julioti pulm??es descelulariza????o traqueia art??ria pulmonar mec??nica ventilat??ria lungs decellularization trachea pulmonary artery mechanical ventilatory CIENCIAS DA SAUDE |
title_short |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
title_full |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
title_fullStr |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
title_full_unstemmed |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
title_sort |
Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar |
author |
Urbano, Jessica Julioti |
author_facet |
Urbano, Jessica Julioti |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Oliveira, Luis Vicente Franco de |
dc.contributor.referee1.fl_str_mv |
Oliveira, Luis Vicente Franco de |
dc.contributor.referee2.fl_str_mv |
Jorge, Luciana Maria Malos?? Sampaio |
dc.contributor.referee3.fl_str_mv |
Romanholo, Beatriz Mangueira Saraiva |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1729366476886322 |
dc.contributor.author.fl_str_mv |
Urbano, Jessica Julioti |
contributor_str_mv |
Oliveira, Luis Vicente Franco de Oliveira, Luis Vicente Franco de Jorge, Luciana Maria Malos?? Sampaio Romanholo, Beatriz Mangueira Saraiva |
dc.subject.por.fl_str_mv |
pulm??es descelulariza????o traqueia art??ria pulmonar mec??nica ventilat??ria |
topic |
pulm??es descelulariza????o traqueia art??ria pulmonar mec??nica ventilat??ria lungs decellularization trachea pulmonary artery mechanical ventilatory CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
lungs decellularization trachea pulmonary artery mechanical ventilatory |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The actual scientific approach to bioengineering organs is based on the use of natural extracellular matrix of decellularized lung as initial departure for subsequent reconstruction of the organ for recellularization in a bioreactor. The decellularization technique can be performed by two infusion ways, one through the trachea and other by pulmonary artery. The aim of this studies was to investigate in an experimental animal model, by the occlusion at the end of inspiration (OEI) and movement equation (ME), the behavior of the elastic, viscous and viscoelastic mechanical properties, of mice lungs after the process of decellularization through the trachea and the pulmonary artery. Were used 30 male mice C57BL/6, weighing 17-18 g (7-8 weeks of age), anesthetized and euthanized by exsanguination through the abdominal aorta. The decellularization protocol comprised the following steps: collecting, cleaning, freezing and thawing, rinsing with sodium dodecyl sulfate and phosphate buffered saline. For the studies I and II, the lungs were divided into the control group (GC = 10), the decellularization group by the trachea (TDG = 10) and the pulmonary artery decellularization group (PDG = 10), 5 lungs in each group for the study I that had the analysis through the technique of OEI and 5 lungs in each group for the study II with analysis through the ME. In the study I, the values of static elastance (Eest = CG: 226.9 ?? 4.1, PDG: 162.6 ?? 3.9, TDG: 154.8 ?? 1.7) and dynamics (Edyn = CG: 240 , 9 ?? 6.7, PDG: 176 ?? 5.4, TDG: 177.6 ?? 1.6) were significantly lower in TDG and PDG when compared to CG. In study II, the value of lung resistance presented in TDG was significantly lower in relation to the other two groups (R = CG: 5.32 ?? 0.26; PDG: 5.94 ?? 0.24; TDG: 2.85 ?? 0.14) and GC elastance was significantly higher in comparison with TDG and PDG, and in PDG the difference was significantly lower in relation to TDG (E = CG: 279 ?? 13.81; PDG: 146 ?? 19.04, TDG: 209.6 ?? 12.06). We can observe in both the two-way decellularization are effective to provide a scaffold ideal for pulmonary later recellularization when evaluated by the OEI and ME. Furthermore, the decellularized lungs through the pulmonary artery may be used to create a pulmonary scaffold in less time, because the protocol can be performed in a day, which facilitates the process for obtaining functional lungs scaffolds. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-11-30 |
dc.date.accessioned.fl_str_mv |
2018-07-17T21:13:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Urbano, Jessica Julioti. Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar. 2016. 86 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1857 |
identifier_str_mv |
Urbano, Jessica Julioti. Resist??ncia e complac??ncia de pulm??es descelularizados de camundongos atrav??s das t??cnicas de perfus??o pela traqueia e art??ria pulmonar. 2016. 86 f. Disserta????o( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/1857 |
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openAccess |
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dc.publisher.program.fl_str_mv |
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UNINOVE |
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