Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1776 |
Resumo: | Skeletal muscle is a tissue of great adaptive capacity and is able to change its characteristics to meet various functional demands. This adaptation can be caused by mechanical overloading which results in an increase in the size of the area of muscle fiber and increase muscle mass. This process is known as muscle hypertrophy and during numerous modifications hypertrophy occur in both the muscle tissue and tendons. Studies with low-level laser (LLL) in skeletal muscle have demonstrated that its effects are positive with respect to the reduction process of inflammation, myonecrosis and reduced influence on the collagen fibers during the remodeling process.The present study aimed to analyze the effects of LLL (780nm) during the compensatory hypertrophy process on the muscular aspects and tendon of the plantaris muscle of mice (in vivo) and also evaluate its effects on human endothelial cells (HUVECs) in vitro. They were used for the in vivo experiment 22 Wistar rats were divided into control group, non-irradiated group and irradiated group hypertrophy hypertrophy. The parameters used for the irradiation were ?? = 780nm, beam area 0.04 cm2, 40mW output power, energy density of 10 J / cm2. periods of 7 and 14 days were analyzed. To cause hypertrophy model was used for ablation of synergists being taken for this study, the gastrocnemius medial and lateral muscles and the soleus muscle, leaving the plantaris muscle suffered overload. the cross-sectional area were analyzed, collagen area, the number of myonuclei and mature blood vessels in muscle tissue and the organization and arrangement of collagen fibers in the tendon. For the experiment in vitro endothelial cells were used (HUVECs) were divided into control group and the other groups irradiated also with ?? = 780nm, 0.04 cm2 beam area, 40mW output power and energy density of 1 J / cm2, 5J / cm2, 10 J / cm2 to 20J / cm2. The different application time was calculated for all groups received the same dose of energy. They assessed cell viability, total protein and vessel formation in Matrigel. The in vivo results showed that there was increased cross-sectional area of the irradiated group after 14 days (26.3%) when compared to non-irradiated group. There was also increased number of myonuclei in the irradiated group after 14 days. The total area of muscle collagen increased (4.2%) in the irradiated group in 7 days and decreased (6.4%) in the irradiated group after 14 days when compared to non-irradiated group in both periods. Analysis by birefringence in the tendons showed better organization of the fibers 7 and 14 days when compared to non-irradiated group. In vitro results have shown decrease in cell viability in all irradiated all groups compared to the control and counting mature vessels was increased number of vessels in the irradiated group even after 14 days. In conclusion laser irradiation increased muscle mass, the number of myonuclei and the number of mature vessels and decreased collagen total area of muscle tissue after 14 days; improved tendon collagen organization for 7 and 14 days and interfere with the viability and total protein concentration in a dose-dependent endothelial cells HUVECs. |
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Ferrari, Raquel Agnelli MesquitaFernandes, Kristianne Porta SantosFerrari, Raquel Agnelli MesquitaFernandes, Kristianne Porta SantosFernandes, Maria Helena RaposoCorrea, LucianaMarcos, Rodrigo Labathttp://lattes.cnpq.br/6431622897125543Terena, Stella Maris Lins2018-06-14T20:50:43Z2016-06-28Terena, Stella Maris Lins. Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro. 2016. 69 f. Tese( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1776Skeletal muscle is a tissue of great adaptive capacity and is able to change its characteristics to meet various functional demands. This adaptation can be caused by mechanical overloading which results in an increase in the size of the area of muscle fiber and increase muscle mass. This process is known as muscle hypertrophy and during numerous modifications hypertrophy occur in both the muscle tissue and tendons. Studies with low-level laser (LLL) in skeletal muscle have demonstrated that its effects are positive with respect to the reduction process of inflammation, myonecrosis and reduced influence on the collagen fibers during the remodeling process.The present study aimed to analyze the effects of LLL (780nm) during the compensatory hypertrophy process on the muscular aspects and tendon of the plantaris muscle of mice (in vivo) and also evaluate its effects on human endothelial cells (HUVECs) in vitro. They were used for the in vivo experiment 22 Wistar rats were divided into control group, non-irradiated group and irradiated group hypertrophy hypertrophy. The parameters used for the irradiation were ?? = 780nm, beam area 0.04 cm2, 40mW output power, energy density of 10 J / cm2. periods of 7 and 14 days were analyzed. To cause hypertrophy model was used for ablation of synergists being taken for this study, the gastrocnemius medial and lateral muscles and the soleus muscle, leaving the plantaris muscle suffered overload. the cross-sectional area were analyzed, collagen area, the number of myonuclei and mature blood vessels in muscle tissue and the organization and arrangement of collagen fibers in the tendon. For the experiment in vitro endothelial cells were used (HUVECs) were divided into control group and the other groups irradiated also with ?? = 780nm, 0.04 cm2 beam area, 40mW output power and energy density of 1 J / cm2, 5J / cm2, 10 J / cm2 to 20J / cm2. The different application time was calculated for all groups received the same dose of energy. They assessed cell viability, total protein and vessel formation in Matrigel. The in vivo results showed that there was increased cross-sectional area of the irradiated group after 14 days (26.3%) when compared to non-irradiated group. There was also increased number of myonuclei in the irradiated group after 14 days. The total area of muscle collagen increased (4.2%) in the irradiated group in 7 days and decreased (6.4%) in the irradiated group after 14 days when compared to non-irradiated group in both periods. Analysis by birefringence in the tendons showed better organization of the fibers 7 and 14 days when compared to non-irradiated group. In vitro results have shown decrease in cell viability in all irradiated all groups compared to the control and counting mature vessels was increased number of vessels in the irradiated group even after 14 days. In conclusion laser irradiation increased muscle mass, the number of myonuclei and the number of mature vessels and decreased collagen total area of muscle tissue after 14 days; improved tendon collagen organization for 7 and 14 days and interfere with the viability and total protein concentration in a dose-dependent endothelial cells HUVECs.O m??sculo esquel??tico ?? um tecido de grande capacidade adaptativa e que ?? capaz de alterar suas caracter??sticas para atender ??s diversas demandas funcionais. Essa adapta????o pode ser causada por uma sobrecarga mec??nica que resulta num aumento do tamanho da ??rea da fibra muscular e aumento de massa muscular. Esse processo ?? conhecido como hipertrofia muscular e durante a hipertrofia in??meras modifica????es ocorrem tanto no tecido muscular quanto nos tend??es. Os trabalhos recentes com laser de baixa pot??ncia (LBP) no m??sculo esquel??tico tem demonstrado que seus efeitos s??o positivos no que diz respeito ao processo de redu????o da inflama????o, redu????o da mionecrose e influ??ncia sobre as fibras de col??geno durante o processo de remodelamento. Nesse contexto o presente estudo teve por objetivo analisar os efeitos do LBP (780nm) durante o processo de hipertrofia compensat??ria sobre os aspectos m??sculares e tend??neos do m??sculo plantar de ratos (in vivo) e tamb??m avaliar seus efeitos em c??lulas endoteliais humanas (Huvecs) in vitro. Foram utilizados para o experimento in vivo 22 ratos Wistar, divididos em 3 grupos: controle, n??o irradiado com hipertrofia e irradiado com hipertrofia. Os par??metros utilizados para a irradia????o foram ??= 780nm, ??rea do feixe 0,04 cm2, pot??ncia de sa??da 40mW, densidade de energia de 10J/cm2. Foram analisados os per??odos de 7 e 14 dias. Para causar a hipertrofia foi utilizado o modelo de abla????o dos sinergistas sendo retirados para este estudo os m??sculos gastrocn??mio lateral e medial e o m??sculo s??leo, restando o m??sculo plantar que sofreu a sobrecarga. Foram analisados a ??rea de sec????o transversa, a ??rea do col??geno, o n??mero de mion??cleos e vasos sangu??neos maduros no tecido muscular e organiza????o e disposi????o das fibras de col??geno no tend??o. Para o experimento in vitro foram utilizadas c??lulas endoteliais (Huvecs) divididas em grupos controle e os demais grupos irradiados tamb??m com ??= 780nm, ??rea do feixe 0,04 cm2, pot??ncia de sa??da 40mW e densidades de energia de 1J/cm2, 5J/cm2, 10J/cm2 e 20J/cm2. O tempo de aplica????o foi diferente, calculado para que todos os grupos recebessem a mesma dose de energia. Foram avaliados a viabilidade celular, prote??na total e a forma????o de vasos em matrigel. Os resultados in vivo obtidos demonstraram que houve aumento da ??rea de sec????o transversa do grupo irradiado ap??s 14 dias (26,3%) quando comparado ao grupo n??o irradiado. Houve tamb??m aumento do n??mero de mion??cleos no grupo irradiado ap??s 14 dias. A ??rea total do col??geno muscular aumentou (4,2%) no grupo irradiado em 7 dias e diminui (6,4%) no grupo irradiado ap??s 14 dias quando comparado ao grupo n??o irradiado nos dois per??odos. A an??lise por birrefring??ncia nos tend??es demonstrou maior organiza????o das fibras em 7 e em 14 dias quando comparadas ao grupo n??o irradiado. Os resultados in vitro demonstraram que as diferentes flu??ncias do laser interferiram de maneira diferente na viabilidade de c??lulas Huvecs em todos os per??odos avaliados. Com rela????o a contagem de vasos maduros houve aumento no grupo irradiado ap??s 14 dias. Em conclus??o a irradia????o laser aumentou a massa muscular, o n??mero de mion??cleos e o n??mero de vasos maduros e diminuiu a ??rea total de col??geno no tecido muscular ap??s 14 dias; melhorou a organiza????o do col??geno tend??neo em 7 e em 14 dias e interferiu na viabilidade e concentra????o de prote??na total de maneira dose dependente das c??lulas endoteliais Huvecs.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-14T20:50:43Z No. of bitstreams: 1 Stella Maris Lins Terena.pdf: 2089344 bytes, checksum: 21a72af34c64206cc3ab2d9b66964a22 (MD5)Made available in DSpace on 2018-06-14T20:50:43Z (GMT). No. of bitstreams: 1 Stella Maris Lins Terena.pdf: 2089344 bytes, checksum: 21a72af34c64206cc3ab2d9b66964a22 (MD5) Previous issue date: 2016-06-28application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??deUNINOVEBrasilSa??decol??genocomplexo m??sculo-tend??neohipertrofia compensat??rialaser de baixa pot??nciaHuvecscollagenmuscle-tendon complexcompensatory hypertrophylow-level laserHuvecsCIENCIAS DA SAUDEEfeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitroEffects of low-level laser (780nm) on the morphology of the tendon muscle complex in plantar muscle of rats during the process of compensatory hypertrophy: in vivo and in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALStella Maris Lins Terena.pdfStella Maris Lins Terena.pdfapplication/pdf2089344http://localhost:8080/tede/bitstream/tede/1776/2/Stella+Maris+Lins+Terena.pdf21a72af34c64206cc3ab2d9b66964a22MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1776/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/17762018-06-14 17:50:43.846oai:localhost:tede/1776Tk9UQTogQ09MT1FVRSBBUVVJIEEgU1VBIFBSPz9QUklBIExJQ0VOPz9BCkVzdGEgbGljZW4/P2EgZGUgZXhlbXBsbyA/PyBmb3JuZWNpZGEgYXBlbmFzIHBhcmEgZmlucyBpbmZvcm1hdGl2b3MuCgpMSUNFTj8/QSBERSBESVNUUklCVUk/Pz8/TyBOPz9PLUVYQ0xVU0lWQQoKQ29tIGEgYXByZXNlbnRhPz8/P28gZGVzdGEgbGljZW4/P2EsIHZvYz8/IChvIGF1dG9yIChlcykgb3UgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBkZSBhdXRvcikgY29uY2VkZSA/PyBVbml2ZXJzaWRhZGUgClhYWCAoU2lnbGEgZGEgVW5pdmVyc2lkYWRlKSBvIGRpcmVpdG8gbj8/by1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cj8/bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3MgPz91ZGlvIG91IHY/P2Rlby4KClZvYz8/IGNvbmNvcmRhIHF1ZSBhIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBwb2RlLCBzZW0gYWx0ZXJhciBvIGNvbnRlPz9kbywgdHJhbnNwb3IgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YT8/Pz9vIApwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmE/Pz8/by4KClZvYz8/IHRhbWI/P20gY29uY29yZGEgcXVlIGEgU2lnbGEgZGUgVW5pdmVyc2lkYWRlIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGM/P3BpYSBhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YT8/Pz9vIHBhcmEgZmlucyBkZSBzZWd1cmFuPz9hLCBiYWNrLXVwIGUgcHJlc2VydmE/Pz8/by4KClZvYz8/IGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byA/PyBvcmlnaW5hbCBlIHF1ZSB2b2M/PyB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcyAKbmVzdGEgbGljZW4/P2EuIFZvYz8/IHRhbWI/P20gZGVjbGFyYSBxdWUgbyBkZXA/P3NpdG8gZGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byBuPz9vLCBxdWUgc2VqYSBkZSBzZXUgCmNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgZGUgbmluZ3U/P20uCgpDYXNvIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGE/Pz8/byBjb250ZW5oYSBtYXRlcmlhbCBxdWUgdm9jPz8gbj8/byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2M/PyAKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzcz8/byBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyID8/IFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2VuPz9hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Q/PyBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZT8/ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YT8/Pz9vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEE/Pz8/TyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0M/P05JTyBPVSAKQVBPSU8gREUgVU1BIEFHPz9OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOPz9PIFNFSkEgQSBTSUdMQSBERSAKVU5JVkVSU0lEQURFLCBWT0M/PyBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVM/P08gQ09NTyAKVEFNQj8/TSBBUyBERU1BSVMgT0JSSUdBPz8/P0VTIEVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpBIFNpZ2xhIGRlIFVuaXZlcnNpZGFkZSBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lIChzKSBvdSBvKHMpIG5vbWUocykgZG8ocykgCmRldGVudG9yKGVzKSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgZGEgdGVzZSBvdSBkaXNzZXJ0YT8/Pz9vLCBlIG4/P28gZmFyPz8gcXVhbHF1ZXIgYWx0ZXJhPz8/P28sIGFsPz9tIGRhcXVlbGFzIApjb25jZWRpZGFzIHBvciBlc3RhIGxpY2VuPz9hLgo=Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-14T20:50:43Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
dc.title.alternative.eng.fl_str_mv |
Effects of low-level laser (780nm) on the morphology of the tendon muscle complex in plantar muscle of rats during the process of compensatory hypertrophy: in vivo and in vitro |
title |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
spellingShingle |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro Terena, Stella Maris Lins col??geno complexo m??sculo-tend??neo hipertrofia compensat??ria laser de baixa pot??ncia Huvecs collagen muscle-tendon complex compensatory hypertrophy low-level laser Huvecs CIENCIAS DA SAUDE |
title_short |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
title_full |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
title_fullStr |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
title_full_unstemmed |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
title_sort |
Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro |
author |
Terena, Stella Maris Lins |
author_facet |
Terena, Stella Maris Lins |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor-co1.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.referee1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee2.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.referee3.fl_str_mv |
Fernandes, Maria Helena Raposo |
dc.contributor.referee4.fl_str_mv |
Correa, Luciana |
dc.contributor.referee5.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6431622897125543 |
dc.contributor.author.fl_str_mv |
Terena, Stella Maris Lins |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Fernandes, Kristianne Porta Santos Ferrari, Raquel Agnelli Mesquita Fernandes, Kristianne Porta Santos Fernandes, Maria Helena Raposo Correa, Luciana Marcos, Rodrigo Labat |
dc.subject.por.fl_str_mv |
col??geno complexo m??sculo-tend??neo hipertrofia compensat??ria laser de baixa pot??ncia Huvecs |
topic |
col??geno complexo m??sculo-tend??neo hipertrofia compensat??ria laser de baixa pot??ncia Huvecs collagen muscle-tendon complex compensatory hypertrophy low-level laser Huvecs CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
collagen muscle-tendon complex compensatory hypertrophy low-level laser Huvecs |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Skeletal muscle is a tissue of great adaptive capacity and is able to change its characteristics to meet various functional demands. This adaptation can be caused by mechanical overloading which results in an increase in the size of the area of muscle fiber and increase muscle mass. This process is known as muscle hypertrophy and during numerous modifications hypertrophy occur in both the muscle tissue and tendons. Studies with low-level laser (LLL) in skeletal muscle have demonstrated that its effects are positive with respect to the reduction process of inflammation, myonecrosis and reduced influence on the collagen fibers during the remodeling process.The present study aimed to analyze the effects of LLL (780nm) during the compensatory hypertrophy process on the muscular aspects and tendon of the plantaris muscle of mice (in vivo) and also evaluate its effects on human endothelial cells (HUVECs) in vitro. They were used for the in vivo experiment 22 Wistar rats were divided into control group, non-irradiated group and irradiated group hypertrophy hypertrophy. The parameters used for the irradiation were ?? = 780nm, beam area 0.04 cm2, 40mW output power, energy density of 10 J / cm2. periods of 7 and 14 days were analyzed. To cause hypertrophy model was used for ablation of synergists being taken for this study, the gastrocnemius medial and lateral muscles and the soleus muscle, leaving the plantaris muscle suffered overload. the cross-sectional area were analyzed, collagen area, the number of myonuclei and mature blood vessels in muscle tissue and the organization and arrangement of collagen fibers in the tendon. For the experiment in vitro endothelial cells were used (HUVECs) were divided into control group and the other groups irradiated also with ?? = 780nm, 0.04 cm2 beam area, 40mW output power and energy density of 1 J / cm2, 5J / cm2, 10 J / cm2 to 20J / cm2. The different application time was calculated for all groups received the same dose of energy. They assessed cell viability, total protein and vessel formation in Matrigel. The in vivo results showed that there was increased cross-sectional area of the irradiated group after 14 days (26.3%) when compared to non-irradiated group. There was also increased number of myonuclei in the irradiated group after 14 days. The total area of muscle collagen increased (4.2%) in the irradiated group in 7 days and decreased (6.4%) in the irradiated group after 14 days when compared to non-irradiated group in both periods. Analysis by birefringence in the tendons showed better organization of the fibers 7 and 14 days when compared to non-irradiated group. In vitro results have shown decrease in cell viability in all irradiated all groups compared to the control and counting mature vessels was increased number of vessels in the irradiated group even after 14 days. In conclusion laser irradiation increased muscle mass, the number of myonuclei and the number of mature vessels and decreased collagen total area of muscle tissue after 14 days; improved tendon collagen organization for 7 and 14 days and interfere with the viability and total protein concentration in a dose-dependent endothelial cells HUVECs. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-06-28 |
dc.date.accessioned.fl_str_mv |
2018-06-14T20:50:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Terena, Stella Maris Lins. Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro. 2016. 69 f. Tese( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1776 |
identifier_str_mv |
Terena, Stella Maris Lins. Efeitos do LBP (780nm) sobre os aspectos morfol??gicos do complexo m??sculo tend??neo do m??sculo plantar de ratos durante o processo de hipertrofia compensat??ria: in vivo e in vitro. 2016. 69 f. Tese( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1776 |
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por |
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por |
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8765449414823306929 |
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600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
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Universidade Nove de Julho (UNINOVE) |
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UNINOVE |
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UNINOVE |
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Biblioteca Digital de Teses e Dissertações da Uninove |
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Biblioteca Digital de Teses e Dissertações da Uninove |
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Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
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1811016874174971904 |