Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/tede/handle/tede/901 |
Resumo: | The low level laser therapy (LLLT) is widely used in order to promote faster and better repair muscle with reference to different types of injury, however, studies on the prior application of the laser are scarce. The aim of this study is to evaluate the effect of LLLT prior to injury on the repair process and the matrix metalloproteinase MMP-2. For that, were used 40 Wistar rats divided into 5 groups: (G1) Control, (G2) Sham; (G3) No lesion with LLLT immediately prior to sacrifice; (G4) LLLT immediately prior to muscle injury TAD; (G5) Only injury without treatment with LLLT. The cryoinjury applications consist of two rod cooled in liquid nitrogen directly into the TA muscle. Groups 4 and 5 were assessed at 1, 3 and 7 days after injury. The LLLT were performed using the GaAlAs laser (?? 780nm) with the density energy of 10 J/cm??, output power of 40 mW, time of 10 seconds per point, with 8 points of application and total energy 3.2 J. After treatment, the TA muscles were removed and sections were obtained with the assistance of a microtome for morphology analysis using hematoxylin-eosin and collagen using Picrosirius staining. To assess the activity of MMP-2 is used technique Zymography in the different groups and its characterization technique was used for Western blotting. The results demonstrate that LBP applied prior to induction of the injury caused a reduction of inflammatory cells after 3 days, myonecrosis after 1 and 3 days, an increase of blood vessels after 3 and 7 days and after 7 days immature fibers. There was increased activity of MMP-2 after 7 days and reduced collagen deposition in the same period, with better organization of your arrangement tissue. In conclusion, LBP applied immediately before the injury induced positive effects on the modulation of inflammation and repair muscle activity of MMP-2 and organization and collagen deposition during muscle regeneration. |
id |
NOVE_c93166a68a218e57b20ecf4ec7080b89 |
---|---|
oai_identifier_str |
oai:localhost:tede/901 |
network_acronym_str |
NOVE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
repository_id_str |
|
spelling |
Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Nunes, Fabio Daumashttp://lattes.cnpq.br/4909755821591847Fernandes, Kristiane Santos PortaCPF:36124194805http://lattes.cnpq.br/2211419015181709Cardoso, Beatriz Guimar??es Ribeiro2015-04-22T17:20:45Z2014-12-022013-12-16CARDOSO, Beatriz Guimar??es Ribeiro. Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato. 2013. 59 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2013.http://bibliotecatede.uninove.br/tede/handle/tede/901The low level laser therapy (LLLT) is widely used in order to promote faster and better repair muscle with reference to different types of injury, however, studies on the prior application of the laser are scarce. The aim of this study is to evaluate the effect of LLLT prior to injury on the repair process and the matrix metalloproteinase MMP-2. For that, were used 40 Wistar rats divided into 5 groups: (G1) Control, (G2) Sham; (G3) No lesion with LLLT immediately prior to sacrifice; (G4) LLLT immediately prior to muscle injury TAD; (G5) Only injury without treatment with LLLT. The cryoinjury applications consist of two rod cooled in liquid nitrogen directly into the TA muscle. Groups 4 and 5 were assessed at 1, 3 and 7 days after injury. The LLLT were performed using the GaAlAs laser (?? 780nm) with the density energy of 10 J/cm??, output power of 40 mW, time of 10 seconds per point, with 8 points of application and total energy 3.2 J. After treatment, the TA muscles were removed and sections were obtained with the assistance of a microtome for morphology analysis using hematoxylin-eosin and collagen using Picrosirius staining. To assess the activity of MMP-2 is used technique Zymography in the different groups and its characterization technique was used for Western blotting. The results demonstrate that LBP applied prior to induction of the injury caused a reduction of inflammatory cells after 3 days, myonecrosis after 1 and 3 days, an increase of blood vessels after 3 and 7 days and after 7 days immature fibers. There was increased activity of MMP-2 after 7 days and reduced collagen deposition in the same period, with better organization of your arrangement tissue. In conclusion, LBP applied immediately before the injury induced positive effects on the modulation of inflammation and repair muscle activity of MMP-2 and organization and collagen deposition during muscle regeneration.O laser de baixa pot??ncia (LBP) tem sido utilizado por promover um reparo muscular de melhor qualidade, por??m, pouco se sabe sobre seus efeitos quando aplicado previamente. O objetivo do presente estudo foi avaliar os efeitos do LBP previamente a les??o muscular sobre o processo de reparo, atividade da metaloprotease de matriz MMP-2 e deposi????o do col??geno. Foram utilizados 40 ratos Wistar, divididos em 5 grupos: (G1) Controle; (G2) Sham; (G3) Somente irradia????o com laser; (G4) LBP previamente ?? les??o; (G5) Somente les??o. A crioles??o consistiu de duas aplica????es de bast??o resfriado em nitrog??nio l??quido no m??sculo tibial anterior (TA). Os grupos 4 e 5 foram avaliados em 1, 3 e 7 dias ap??s a les??o. A irradia????o pr??via foi realizada com o laser AsGaAl (?? 780nm; 10 J/cm??; 40 mW; 10 segundos por ponto; 8 pontos; 3.2 J) e ao t??rmino do protocolo, os m??sculos TA foram removidos para an??lise morfol??gica (colora????o com hematoxilina-eosina), col??geno (colora????o de Picrosirius) e atividade de MMP-2 pela t??cnica de Zimografia. Os resultados demonstraram que o LBP aplicado previamente a les??o reduziu o infiltrado inflamat??rio ap??s 3 dias, mionecrose ap??s 1 e 3 dias, aumentou o n??mero de vasos sangu??neos ap??s 3 e 7 dias e de fibras imaturas ap??s 7 dias. Tamb??m aumentou a atividade da MMP-2 e reduziu a deposi????o de col??geno ap??s 7 dias. Concluindo, o LBP previamente ?? les??o modulou positivamente o processo inflamat??rio e reparo muscular, atividade da MMP-2 e organiza????o e deposi????o do col??geno durante a regenera????o muscular.Made available in DSpace on 2015-04-22T17:20:45Z (GMT). No. of bitstreams: 1 Beatriz Guimaraes Ribeiro.pdf: 1897913 bytes, checksum: af303411aec302526518b9f8115fa451 (MD5) Previous issue date: 2013-12-16application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBRSa??deterapia a laser de baixa pot??nciam??sculo tibial anteriorregenera????olaser therapylow-levelmuscleskeletalregenerationCIENCIAS DA SAUDEEfeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de ratoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALBeatriz Guimaraes Ribeiro.pdfapplication/pdf1897913http://localhost:8080/tede/bitstream/tede/901/1/Beatriz+Guimaraes+Ribeiro.pdfaf303411aec302526518b9f8115fa451MD51tede/9012018-06-15 12:21:09.362oai:localhost:tede/901Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T15:21:09Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
title |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
spellingShingle |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato Cardoso, Beatriz Guimar??es Ribeiro terapia a laser de baixa pot??ncia m??sculo tibial anterior regenera????o laser therapy low-level muscle skeletal regeneration CIENCIAS DA SAUDE |
title_short |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
title_full |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
title_fullStr |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
title_full_unstemmed |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
title_sort |
Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato |
author |
Cardoso, Beatriz Guimar??es Ribeiro |
author_facet |
Cardoso, Beatriz Guimar??es Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7048141683554943 |
dc.contributor.referee1.fl_str_mv |
Nunes, Fabio Daumas |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4909755821591847 |
dc.contributor.referee2.fl_str_mv |
Fernandes, Kristiane Santos Porta |
dc.contributor.authorID.fl_str_mv |
CPF:36124194805 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2211419015181709 |
dc.contributor.author.fl_str_mv |
Cardoso, Beatriz Guimar??es Ribeiro |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Nunes, Fabio Daumas Fernandes, Kristiane Santos Porta |
dc.subject.por.fl_str_mv |
terapia a laser de baixa pot??ncia m??sculo tibial anterior regenera????o |
topic |
terapia a laser de baixa pot??ncia m??sculo tibial anterior regenera????o laser therapy low-level muscle skeletal regeneration CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
laser therapy low-level muscle skeletal regeneration |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The low level laser therapy (LLLT) is widely used in order to promote faster and better repair muscle with reference to different types of injury, however, studies on the prior application of the laser are scarce. The aim of this study is to evaluate the effect of LLLT prior to injury on the repair process and the matrix metalloproteinase MMP-2. For that, were used 40 Wistar rats divided into 5 groups: (G1) Control, (G2) Sham; (G3) No lesion with LLLT immediately prior to sacrifice; (G4) LLLT immediately prior to muscle injury TAD; (G5) Only injury without treatment with LLLT. The cryoinjury applications consist of two rod cooled in liquid nitrogen directly into the TA muscle. Groups 4 and 5 were assessed at 1, 3 and 7 days after injury. The LLLT were performed using the GaAlAs laser (?? 780nm) with the density energy of 10 J/cm??, output power of 40 mW, time of 10 seconds per point, with 8 points of application and total energy 3.2 J. After treatment, the TA muscles were removed and sections were obtained with the assistance of a microtome for morphology analysis using hematoxylin-eosin and collagen using Picrosirius staining. To assess the activity of MMP-2 is used technique Zymography in the different groups and its characterization technique was used for Western blotting. The results demonstrate that LBP applied prior to induction of the injury caused a reduction of inflammatory cells after 3 days, myonecrosis after 1 and 3 days, an increase of blood vessels after 3 and 7 days and after 7 days immature fibers. There was increased activity of MMP-2 after 7 days and reduced collagen deposition in the same period, with better organization of your arrangement tissue. In conclusion, LBP applied immediately before the injury induced positive effects on the modulation of inflammation and repair muscle activity of MMP-2 and organization and collagen deposition during muscle regeneration. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12-16 |
dc.date.available.fl_str_mv |
2014-12-02 |
dc.date.accessioned.fl_str_mv |
2015-04-22T17:20:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARDOSO, Beatriz Guimar??es Ribeiro. Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato. 2013. 59 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2013. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/tede/handle/tede/901 |
identifier_str_mv |
CARDOSO, Beatriz Guimar??es Ribeiro. Efeito da laserterapia pr??via sobre o reparo m??sculo esquel??tico de rato. 2013. 59 f. Disserta????o (Mestrado em Sa??de) - Universidade Nove de Julho, S??o Paulo, 2013. |
url |
http://bibliotecatede.uninove.br/tede/handle/tede/901 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
instname_str |
Universidade Nove de Julho (UNINOVE) |
instacron_str |
UNINOVE |
institution |
UNINOVE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/901/1/Beatriz+Guimaraes+Ribeiro.pdf |
bitstream.checksum.fl_str_mv |
af303411aec302526518b9f8115fa451 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
_version_ |
1800312346683375616 |