Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1789 |
Resumo: | The bone defects are caused by several factors and the repair process can be slow, where inflammatory and proliferative phases of remodeling of this tissue are extremely important for the quality of the repair. There are several therapies where the main is the surgery and in this case, the use of bone substitutes can be indicated. However the repair process may require assistance. Thus, the use of activated charcoal as bone substitute when the implementation of low-power laser to aid bone repair can be an alternative to these problems. The aim of this study was to verify the use of activated carbon felt as bone substitute and the interaction with the laser in the process of bone repair in rat tibias, assessing biochemical, histological and biomechanical changes. Material and methods: male wistar rats, 35 were used between 150 g to 200 g, with 3 months of age. The animals were anesthetized with ketamine hydrochloride and Association (90mg/Kg and 10mg/Kg, IP). Once anesthetized, the animals were put on surgical table, the skin was dissected and were made monocortical bone defects in the central region of the right tibia of mice. Immediately after surgery the rats were randomized and divided into the following groups: control (CTL), untreated Injury (NT), Lesion treated with activated carbon felt (AF), Lesion treated with lasertherapy (L6J) and Lesion treated with association of activated carbon felt and laser 830nm, 6J-100mW (AF+L). After 28 days the animals were euthanized with overdose of the same anesthetic, blood was collected and the tibia was taken for biochemical, histological and biomechanical analysis. Committee of ethics: AN00462014-UNINOVE. Results: the NT group reduction in bending forces and histological changes related to disorganization of the tissue. The group showed no L improve mechanical properties, whereas AF increase the strain in compare to CTL. AF+L group presented better flexion forces, related with improvement of histological aspects, however increased the alkaline phosphatase levels. Conclusion: the use of activated carbon felt seems to improve the bone repair induced in this study. The laser association with activated carbon felt showed improvement of biomechanical properties and still the histological results have a better aspect. |
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Marcos, Rodrigo LabatMarcos, Rodrigo LabatLeal Junior, Ernesto Cesar PintoCarvalho, Rodrigo Leal de Paivahttp://lattes.cnpq.br/5598003035609502Foster, Fernando Gredinare2018-06-18T19:03:08Z2016-12-17Foster, Fernando Gredinare. Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos. 2016. 82 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1789The bone defects are caused by several factors and the repair process can be slow, where inflammatory and proliferative phases of remodeling of this tissue are extremely important for the quality of the repair. There are several therapies where the main is the surgery and in this case, the use of bone substitutes can be indicated. However the repair process may require assistance. Thus, the use of activated charcoal as bone substitute when the implementation of low-power laser to aid bone repair can be an alternative to these problems. The aim of this study was to verify the use of activated carbon felt as bone substitute and the interaction with the laser in the process of bone repair in rat tibias, assessing biochemical, histological and biomechanical changes. Material and methods: male wistar rats, 35 were used between 150 g to 200 g, with 3 months of age. The animals were anesthetized with ketamine hydrochloride and Association (90mg/Kg and 10mg/Kg, IP). Once anesthetized, the animals were put on surgical table, the skin was dissected and were made monocortical bone defects in the central region of the right tibia of mice. Immediately after surgery the rats were randomized and divided into the following groups: control (CTL), untreated Injury (NT), Lesion treated with activated carbon felt (AF), Lesion treated with lasertherapy (L6J) and Lesion treated with association of activated carbon felt and laser 830nm, 6J-100mW (AF+L). After 28 days the animals were euthanized with overdose of the same anesthetic, blood was collected and the tibia was taken for biochemical, histological and biomechanical analysis. Committee of ethics: AN00462014-UNINOVE. Results: the NT group reduction in bending forces and histological changes related to disorganization of the tissue. The group showed no L improve mechanical properties, whereas AF increase the strain in compare to CTL. AF+L group presented better flexion forces, related with improvement of histological aspects, however increased the alkaline phosphatase levels. Conclusion: the use of activated carbon felt seems to improve the bone repair induced in this study. The laser association with activated carbon felt showed improvement of biomechanical properties and still the histological results have a better aspect.Les??es ??sseas s??o dif??ceis de serem tratadas e dependendo do grau da les??o, a interven????o cir??rgica ?? um dos tratamentos utilizados. Neste caso, o uso de substitutos ??sseos pode ser necess??rio. A terapia com laser de baixa pot??ncia tqmb??m ?? indicada como terapia auxiliar, pela sua importante a????o analg??sica e de reparo. Por??m, pouco se sabe a respeito da intera????o entre biomateriais e laserterapia. O objetivo deste trabalho foi verificar o uso do feltro ativado como substituto ??sseo e a intera????o com a laserterapia de baixa pot??ncia no processo de reparo ??sseo em t??bias de ratos, analisando aspectos histol??gicos, bioqu??micos e biomec??nicos. Material e M??todos: Foram utilizados 35 ratos wistar, entre 150g ?? 200g, com 3 meses de idade. Depois de anestesiados, os animais foram colocados em mesa cir??rgica, a pele foi dissecada e o defeito ??sseo monocortical foi confeccionado na regi??o central da t??bia direita dos animais. Imediatamente ap??s a cirurgia os ratos foram randomizados e organizados nos seguintes grupos: Controle (CTL), Les??o n??o tratado (NT), Les??o tratado com feltro ativado (AF), Les??o tratado com Laser (L6J) e Les??o tratado com associa????o do feltro ativado e laser 830nm, 6J ??? 100mW (AF+L). Ap??s 28 dias os animais foram eutanasiados, o sangue foi coletado e a t??bia removida para an??lises histol??gicas e biomec??nicas. Resultados: O grupo NT apresentou redu????o do reparo ??sseo traduzido pela baixa organiza????o histol??gica e redu????o da resist??ncia mec??nica. O grupo AF apresentou aumento da capacidade de deforma????o enquanto que o grupo L apresentou melhor organiza????o histol??gica. O grupo AF+L apresentou melhora na resist??ncia mec??nica. Conclu??mos que o laser associado ao uso do feltro de carbono ativado auxiliou o reparo ??sseo no modelo de les??o em t??bia de ratos.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-18T19:03:08Z No. of bitstreams: 1 Fernando Gredinare Foster.pdf: 984670 bytes, checksum: afea175c4e00ac9803bd6bdda1570d7d (MD5)Made available in DSpace on 2018-06-18T19:03:08Z (GMT). No. of bitstreams: 1 Fernando Gredinare Foster.pdf: 984670 bytes, checksum: afea175c4e00ac9803bd6bdda1570d7d (MD5) Previous issue date: 2016-12-17application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??deUNINOVEBrasilSa??dereparo ??sseolaser de baixa pot??nciafeltro ativadopropriedades mec??nicasbone repairlow level laseractivated carbon feltmechanical propertiesCIENCIAS DA SAUDEEfeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALFernando Gredinare Foster.pdfFernando Gredinare Foster.pdfapplication/pdf984670http://localhost:8080/tede/bitstream/tede/1789/2/Fernando+Gredinare+Foster.pdfafea175c4e00ac9803bd6bdda1570d7dMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1789/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/17892018-06-18 16:03:08.199oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-18T19:03:08Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
title |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
spellingShingle |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos Foster, Fernando Gredinare reparo ??sseo laser de baixa pot??ncia feltro ativado propriedades mec??nicas bone repair low level laser activated carbon felt mechanical properties CIENCIAS DA SAUDE |
title_short |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
title_full |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
title_fullStr |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
title_full_unstemmed |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
title_sort |
Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos |
author |
Foster, Fernando Gredinare |
author_facet |
Foster, Fernando Gredinare |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee1.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee2.fl_str_mv |
Leal Junior, Ernesto Cesar Pinto |
dc.contributor.referee3.fl_str_mv |
Carvalho, Rodrigo Leal de Paiva |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5598003035609502 |
dc.contributor.author.fl_str_mv |
Foster, Fernando Gredinare |
contributor_str_mv |
Marcos, Rodrigo Labat Marcos, Rodrigo Labat Leal Junior, Ernesto Cesar Pinto Carvalho, Rodrigo Leal de Paiva |
dc.subject.por.fl_str_mv |
reparo ??sseo laser de baixa pot??ncia feltro ativado propriedades mec??nicas |
topic |
reparo ??sseo laser de baixa pot??ncia feltro ativado propriedades mec??nicas bone repair low level laser activated carbon felt mechanical properties CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
bone repair low level laser activated carbon felt mechanical properties |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The bone defects are caused by several factors and the repair process can be slow, where inflammatory and proliferative phases of remodeling of this tissue are extremely important for the quality of the repair. There are several therapies where the main is the surgery and in this case, the use of bone substitutes can be indicated. However the repair process may require assistance. Thus, the use of activated charcoal as bone substitute when the implementation of low-power laser to aid bone repair can be an alternative to these problems. The aim of this study was to verify the use of activated carbon felt as bone substitute and the interaction with the laser in the process of bone repair in rat tibias, assessing biochemical, histological and biomechanical changes. Material and methods: male wistar rats, 35 were used between 150 g to 200 g, with 3 months of age. The animals were anesthetized with ketamine hydrochloride and Association (90mg/Kg and 10mg/Kg, IP). Once anesthetized, the animals were put on surgical table, the skin was dissected and were made monocortical bone defects in the central region of the right tibia of mice. Immediately after surgery the rats were randomized and divided into the following groups: control (CTL), untreated Injury (NT), Lesion treated with activated carbon felt (AF), Lesion treated with lasertherapy (L6J) and Lesion treated with association of activated carbon felt and laser 830nm, 6J-100mW (AF+L). After 28 days the animals were euthanized with overdose of the same anesthetic, blood was collected and the tibia was taken for biochemical, histological and biomechanical analysis. Committee of ethics: AN00462014-UNINOVE. Results: the NT group reduction in bending forces and histological changes related to disorganization of the tissue. The group showed no L improve mechanical properties, whereas AF increase the strain in compare to CTL. AF+L group presented better flexion forces, related with improvement of histological aspects, however increased the alkaline phosphatase levels. Conclusion: the use of activated carbon felt seems to improve the bone repair induced in this study. The laser association with activated carbon felt showed improvement of biomechanical properties and still the histological results have a better aspect. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-17 |
dc.date.accessioned.fl_str_mv |
2018-06-18T19:03:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Foster, Fernando Gredinare. Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos. 2016. 82 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1789 |
identifier_str_mv |
Foster, Fernando Gredinare. Efeito do laser de baixa pot??ncia associado ao uso de feltro ativado no processo de reparo ??sseo em t??bia de ratos. 2016. 82 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1789 |
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por |
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por |
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8765449414823306929 |
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openAccess |
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Universidade Nove de Julho |
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Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de |
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UNINOVE |
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Brasil |
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Sa??de |
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Universidade Nove de Julho |
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