Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato

Detalhes bibliográficos
Autor(a) principal: Pavesi, Vanessa Christina Santos
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/tede/handle/tede/796
Resumo: The aim of the present study was to investigate components of extracellular matrix (ECM) during the skeletal muscle regeneration after cryolesion in rats. Sixty adult Wistar rats were used. The animals were divided into 4 groups: 1, 7, 14 and 21 days post-injury (10animls/group). One of the tibialis anterior muscles was cryolesioned and the contralateral muscle was used as the control. One sham group, in which animals received only the surgical procedure and one group of control animals that do not receive any intervention were included. The muscles were submitted to hematoxylin-eosin and immunohistochemistry staining for collagen IV, MMP-2 and MMP-9 detection. The MMP enzymatic activity was verified by zymography. The control muscles showed normal morphology and the sham group exhibited an inflammatory infiltrated. After 1 day, the cryolesioned group exhibit moderate inflammatory infiltrated and necrosis areas. At 7 and 14 days a significant reduction of the inflammatory process, absence of necrosis and presence of immature muscular cells could be observed. The injured group revealed total remodeling after the 21 days. Collagen IV showed a disordered distribution pattern after 1 day of injury and gradually it was reorganized during the 21 days of the muscle regeneration. The MMP-2 and MMP-9 immunolabeling, as well, the MMP-2 activity were related to the phase of skeletal muscle repair. After 1 day, an increase on MMP-2 and MMP-9 labeling and MMP-2 activity were observed. In conclusion, the increase and decrease of immunolabeling and activity of MMP were related to the break down and accumulation of collagen IV during the skeletal muscle remodeling.
id NOVE_28f0837fb3174515e4c5757aa69e032b
oai_identifier_str oai:localhost:tede/796
network_acronym_str NOVE
network_name_str Biblioteca Digital de Teses e Dissertações da Uninove
repository_id_str
spelling Martins, Manoela DominguesCPF:00000000000http://lattes.cnpq.br/4958184722611235Ferrari, Raquel Agnelli Mesquitahttp://lattes.cnpq.br/7048141683554943Silva, Carlos GilbertoCPF:00000000000Miyabara, HelenCPF:00000000000CPF:16298081801http://lattes.cnpq.br/9744958700550564Pavesi, Vanessa Christina Santos2015-04-22T17:20:18Z2013-04-242008-12-10PAVESI, Vanessa Christina Santos. Effect of cryoinjury in extracellular matrix remodeling in rat skeletal muscle. 2008. 82 f. Dissertação (Mestrado em Saúde) - Universidade Nove de Julho, São Paulo, 2008.http://bibliotecatede.uninove.br/tede/handle/tede/796The aim of the present study was to investigate components of extracellular matrix (ECM) during the skeletal muscle regeneration after cryolesion in rats. Sixty adult Wistar rats were used. The animals were divided into 4 groups: 1, 7, 14 and 21 days post-injury (10animls/group). One of the tibialis anterior muscles was cryolesioned and the contralateral muscle was used as the control. One sham group, in which animals received only the surgical procedure and one group of control animals that do not receive any intervention were included. The muscles were submitted to hematoxylin-eosin and immunohistochemistry staining for collagen IV, MMP-2 and MMP-9 detection. The MMP enzymatic activity was verified by zymography. The control muscles showed normal morphology and the sham group exhibited an inflammatory infiltrated. After 1 day, the cryolesioned group exhibit moderate inflammatory infiltrated and necrosis areas. At 7 and 14 days a significant reduction of the inflammatory process, absence of necrosis and presence of immature muscular cells could be observed. The injured group revealed total remodeling after the 21 days. Collagen IV showed a disordered distribution pattern after 1 day of injury and gradually it was reorganized during the 21 days of the muscle regeneration. The MMP-2 and MMP-9 immunolabeling, as well, the MMP-2 activity were related to the phase of skeletal muscle repair. After 1 day, an increase on MMP-2 and MMP-9 labeling and MMP-2 activity were observed. In conclusion, the increase and decrease of immunolabeling and activity of MMP were related to the break down and accumulation of collagen IV during the skeletal muscle remodeling.O objetivo deste estudo foi investigar os componentes da matriz extracelular (MEC) durante a regeneração muscular esquelética. Foram utilizados 60 ratos Wistar sendo que destes, foram organizados 4 grupos avaliados 1, 7, 14 e 21 dias após a lesão (10 animais/grupo). Esses grupos foram submetidos à criolesão no músculo tibial anterior do lado esquerdo e os músculos contralaterais foram os controles. Foi realizado um grupo sham, que recebeu apenas o procedimento cirúrgico e um grupo de animais controle que não recebeu nenhuma intervenção. Os músculos foram submetidos à colorações por hematoxilina & eosina e imuno-histoquímica para detecção de colágeno tipo IV, MMP-2 e MMP-9. A atividade enzimática de MMPs foi verificada pela zimografia. Os músculos controle mostraram morfologia normal, o grupo sham exibiu leve infiltrado inflamatório. No grupo criolesionado foi observado após 1 dia moderado infiltrado inflamatório e áreas de necrose. Após 7 e 14 dias pode-se observar redução significativa do processo inflamatório, ausência de necrose e presença de células musculares imaturas. Aos 21 dias o músculo mostrou evidência de reparo completo. O colágeno IV mostrou-se desorganizado por entre as fibras lesadas após 1 dia da criolesão e se reorganizou gradualmente ao longo dos 21 dias. A imunoreatividade da MMP-2 e MMP-9, assim como a atividade de MMP-2 se mostraram relacionadas com a fase do reparo tecidual. Após 1 dia da injúria foi observado aumento da imunomarcação da MMP-2 e MMP-9 e da atividade da MMP-2. Conclui-se que, aumento e diminuição dos níveis de marcação e atividade de MMPs estão relacionados com a degradação e o acúmulo de colágeno IV, durante o remodelamento da MEC no músculo esquelético.Made available in DSpace on 2015-04-22T17:20:18Z (GMT). No. of bitstreams: 1 B_Vanessa Christina Santos Pavesi.pdf: 907129 bytes, checksum: 86f0acb23fd1cc1a96b220e4648b16a8 (MD5) Previous issue date: 2008-12-10application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Ciências da ReabilitaçãoUNINOVEBRSaúdeRemodelamento MuscularMatriz ExtracelularColágeno IVMMP-2, MMP-9Muscle RemodelingExtracellular MatrixCollagen IVMMP-2, MMP-9CIENCIAS DA SAUDEEfeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de ratoEffect of cryoinjury in extracellular matrix remodeling in rat skeletal muscleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALB_Vanessa Christina Santos Pavesi.pdfapplication/pdf907129http://localhost:8080/tede/bitstream/tede/796/1/B_Vanessa+Christina+Santos+Pavesi.pdf86f0acb23fd1cc1a96b220e4648b16a8MD51tede/7962022-06-02 15:11:51.199oai:localhost:tede/796Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-06-02T18:11:51Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
dc.title.alternative.eng.fl_str_mv Effect of cryoinjury in extracellular matrix remodeling in rat skeletal muscle
title Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
spellingShingle Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
Pavesi, Vanessa Christina Santos
Remodelamento Muscular
Matriz Extracelular
Colágeno IV
MMP-2, MMP-9
Muscle Remodeling
Extracellular Matrix
Collagen IV
MMP-2, MMP-9
CIENCIAS DA SAUDE
title_short Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
title_full Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
title_fullStr Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
title_full_unstemmed Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
title_sort Efeito da criolesão no remodelamento da matriz extracelular em músculo esquelético de rato
author Pavesi, Vanessa Christina Santos
author_facet Pavesi, Vanessa Christina Santos
author_role author
dc.contributor.advisor1.fl_str_mv Martins, Manoela Domingues
dc.contributor.advisor1ID.fl_str_mv CPF:00000000000
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4958184722611235
dc.contributor.advisor-co1.fl_str_mv Ferrari, Raquel Agnelli Mesquita
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/7048141683554943
dc.contributor.referee1.fl_str_mv Silva, Carlos Gilberto
dc.contributor.referee1ID.fl_str_mv CPF:00000000000
dc.contributor.referee2.fl_str_mv Miyabara, Helen
dc.contributor.referee2ID.fl_str_mv CPF:00000000000
dc.contributor.authorID.fl_str_mv CPF:16298081801
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9744958700550564
dc.contributor.author.fl_str_mv Pavesi, Vanessa Christina Santos
contributor_str_mv Martins, Manoela Domingues
Ferrari, Raquel Agnelli Mesquita
Silva, Carlos Gilberto
Miyabara, Helen
dc.subject.por.fl_str_mv Remodelamento Muscular
Matriz Extracelular
Colágeno IV
MMP-2, MMP-9
topic Remodelamento Muscular
Matriz Extracelular
Colágeno IV
MMP-2, MMP-9
Muscle Remodeling
Extracellular Matrix
Collagen IV
MMP-2, MMP-9
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv Muscle Remodeling
Extracellular Matrix
Collagen IV
MMP-2, MMP-9
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The aim of the present study was to investigate components of extracellular matrix (ECM) during the skeletal muscle regeneration after cryolesion in rats. Sixty adult Wistar rats were used. The animals were divided into 4 groups: 1, 7, 14 and 21 days post-injury (10animls/group). One of the tibialis anterior muscles was cryolesioned and the contralateral muscle was used as the control. One sham group, in which animals received only the surgical procedure and one group of control animals that do not receive any intervention were included. The muscles were submitted to hematoxylin-eosin and immunohistochemistry staining for collagen IV, MMP-2 and MMP-9 detection. The MMP enzymatic activity was verified by zymography. The control muscles showed normal morphology and the sham group exhibited an inflammatory infiltrated. After 1 day, the cryolesioned group exhibit moderate inflammatory infiltrated and necrosis areas. At 7 and 14 days a significant reduction of the inflammatory process, absence of necrosis and presence of immature muscular cells could be observed. The injured group revealed total remodeling after the 21 days. Collagen IV showed a disordered distribution pattern after 1 day of injury and gradually it was reorganized during the 21 days of the muscle regeneration. The MMP-2 and MMP-9 immunolabeling, as well, the MMP-2 activity were related to the phase of skeletal muscle repair. After 1 day, an increase on MMP-2 and MMP-9 labeling and MMP-2 activity were observed. In conclusion, the increase and decrease of immunolabeling and activity of MMP were related to the break down and accumulation of collagen IV during the skeletal muscle remodeling.
publishDate 2008
dc.date.issued.fl_str_mv 2008-12-10
dc.date.available.fl_str_mv 2013-04-24
dc.date.accessioned.fl_str_mv 2015-04-22T17:20:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAVESI, Vanessa Christina Santos. Effect of cryoinjury in extracellular matrix remodeling in rat skeletal muscle. 2008. 82 f. Dissertação (Mestrado em Saúde) - Universidade Nove de Julho, São Paulo, 2008.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/tede/handle/tede/796
identifier_str_mv PAVESI, Vanessa Christina Santos. Effect of cryoinjury in extracellular matrix remodeling in rat skeletal muscle. 2008. 82 f. Dissertação (Mestrado em Saúde) - Universidade Nove de Julho, São Paulo, 2008.
url http://bibliotecatede.uninove.br/tede/handle/tede/796
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
dc.relation.confidence.fl_str_mv 600
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências da Reabilitação
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Saúde
publisher.none.fl_str_mv Universidade Nove de Julho
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da Uninove
instname:Universidade Nove de Julho (UNINOVE)
instacron:UNINOVE
instname_str Universidade Nove de Julho (UNINOVE)
instacron_str UNINOVE
institution UNINOVE
reponame_str Biblioteca Digital de Teses e Dissertações da Uninove
collection Biblioteca Digital de Teses e Dissertações da Uninove
bitstream.url.fl_str_mv http://localhost:8080/tede/bitstream/tede/796/1/B_Vanessa+Christina+Santos+Pavesi.pdf
bitstream.checksum.fl_str_mv 86f0acb23fd1cc1a96b220e4648b16a8
bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)
repository.mail.fl_str_mv bibliotecatede@uninove.br||bibliotecatede@uninove.br
_version_ 1811016863019171840