Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/2845 |
Resumo: | Phototherapy has been widely used in the treatment of osteo-degenerative diseases, with the objective of evaluating its effects on bone metabolism. Clinical observations have suggested that low intensity light therapy has beneficial effects on the bone healing process. Although specific clinical protocols have not yet been established, several authors have reported photobiomodulatory effects on healing processes in animal models and tissue culture media. Little is known about the effects of different spectra on the viability and differentiation of rat calvarial osteoblasts (ÓSTEO 1). Therefore, the objective of this work was to compare the influence of phototherapy with LBI, LED's and Magneto on the viability and differentiation of osteoblasts from calvaria of rats (ostreat 1). For this study the osteoblasts were cultured in 10% DMEM and 5% fetal bovine serum (FBS) culture and incubated in a CO2 oven. The cells were irradiated with 660 nm (red), 808 nm (infrared), 660 nm (red) LEDs, 850 nm (infrared) at 40 mW at 1.5 and 10 J / cm² . After cell adhesion, we used the magneto equipment with frequency of 0, 9 and 18 Hertz, at exposure times of 25, 125 and 250 seconds. 24 hours after irradiation, the viability analysis wasperformed by MTT and Violet Crystal assay in which the results suggest a partial increase of viable cells when stimulated by different spectra. For the cell differentiation assays, the activity of alkaline phosphatase was increased with Magneto at 9 hertz (250 seconds), Red Laser at 250 seconds and Infrared at 125 seconds in relation to the control group. After the phototherapy effect, the total protein activation analysis was performed, in which no differences were observed for any of the groups studied. In this way the Laser phototherapy, LED and Magneto application, the partial modulation of the osteoblasts was observed in relation to the viability, But in the process of differentiation of alkaline phos- phase activity there were few alterations. |
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Deana, Alessandro Melode, Alessandro MeloFerrari, Raquel Agnelli MesquitaMarcos, Rodrigo LabatDellê, HumbertoNavarro, Ricardo ScarparoSouza, Ana Maria de2022-04-08T20:19:40Z2016-12-07Souza, Ana Maria de. Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos. 2016. 56 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2845Phototherapy has been widely used in the treatment of osteo-degenerative diseases, with the objective of evaluating its effects on bone metabolism. Clinical observations have suggested that low intensity light therapy has beneficial effects on the bone healing process. Although specific clinical protocols have not yet been established, several authors have reported photobiomodulatory effects on healing processes in animal models and tissue culture media. Little is known about the effects of different spectra on the viability and differentiation of rat calvarial osteoblasts (ÓSTEO 1). Therefore, the objective of this work was to compare the influence of phototherapy with LBI, LED's and Magneto on the viability and differentiation of osteoblasts from calvaria of rats (ostreat 1). For this study the osteoblasts were cultured in 10% DMEM and 5% fetal bovine serum (FBS) culture and incubated in a CO2 oven. The cells were irradiated with 660 nm (red), 808 nm (infrared), 660 nm (red) LEDs, 850 nm (infrared) at 40 mW at 1.5 and 10 J / cm² . After cell adhesion, we used the magneto equipment with frequency of 0, 9 and 18 Hertz, at exposure times of 25, 125 and 250 seconds. 24 hours after irradiation, the viability analysis wasperformed by MTT and Violet Crystal assay in which the results suggest a partial increase of viable cells when stimulated by different spectra. For the cell differentiation assays, the activity of alkaline phosphatase was increased with Magneto at 9 hertz (250 seconds), Red Laser at 250 seconds and Infrared at 125 seconds in relation to the control group. After the phototherapy effect, the total protein activation analysis was performed, in which no differences were observed for any of the groups studied. In this way the Laser phototherapy, LED and Magneto application, the partial modulation of the osteoblasts was observed in relation to the viability, But in the process of differentiation of alkaline phos- phase activity there were few alterations.A fototerapia tem sido muito utilizada no tratamento de doenças osteo- degenerativas, com o objetivo de avaliar seus efeitos no metabolismo ósseo. Observações clínicas têm sugerido que a terapia com luz de baixa intensidade tenha efeitos benéficos no processo de consolidação óssea. Embora ainda não tenham sido estabelecidos protocolos clínicos específicos, vários autores têm reportado os efeitos fotobiomoduladores nos processos de cicatrização em modelos animais e em meios de cultura tecidual. Pouco se sabe sobre os efeitos de diferentes campos eletromagnéticos na viabilidade e diferenciação de osteoblastos de calvárias de rato (ÓSTEO 1). Diante disso, o objetivo deste trabalho foi avaliar de forma comparativa a influência da fototerapia com LBI, LED´s e Magneto na viabilidade e diferenciação de osteoblastos de calvária de ratos (òsteo 1). Para este estudo os osteoblastos foram cultivados a em meio de cultura DMEM a 10% e 5% de soro fetal bovino (SFB) e encubados em estufa de CO2. As células foram irradiadas pontualmente com os laseres 660 nm (vermellho), 808 nm (infravermelho), LED´s 660nm (vermelho), 850 nm (infravermelho) na potência de 40 mW nas doses de 1,5, e10 J/cm². Após a adesão celular, utilizamos o equipamento magneto com frequência de 0, 9 e 18 Hertz, nos tempos de exposição de 25, 125 e 250 segundos. 24 horas após a irradiação foi realizada a análise de viabilidade por meio de ensaio de MTT e Cristal Violeta nos quais os resultados sugerem aumento parcial de células viáveis quando estimuladas por diferentes campos. Para os ensaios de diferenciação celular foi realizada a atividade de fosfatase alcalina onde houve aumento com Magneto a 9 hertz (250 segundos), Laser vermelho a 250 segundos e LED infravermelho a 125 segundos em relação ao grupo controle. Após o efeito da fototerapia foi realizada a análise da concentração de proteínas totais onde não foram observadas diferenças para nenhum dos grupos estudados. Dessa forma a fototerapia Laser, LED e aplicação do Magneto, observou-se a modulação parcial dos osteoblastos em relação à viabilidade, porém no processo de diferenciação da atividade de fostase alcalina houve poucas alterações.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-08T20:19:40Z No. of bitstreams: 1 Ana Maria de Souza.pdf: 1446323 bytes, checksum: b461ae0d1a788fc2dbc272bf117fb3e4 (MD5)Made available in DSpace on 2022-04-08T20:19:40Z (GMT). No. of bitstreams: 1 Ana Maria de Souza.pdf: 1446323 bytes, checksum: b461ae0d1a788fc2dbc272bf117fb3e4 (MD5) Previous issue date: 2016-12-07application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdefototerapiaLLLTLEDmagnetoosteoblastosphototherapyLLLTLEDmagnetoosteoblastsCIENCIAS DA SAUDEEstudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastosComparative study of different electromagnetic fields on proliferation, viability, protein quantification and osteoblast differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALAna Maria de Souza.pdfAna Maria de Souza.pdfapplication/pdf1446323http://localhost:8080/tede/bitstream/tede/2845/2/Ana+Maria+de+Souza.pdfb461ae0d1a788fc2dbc272bf117fb3e4MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2845/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/28452022-04-08 17:19:40.237oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-04-08T20:19:40Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
dc.title.alternative.eng.fl_str_mv |
Comparative study of different electromagnetic fields on proliferation, viability, protein quantification and osteoblast differentiation |
title |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
spellingShingle |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos Souza, Ana Maria de fototerapia LLLT LED magneto osteoblastos phototherapy LLLT LED magneto osteoblasts CIENCIAS DA SAUDE |
title_short |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
title_full |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
title_fullStr |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
title_full_unstemmed |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
title_sort |
Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos |
author |
Souza, Ana Maria de |
author_facet |
Souza, Ana Maria de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Deana, Alessandro Melo |
dc.contributor.referee1.fl_str_mv |
de, Alessandro Melo |
dc.contributor.referee2.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee3.fl_str_mv |
Marcos, Rodrigo Labat |
dc.contributor.referee4.fl_str_mv |
Dellê, Humberto |
dc.contributor.referee5.fl_str_mv |
Navarro, Ricardo Scarparo |
dc.contributor.author.fl_str_mv |
Souza, Ana Maria de |
contributor_str_mv |
Deana, Alessandro Melo de, Alessandro Melo Ferrari, Raquel Agnelli Mesquita Marcos, Rodrigo Labat Dellê, Humberto Navarro, Ricardo Scarparo |
dc.subject.por.fl_str_mv |
fototerapia LLLT LED magneto osteoblastos phototherapy LLLT LED magneto osteoblasts |
topic |
fototerapia LLLT LED magneto osteoblastos phototherapy LLLT LED magneto osteoblasts CIENCIAS DA SAUDE |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Phototherapy has been widely used in the treatment of osteo-degenerative diseases, with the objective of evaluating its effects on bone metabolism. Clinical observations have suggested that low intensity light therapy has beneficial effects on the bone healing process. Although specific clinical protocols have not yet been established, several authors have reported photobiomodulatory effects on healing processes in animal models and tissue culture media. Little is known about the effects of different spectra on the viability and differentiation of rat calvarial osteoblasts (ÓSTEO 1). Therefore, the objective of this work was to compare the influence of phototherapy with LBI, LED's and Magneto on the viability and differentiation of osteoblasts from calvaria of rats (ostreat 1). For this study the osteoblasts were cultured in 10% DMEM and 5% fetal bovine serum (FBS) culture and incubated in a CO2 oven. The cells were irradiated with 660 nm (red), 808 nm (infrared), 660 nm (red) LEDs, 850 nm (infrared) at 40 mW at 1.5 and 10 J / cm² . After cell adhesion, we used the magneto equipment with frequency of 0, 9 and 18 Hertz, at exposure times of 25, 125 and 250 seconds. 24 hours after irradiation, the viability analysis wasperformed by MTT and Violet Crystal assay in which the results suggest a partial increase of viable cells when stimulated by different spectra. For the cell differentiation assays, the activity of alkaline phosphatase was increased with Magneto at 9 hertz (250 seconds), Red Laser at 250 seconds and Infrared at 125 seconds in relation to the control group. After the phototherapy effect, the total protein activation analysis was performed, in which no differences were observed for any of the groups studied. In this way the Laser phototherapy, LED and Magneto application, the partial modulation of the osteoblasts was observed in relation to the viability, But in the process of differentiation of alkaline phos- phase activity there were few alterations. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-07 |
dc.date.accessioned.fl_str_mv |
2022-04-08T20:19:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Souza, Ana Maria de. Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos. 2016. 56 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2845 |
identifier_str_mv |
Souza, Ana Maria de. Estudo comparativo de diferentes campos eletromagnéticos na proliferação, viabilidade, quantificação de proteínas e diferenciação de osteoblastos. 2016. 56 f. Tese( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/2845 |
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por |
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por |
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8765449414823306929 |
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Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde |
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UNINOVE |
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Brasil |
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Saúde |
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Universidade Nove de Julho |
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