Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios

Detalhes bibliográficos
Autor(a) principal: Silva, Carla Helrigle
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/1141
Resumo: The aim of this experimental study was to investigate the effects of LED phototherapy on the inflammation process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of inflammatory mediators IL-6, IL-10 and TNF-??. Thirty-five female Wistar rats were divided into 4 groups: 3 ovariectomized groups, untreated (NTTA); treated (TTA) and control subdivided into experimental periods of 3 , 7 and 14 days, totaling 30 rats and one group (n = 5) not ovariectomized (not OVX). Tendinitis was induced by trauma using a 208g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 hours after tendonitis with LED therapy ( 945nm , power 32mW, energy of 3.84 J, power density of 0.06 W / cm ??, energy density of 7.68 J / cm ??, beam output area of 0.5 cm ?? and time of 120 s) and every 48 hours at 3, 7 and 14 days. Then, euthanasia was performed in a CO2 chamber 24h after the last treatment. The tendons were dissected and stored in liquid nitrogen at -196??C, thawed only at the time of immunoenzymatic test (ELISA). After analysis of results, groups treated with LED showed decrease in the number of pro-inflammatory cells, IL- 6 and TNF- ?? (p < 0.05) and increased number of anti-inflammatory IL -10 ( p <0.05) even when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED in the studied parameters has repairing effect on the tendinopathy process (tendonitis) caused by trauma in female rats (OVX).
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spelling Carvalho, Regiane Albertini dehttp://lattes.cnpq.br/2515657728339113Costa, Maricilia Silvahttp://lattes.cnpq.br/7963390052508784Leal Junior, Ernesto Cesar Pintohttp://lattes.cnpq.br/816662648620111600003526160http://lattes.cnpq.br/3362144866086155Silva, Carla Helrigle2015-07-21T15:29:23Z2013-09-09Silva, Carla Helrigle. Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios. 2013. 54 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo .http://bibliotecatede.uninove.br/handle/tede/1141The aim of this experimental study was to investigate the effects of LED phototherapy on the inflammation process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of inflammatory mediators IL-6, IL-10 and TNF-??. Thirty-five female Wistar rats were divided into 4 groups: 3 ovariectomized groups, untreated (NTTA); treated (TTA) and control subdivided into experimental periods of 3 , 7 and 14 days, totaling 30 rats and one group (n = 5) not ovariectomized (not OVX). Tendinitis was induced by trauma using a 208g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 hours after tendonitis with LED therapy ( 945nm , power 32mW, energy of 3.84 J, power density of 0.06 W / cm ??, energy density of 7.68 J / cm ??, beam output area of 0.5 cm ?? and time of 120 s) and every 48 hours at 3, 7 and 14 days. Then, euthanasia was performed in a CO2 chamber 24h after the last treatment. The tendons were dissected and stored in liquid nitrogen at -196??C, thawed only at the time of immunoenzymatic test (ELISA). After analysis of results, groups treated with LED showed decrease in the number of pro-inflammatory cells, IL- 6 and TNF- ?? (p < 0.05) and increased number of anti-inflammatory IL -10 ( p <0.05) even when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED in the studied parameters has repairing effect on the tendinopathy process (tendonitis) caused by trauma in female rats (OVX).Este estudo experimental tem objetivo de analisar os efeitos da fototerapia LED, no processo inflamat??rio em tend??o de Aquiles de ratas ovariectomizadas (OVX), pelo envolvimento dos mediadores inflamat??rios IL-6, IL-10 e TNF-??. Foram utilizados 35 ratas Wistar f??meas, divididas em 4 grupos: sendo 3 grupos ovariectomizados, n??o tratados (NTTA); tratados (TTA) e controle subdivididos nos per??odos experimentais de 3, 7 e 14 dias, totalizando 30 ratos; e um grupo (n=5) n??o ovariectomizados (N??o OVX). A tendinite foi induzida por trauma, utilizando uma massa de 208g posicionada a 20 cm do tend??o direito de cada animal, com energia de 0,70J. Os animais foram tratados 12h ap??s a tendinite, com terapia LED (945nm, pot??ncia de 32mW, energia de 3,84J,densidade de pot??ncia 0,06W/cm??,densidade de energia 7,68J/cm??,??rea de sa??da do feixe 0,5 cm?? e tempo de 120s) e a cada 48h nos per??odos de 3 , 7 e 14 dias. Em seguida, a eutan??sia decorreu em c??mara de CO2, 24h ao ??ltimo tratamento. Os tend??es foram dissecados e acondicionados em nitrog??nio l??quido a 196??C negativos, descongelados apenas no momento da realiza????o do teste imuenzim??tioco (ELISA). Ap??s a analise dos resultados os grupos tratados com LED apresentaram diminui????o no n??mero de c??lulas pr??-inflamat??rias, IL-6 e TNF-?? (p<0,05), e aumento no n??mero da IL-10 (p<0,05) anti-inflamat??ria mesmo quando comparado como grupo n??o OVX (p<0,05). Assim conclui-se que a LED de baixa intensidade, nos par??metros estudados tem efeito reparador em processo de tendinopatia (tendinite) traum??tica em ratas (OVX).Submitted by Nadir Basilio (nadirsb@uninove.br) on 2015-07-21T15:29:23Z No. of bitstreams: 1 Carla Helrigle Silva.pdf: 884644 bytes, checksum: 450ed90ece95b161e2e9976cead3aea4 (MD5)Made available in DSpace on 2015-07-21T15:29:23Z (GMT). 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dc.title.por.fl_str_mv Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
title Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
spellingShingle Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
Silva, Carla Helrigle
inflama????o
ovariectomia
tendinite
citocinas
LED
inflammation
ovariectomy
tendonitis
cytokines
LED
CIENCIAS DA SAUDE
title_short Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
title_full Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
title_fullStr Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
title_full_unstemmed Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
title_sort Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios
author Silva, Carla Helrigle
author_facet Silva, Carla Helrigle
author_role author
dc.contributor.advisor1.fl_str_mv Carvalho, Regiane Albertini de
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2515657728339113
dc.contributor.referee1.fl_str_mv Costa, Maricilia Silva
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/7963390052508784
dc.contributor.referee2.fl_str_mv Leal Junior, Ernesto Cesar Pinto
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8166626486201116
dc.contributor.authorID.fl_str_mv 00003526160
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3362144866086155
dc.contributor.author.fl_str_mv Silva, Carla Helrigle
contributor_str_mv Carvalho, Regiane Albertini de
Costa, Maricilia Silva
Leal Junior, Ernesto Cesar Pinto
dc.subject.por.fl_str_mv inflama????o
ovariectomia
tendinite
citocinas
LED
topic inflama????o
ovariectomia
tendinite
citocinas
LED
inflammation
ovariectomy
tendonitis
cytokines
LED
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv inflammation
ovariectomy
tendonitis
cytokines
LED
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The aim of this experimental study was to investigate the effects of LED phototherapy on the inflammation process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of inflammatory mediators IL-6, IL-10 and TNF-??. Thirty-five female Wistar rats were divided into 4 groups: 3 ovariectomized groups, untreated (NTTA); treated (TTA) and control subdivided into experimental periods of 3 , 7 and 14 days, totaling 30 rats and one group (n = 5) not ovariectomized (not OVX). Tendinitis was induced by trauma using a 208g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 hours after tendonitis with LED therapy ( 945nm , power 32mW, energy of 3.84 J, power density of 0.06 W / cm ??, energy density of 7.68 J / cm ??, beam output area of 0.5 cm ?? and time of 120 s) and every 48 hours at 3, 7 and 14 days. Then, euthanasia was performed in a CO2 chamber 24h after the last treatment. The tendons were dissected and stored in liquid nitrogen at -196??C, thawed only at the time of immunoenzymatic test (ELISA). After analysis of results, groups treated with LED showed decrease in the number of pro-inflammatory cells, IL- 6 and TNF- ?? (p < 0.05) and increased number of anti-inflammatory IL -10 ( p <0.05) even when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED in the studied parameters has repairing effect on the tendinopathy process (tendonitis) caused by trauma in female rats (OVX).
publishDate 2013
dc.date.issued.fl_str_mv 2013-09-09
dc.date.accessioned.fl_str_mv 2015-07-21T15:29:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Silva, Carla Helrigle. Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios. 2013. 54 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo .
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/1141
identifier_str_mv Silva, Carla Helrigle. Efeitos da terapia led de baixa pot??ncia em ratas ovariectomizadas com trauma tend??neo: aspectos inflamat??rios. 2013. 54 f. Disserta????o( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo .
url http://bibliotecatede.uninove.br/handle/tede/1141
dc.language.iso.fl_str_mv por
language por
dc.relation.cnpq.fl_str_mv 8765449414823306929
dc.relation.confidence.fl_str_mv 600
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nove de Julho
dc.publisher.program.fl_str_mv Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de
dc.publisher.initials.fl_str_mv UNINOVE
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da Uninove
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