Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos

Detalhes bibliográficos
Autor(a) principal: Santana, Eduardo Tadeu
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
Texto Completo: http://bibliotecatede.uninove.br/handle/tede/1824
Resumo: The ligation of the anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we evaluated modifications of gene expression that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNA expressions of 9 different signal transduction pathways (signs of cell survival and metabolism, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis and inflammation) in rat left ventricle 1 week after MI promoted by either coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed after either MI procedures. Interestingly, mRNA expression levels and miRNA expressions were similar in both models after MI, with few specificities in each model. This study reports for the first time the global changes in rat cardiac mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses would enhance our present knowledge regarding altered pathophysiology of these two different MI models.
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spelling Silva Junior, Jose AntonioSilva Junior, Jose AntonioCarvalho, Paulo Tarso Camilo deSerra, Andrey JorgeTucci, Paulo Jose FerreiraGirardi, Adriana Castello Costahttp://lattes.cnpq.br/2331665675944960Santana, Eduardo Tadeu2018-06-21T18:29:06Z2015-12-18Santana, Eduardo Tadeu. Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos. 2015. 162 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1824The ligation of the anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we evaluated modifications of gene expression that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNA expressions of 9 different signal transduction pathways (signs of cell survival and metabolism, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis and inflammation) in rat left ventricle 1 week after MI promoted by either coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed after either MI procedures. Interestingly, mRNA expression levels and miRNA expressions were similar in both models after MI, with few specificities in each model. This study reports for the first time the global changes in rat cardiac mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses would enhance our present knowledge regarding altered pathophysiology of these two different MI models.A ligadura da art??ria coronariana descendente anterior ?? o modelo experimental mais comumente usado para induzir o infarto do mioc??rdio (IM) em roedores. Entretanto, uma elevada taxa de mortalidade na fase aguda e a heterogeneidade do tamanho do IM obtidos s??o desvantagens reconhecidas neste modelo. Em uma tentativa de resolver o problema, o nosso grupo desenvolveu recentemente um novo modelo experimental de insufici??ncia card??aca que se baseia na aplica????o de correntes de radiofrequ??ncia abla????o do mioc??rdio (AB-RF), produzindo IM com tamanhos homog??neos e com significativamente redu????o da mortalidade aguda. Al??m disso, altera????es estruturais e funcionais do cora????o deste modelo foram semelhantes aos observados em animais com infarto induzido por liga????o da art??ria coron??ria. Aqui, n??s avaliamos modifica????es da express??o de RNA mensageiro (RNAm) de genes ap??s IM induzido por oclus??o e abla????o. Foram analisadas as express??es de 48 RNAm de 9 diferentes vias de transdu????o de sinal (sinais de sobreviv??ncia celular e metabolismo, matriz extracelular, ciclo celular, estresse oxidativo, apoptose, sinaliza????o de c??lcio, marcadores de hipertrofia, angiog??nese e inflama????o) no ventr??culo esquerdo de ratos uma semana ap??s o IM promovido por oclus??o coron??ria e AB-RF. Al??m disso, a an??lise de alto rendimento de miRNA tamb??m foi avaliada ap??s ambos procedimentos de IM. Curiosamente, os n??veis de express??o de RNAm e express??o de miRNA foram semelhantes em ambos os modelos ap??s o IM, com algumas especificidades em cada modelo. Este estudo relata pela primeira vez as mudan??as globais nos conte??dos de RNAm e miRNA ap??s dois procedimentos de IM diferentes e identifica reguladores que podem modular a fisiopatologia desses dois modelos, culminando em insufici??ncia card??aca.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-21T18:29:06Z No. of bitstreams: 1 Eduardo Tadeu Santana.pdf: 3781492 bytes, checksum: 5fb6eff774c2c39d42bab565b9e807ef (MD5)Made available in DSpace on 2018-06-21T18:29:06Z (GMT). No. of bitstreams: 1 Eduardo Tadeu Santana.pdf: 3781492 bytes, checksum: 5fb6eff774c2c39d42bab565b9e807ef (MD5) Previous issue date: 2015-12-18application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Ci??ncias da Reabilita????oUNINOVEBrasilSa??deinfarto do mioc??rdioremodelamentotranscriptomaMicroRNAmyocardial infarctremodelingtranscriptomeMicroRNACIENCIAS DA SAUDECaracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratosGene expression characterization of signal transduction pathways in the remote myocardium after infarction induced by left ventricular ablation and coronary artery occlusion in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALEduardo Tadeu Santana.pdfEduardo Tadeu Santana.pdfapplication/pdf3781492http://localhost:8080/tede/bitstream/tede/1824/2/Eduardo+Tadeu+Santana.pdf5fb6eff774c2c39d42bab565b9e807efMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1824/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/18242019-10-17 18:43:11.702oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2019-10-17T21:43:11Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false
dc.title.por.fl_str_mv Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
dc.title.alternative.eng.fl_str_mv Gene expression characterization of signal transduction pathways in the remote myocardium after infarction induced by left ventricular ablation and coronary artery occlusion in rats
title Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
spellingShingle Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
Santana, Eduardo Tadeu
infarto do mioc??rdio
remodelamento
transcriptoma
MicroRNA
myocardial infarct
remodeling
transcriptome
MicroRNA
CIENCIAS DA SAUDE
title_short Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
title_full Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
title_fullStr Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
title_full_unstemmed Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
title_sort Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos
author Santana, Eduardo Tadeu
author_facet Santana, Eduardo Tadeu
author_role author
dc.contributor.advisor1.fl_str_mv Silva Junior, Jose Antonio
dc.contributor.referee1.fl_str_mv Silva Junior, Jose Antonio
dc.contributor.referee2.fl_str_mv Carvalho, Paulo Tarso Camilo de
dc.contributor.referee3.fl_str_mv Serra, Andrey Jorge
dc.contributor.referee4.fl_str_mv Tucci, Paulo Jose Ferreira
dc.contributor.referee5.fl_str_mv Girardi, Adriana Castello Costa
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2331665675944960
dc.contributor.author.fl_str_mv Santana, Eduardo Tadeu
contributor_str_mv Silva Junior, Jose Antonio
Silva Junior, Jose Antonio
Carvalho, Paulo Tarso Camilo de
Serra, Andrey Jorge
Tucci, Paulo Jose Ferreira
Girardi, Adriana Castello Costa
dc.subject.por.fl_str_mv infarto do mioc??rdio
remodelamento
transcriptoma
MicroRNA
topic infarto do mioc??rdio
remodelamento
transcriptoma
MicroRNA
myocardial infarct
remodeling
transcriptome
MicroRNA
CIENCIAS DA SAUDE
dc.subject.eng.fl_str_mv myocardial infarct
remodeling
transcriptome
MicroRNA
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE
description The ligation of the anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we evaluated modifications of gene expression that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNA expressions of 9 different signal transduction pathways (signs of cell survival and metabolism, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis and inflammation) in rat left ventricle 1 week after MI promoted by either coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed after either MI procedures. Interestingly, mRNA expression levels and miRNA expressions were similar in both models after MI, with few specificities in each model. This study reports for the first time the global changes in rat cardiac mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses would enhance our present knowledge regarding altered pathophysiology of these two different MI models.
publishDate 2015
dc.date.issued.fl_str_mv 2015-12-18
dc.date.accessioned.fl_str_mv 2018-06-21T18:29:06Z
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dc.identifier.citation.fl_str_mv Santana, Eduardo Tadeu. Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos. 2015. 162 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
dc.identifier.uri.fl_str_mv http://bibliotecatede.uninove.br/handle/tede/1824
identifier_str_mv Santana, Eduardo Tadeu. Caracteriza????o da express??o g??nica de vias de transdu????o do sinal no mioc??rdio remoto ao infarto induzido por abla????o ventricular esquerda e oclus??o da art??ria coron??ria em ratos. 2015. 162 f. Tese( Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o) - Universidade Nove de Julho, S??o Paulo.
url http://bibliotecatede.uninove.br/handle/tede/1824
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dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Sa??de
publisher.none.fl_str_mv Universidade Nove de Julho
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