Efeito da LED terapia em modelo experimental de fibrose pulmonar
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/2859 |
Resumo: | Pulmonary fibrosis (PF) is a chronic and progressive lung disease characterized by pulmonary parenchyma progressive lesion, inflammatory infiltration, and interstitial fibrosis. It is developed by excessive collagen deposition and other cellular matrix components, resulting in severe changes in the alveolar architecture. Considering the absence of effective treatment, the aim of this study was to investigate the effect of LED treatment on the development of PF. For this purpose, we used C57BL/6 mice submitted to induction of PF by bleomycin administration (1.5 U / kg) by orotracheal route and, after 14 days of the induction, mice were treated with LED once daily for 8 days. It was evaluated inflammatory and fibrotic parameters with or without LED treatment. Our results showed that treatment with LED significantly reduced the number of inflammatory cells in the alveolar space, collagen production, interstitial thickening, and static and dynamic pulmonary elastance. In addition, we observed reduced levels of IL-6 e CXCL1/KC released by pneumocytes type II in culture as well as reduced level of CXCL11/KC released by fibroblasts in culture. It can be concluded that the LED therapy improved both inflammatory and fibrotic parameters and, it can be a promisor therapy with low cost and absent of side effects. |
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Franco, Adriana Lino dos SantosFranco, Adriana Lino dos SantosOli, Ana Paula Ligeiro deLandgraf, Richardt Gamahttp://lattes.cnpq.br/8384687155975671Brochetti, Robson Alexandre2022-04-11T18:57:40Z2016-12-08Brochetti, Robson Alexandre. Efeito da LED terapia em modelo experimental de fibrose pulmonar. 2016. 51 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2859Pulmonary fibrosis (PF) is a chronic and progressive lung disease characterized by pulmonary parenchyma progressive lesion, inflammatory infiltration, and interstitial fibrosis. It is developed by excessive collagen deposition and other cellular matrix components, resulting in severe changes in the alveolar architecture. Considering the absence of effective treatment, the aim of this study was to investigate the effect of LED treatment on the development of PF. For this purpose, we used C57BL/6 mice submitted to induction of PF by bleomycin administration (1.5 U / kg) by orotracheal route and, after 14 days of the induction, mice were treated with LED once daily for 8 days. It was evaluated inflammatory and fibrotic parameters with or without LED treatment. Our results showed that treatment with LED significantly reduced the number of inflammatory cells in the alveolar space, collagen production, interstitial thickening, and static and dynamic pulmonary elastance. In addition, we observed reduced levels of IL-6 e CXCL1/KC released by pneumocytes type II in culture as well as reduced level of CXCL11/KC released by fibroblasts in culture. It can be concluded that the LED therapy improved both inflammatory and fibrotic parameters and, it can be a promisor therapy with low cost and absent of side effects.A fibrose pulmonar (FP) é uma doença pulmonar crônica e progressiva caracterizada por lesão progressiva do parênquima pulmonar, infiltrado inflamatório e fibrose no interstício. É desencadeada pela excessiva e desordenada deposição de colágeno e outros componentes de matriz extracelular, resultando em severas mudanças na arquitetura da parede do alvéolo. Em função da ausência de um tratamento eficaz, o objetivo do estudo foi investigar o efeito da LED terapia sobre o desenvolvimento da FP. Para tanto, foram utilizados camundongos C57BL/6 submetidos à indução de FP pela administração de bleomicina (1,5 U/Kg) por via orotraqueal e após 14 dias de indução, os camundongos foram tratados com LED 1x/dia durante 8 dias. Foram avaliados parâmetros inflamatórios e fibróticos na presença ou não de terapia com LED. Os nossos resultados mostraram que o tratamento com LED reduziu o número de células inflamatórias no espaço alveolar, a produção de colágeno, o espessamento intersticial e a elastância pulmonar dinâmica e estática. Ainda, notamos redução na liberação de IL-6 e CXCL1/KC pelos pneumócitos tipo II em cultura e redução de CXCL11/KC pelos fibroblastos em cultura. Pode-se concluir que a terapia com LED melhorou importantes parâmetros tanto inflamatórios quanto fibróticos e pode ser uma promissora terapia a baixo custo e ausente de efeitos colaterais.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-11T18:57:40Z No. of bitstreams: 1 Robson Alexandre Brochetti.pdf: 1590602 bytes, checksum: 4baeb19772cd347c9df48cb8e58060a5 (MD5)Made available in DSpace on 2022-04-11T18:57:40Z (GMT). No. of bitstreams: 1 Robson Alexandre Brochetti.pdf: 1590602 bytes, checksum: 4baeb19772cd347c9df48cb8e58060a5 (MD5) Previous issue date: 2016-12-08application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdefibrose pulmonarinflamação pulmonarremodelamento das vias aéreasfunção pulmonarLED terapiapulmonary fibrosislung inflammationairways remodelinglung functionLED therapyCIENCIAS DA SAUDEEfeito da LED terapia em modelo experimental de fibrose pulmonarEffect of LED therapy on an experimental model of pulmonary fibrosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALRobson Alexandre Brochetti.pdfRobson Alexandre Brochetti.pdfapplication/pdf1590602http://localhost:8080/tede/bitstream/tede/2859/2/Robson+Alexandre+Brochetti.pdf4baeb19772cd347c9df48cb8e58060a5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2859/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/28592022-04-11 15:57:40.983oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-04-11T18:57:40Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
dc.title.alternative.eng.fl_str_mv |
Effect of LED therapy on an experimental model of pulmonary fibrosis |
title |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
spellingShingle |
Efeito da LED terapia em modelo experimental de fibrose pulmonar Brochetti, Robson Alexandre fibrose pulmonar inflamação pulmonar remodelamento das vias aéreas função pulmonar LED terapia pulmonary fibrosis lung inflammation airways remodeling lung function LED therapy CIENCIAS DA SAUDE |
title_short |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
title_full |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
title_fullStr |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
title_full_unstemmed |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
title_sort |
Efeito da LED terapia em modelo experimental de fibrose pulmonar |
author |
Brochetti, Robson Alexandre |
author_facet |
Brochetti, Robson Alexandre |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.referee1.fl_str_mv |
Franco, Adriana Lino dos Santos |
dc.contributor.referee2.fl_str_mv |
Oli, Ana Paula Ligeiro de |
dc.contributor.referee3.fl_str_mv |
Landgraf, Richardt Gama |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8384687155975671 |
dc.contributor.author.fl_str_mv |
Brochetti, Robson Alexandre |
contributor_str_mv |
Franco, Adriana Lino dos Santos Franco, Adriana Lino dos Santos Oli, Ana Paula Ligeiro de Landgraf, Richardt Gama |
dc.subject.por.fl_str_mv |
fibrose pulmonar inflamação pulmonar remodelamento das vias aéreas função pulmonar LED terapia |
topic |
fibrose pulmonar inflamação pulmonar remodelamento das vias aéreas função pulmonar LED terapia pulmonary fibrosis lung inflammation airways remodeling lung function LED therapy CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
pulmonary fibrosis lung inflammation airways remodeling lung function LED therapy |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Pulmonary fibrosis (PF) is a chronic and progressive lung disease characterized by pulmonary parenchyma progressive lesion, inflammatory infiltration, and interstitial fibrosis. It is developed by excessive collagen deposition and other cellular matrix components, resulting in severe changes in the alveolar architecture. Considering the absence of effective treatment, the aim of this study was to investigate the effect of LED treatment on the development of PF. For this purpose, we used C57BL/6 mice submitted to induction of PF by bleomycin administration (1.5 U / kg) by orotracheal route and, after 14 days of the induction, mice were treated with LED once daily for 8 days. It was evaluated inflammatory and fibrotic parameters with or without LED treatment. Our results showed that treatment with LED significantly reduced the number of inflammatory cells in the alveolar space, collagen production, interstitial thickening, and static and dynamic pulmonary elastance. In addition, we observed reduced levels of IL-6 e CXCL1/KC released by pneumocytes type II in culture as well as reduced level of CXCL11/KC released by fibroblasts in culture. It can be concluded that the LED therapy improved both inflammatory and fibrotic parameters and, it can be a promisor therapy with low cost and absent of side effects. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-08 |
dc.date.accessioned.fl_str_mv |
2022-04-11T18:57:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Brochetti, Robson Alexandre. Efeito da LED terapia em modelo experimental de fibrose pulmonar. 2016. 51 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2859 |
identifier_str_mv |
Brochetti, Robson Alexandre. Efeito da LED terapia em modelo experimental de fibrose pulmonar. 2016. 51 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/2859 |
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por |
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