An??lise da viabilidade de utiliza????o da saliva como fonte de material biol??gico para o estudo de biomarcadores na Doen??a Pulmonar Obstrutiva Cr??nica (DPOC)

• Autor(a) principal: Santos, Alecsandra Aparecida dos
• Data de Publicação: 2008
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da Uninove
• Texto Completo: http://bibliotecatede.uninove.br/tede/handle/tede/806
• topic: Doen??a pulmonar obstrutiva cr??nica; Metaloproteinase 2 da matriz; Metaloproteinase 9 da matriz; Chronic obstructive pulmonary disease; matrix metalloproteinase 9; matrix metalloproteinase 2; CIENCIAS DA SAUDE

The increased expression of matrix metalloproteinases (MMPs) is considered to be a key factor in the development of COPD. We performed this study to quantify the expression of MMP-2 and MMP-9 in saliva of COPD patients and healthy controls, and to evaluate feasibility of saliva compounds as a biological material source for the study of biomarkers in COPD. Methods: COPD patients (n=16) and healthy controls (n=9) were selected. In the both group spirometry test was performed and saliva samples were obtained from each subject. The saliva levels of MMP-2 and MMP-9 were determined by Western Blot. Results: MMP-2 and MMP-9 were significantly higher in COPD patients than in control subjects. MMP-2 concentration was correlated negatively with forced expiatory volume in one second (FEV1) (r= -0,582; p= 0,014) in COPD patients. Conclusion: The current findings open new perspectives to study specific biomarkers in saliva to predict and monitor airway obstruction in COPD patients