Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/1779 |
Resumo: | The aging changes the activation and proliferation of satellite cells and inflammatory cells in skeletal muscle and fibroblasts promotes increased, decreased vascularization and thickening of the basal lamina. The aim of this study was to evaluate the effects of LBP on the tibialis anterior (TA) muscle of aged rats after cryoinjury on morphological, on the expression of IL-6 and IL-1 beta, distribution and collagen organization. It was performed histological photomicrographs of the TA muscle stained with hematoxylin-eosin, Picrosirius Red, and immunohistochemistry for IL-6 and IL-1beta. The results were quantitatively and qualitatively in Image J software. The study was approved by CEUA-UNINOVE and were used n= 30 legs of male rats Wistar divided into 03 experimental groups: - Group 1 Control (n= 4): animals uninjured or LBP-treated; Group 2 cryoinjury LBP untreated (n= 13) and Group 3 treated with LBP infrared cryoinjury (n= 13). The LBP was applied to muscle after 2h of induction of injury and consisted of daily application until the day of sacrifice (Groups 1, 3 or 7 days) according to the study group. We were using the parameters (?? = 780nm, 1000mW / cm2 power density, power 40mW; 3,2J total energy, time of 10s). In histomorphological analysis, seven days the treated group had a significant decrease in infiltration of inflammatory cells and increased immature fibers and new blood vessels compared to Group 7 days did not-treated (p <0.05). In the analysis of distribution and collagen organization, there was a control group difference from the group treated 7 days and non-treated (p <0.05). The expression of IL-6 by immunohistochemistry, was higher in all study groups (p <0.05) compared to control, but there was no difference between the groups treated injured or not. The IL-1beta expression, there was increased expression in 3 days treated compared to group 1 and 7 days treated. In conclusion, the LPB shown modulatory effect on muscle repair process in aged animals. |
id |
NOVE_a172d2eb0800d45c2c7adb8123f0239a |
---|---|
oai_identifier_str |
oai:localhost:tede/1779 |
network_acronym_str |
NOVE |
network_name_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
repository_id_str |
|
spelling |
Ferrari, Raquel Agnelli MesquitaFerrari, Raquel Agnelli MesquitaFernandes, Kristianne Porta SantosHorliana, Anna Carolina Ratto TempestiniNavarro, Ricardo ScarparoNunes, Fabio Daumashttp://lattes.cnpq.br/9059872659678287Brito, Adriana de2018-06-15T13:56:37Z2016-03-02Brito, Adriana de. Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso. 2016. 57 f. Tese( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo.http://bibliotecatede.uninove.br/handle/tede/1779The aging changes the activation and proliferation of satellite cells and inflammatory cells in skeletal muscle and fibroblasts promotes increased, decreased vascularization and thickening of the basal lamina. The aim of this study was to evaluate the effects of LBP on the tibialis anterior (TA) muscle of aged rats after cryoinjury on morphological, on the expression of IL-6 and IL-1 beta, distribution and collagen organization. It was performed histological photomicrographs of the TA muscle stained with hematoxylin-eosin, Picrosirius Red, and immunohistochemistry for IL-6 and IL-1beta. The results were quantitatively and qualitatively in Image J software. The study was approved by CEUA-UNINOVE and were used n= 30 legs of male rats Wistar divided into 03 experimental groups: - Group 1 Control (n= 4): animals uninjured or LBP-treated; Group 2 cryoinjury LBP untreated (n= 13) and Group 3 treated with LBP infrared cryoinjury (n= 13). The LBP was applied to muscle after 2h of induction of injury and consisted of daily application until the day of sacrifice (Groups 1, 3 or 7 days) according to the study group. We were using the parameters (?? = 780nm, 1000mW / cm2 power density, power 40mW; 3,2J total energy, time of 10s). In histomorphological analysis, seven days the treated group had a significant decrease in infiltration of inflammatory cells and increased immature fibers and new blood vessels compared to Group 7 days did not-treated (p <0.05). In the analysis of distribution and collagen organization, there was a control group difference from the group treated 7 days and non-treated (p <0.05). The expression of IL-6 by immunohistochemistry, was higher in all study groups (p <0.05) compared to control, but there was no difference between the groups treated injured or not. The IL-1beta expression, there was increased expression in 3 days treated compared to group 1 and 7 days treated. In conclusion, the LPB shown modulatory effect on muscle repair process in aged animals.O envelhecimento altera a ativa????o e prolifera????o de c??lulas sat??lites e c??lulas inflamat??rias no m??sculo esquel??tico e promove o aumento de fibroblastos, diminui????o da vasculariza????o e espessamento da l??mina basal. O objetivo do presente estudo foi avaliar os efeitos do LBP no m??sculo tibial anterior (TA) de ratos idosos ap??s crioles??o sobre os aspectos morfol??gicos, sobre a express??o de Il-6 e IL-1 beta, a distribui????o e organiza????o do col??geno. Foi realizada fotomicrografia histol??gica do m??sculo TA corados com HE, Picrosirius Red e Imunohistoqu??mica para IL-6 e IL-1 beta. Os resultados foram quantitativa e qualitativamente no software Image J. O estudo foi aprovado pelo CEUA-UNINOVE e foram utilizadas n=30 patas de ratos machos Wistar distribu??das em 03 grupos experimentais: - Grupo 1 Controle (n=4): animais n??o lesionados ou tratados com LBP; Grupo 2 Crioles??o sem tratamento LBP (n= 13) e Grupo 3 Crioles??o tratado com LBP infravermelho (n=13). O LBP foi aplicado no TA ap??s 2h da indu????o da les??o e consistiu de aplica????es di??rias at?? o dia do sacrif??cio (grupos 1, 3 ou 7 dias) de acordo com o grupo estudado. Foram utilizando os par??metros (??= 780nm; 1000mW/cm2 densidade de pot??ncia; 40mW de pot??ncia; 3,2J de energia total; tempo de 10s). Na an??lise histomorfol??gica, o grupo 7 dias tratado teve diminui????o significantes de infiltrado de c??lulas inflamat??rias e aumento de fibras imaturas e novos vasos sangu??neos, comparado ao grupo 7 dias n??o-tratado (p<0,05). Na an??lise da distribui????o e organiza????o do col??geno, houve diferen??a do grupo controle em rela????o ao grupo 7 dias tratado e n??o-tratado (p<0,05). A express??o de IL-6 por imunohistoqu??mica, foi maior em todos os grupos estudos (p<0,05) comparado ao controle, por??m n??o houve diferen??a entre os grupos lesionados tratados ou n??o. Quanto a express??o de IL-1 beta, houve aumento da express??o em 3 dias tratados comparados ao grupo 1 e 7 dias tratados. Em conclus??o, o LPB demonstrou efeito modulat??rio no processo de reparo muscular em animais idosos.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2018-06-15T13:56:37Z No. of bitstreams: 1 Adriana de Brito.pdf: 2322726 bytes, checksum: 952b5de2e151ee3aa44078fcedc7300a (MD5)Made available in DSpace on 2018-06-15T13:56:37Z (GMT). No. of bitstreams: 1 Adriana de Brito.pdf: 2322726 bytes, checksum: 952b5de2e151ee3aa44078fcedc7300a (MD5) Previous issue date: 2016-03-02application/pdfporUniversidade Nove de JulhoPrograma de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??deUNINOVEBrasilSa??deenvelhecimentoregenera????olaser de baixa pot??nciaLBPcol??genom??sculo esquel??ticoagingregenerationlow-level laser therapyLLLTcollagenskeletal muscleCIENCIAS DA SAUDEEfeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idosoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALAdriana de Brito.pdfAdriana de Brito.pdfapplication/pdf2322726http://localhost:8080/tede/bitstream/tede/1779/2/Adriana+de+Brito.pdf952b5de2e151ee3aa44078fcedc7300aMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/1779/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/17792018-06-15 10:56:37.607oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2018-06-15T13:56:37Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
title |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
spellingShingle |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso Brito, Adriana de envelhecimento regenera????o laser de baixa pot??ncia LBP col??geno m??sculo esquel??tico aging regeneration low-level laser therapy LLLT collagen skeletal muscle CIENCIAS DA SAUDE |
title_short |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
title_full |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
title_fullStr |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
title_full_unstemmed |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
title_sort |
Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso |
author |
Brito, Adriana de |
author_facet |
Brito, Adriana de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee1.fl_str_mv |
Ferrari, Raquel Agnelli Mesquita |
dc.contributor.referee2.fl_str_mv |
Fernandes, Kristianne Porta Santos |
dc.contributor.referee3.fl_str_mv |
Horliana, Anna Carolina Ratto Tempestini |
dc.contributor.referee4.fl_str_mv |
Navarro, Ricardo Scarparo |
dc.contributor.referee5.fl_str_mv |
Nunes, Fabio Daumas |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9059872659678287 |
dc.contributor.author.fl_str_mv |
Brito, Adriana de |
contributor_str_mv |
Ferrari, Raquel Agnelli Mesquita Ferrari, Raquel Agnelli Mesquita Fernandes, Kristianne Porta Santos Horliana, Anna Carolina Ratto Tempestini Navarro, Ricardo Scarparo Nunes, Fabio Daumas |
dc.subject.por.fl_str_mv |
envelhecimento regenera????o laser de baixa pot??ncia LBP col??geno m??sculo esquel??tico |
topic |
envelhecimento regenera????o laser de baixa pot??ncia LBP col??geno m??sculo esquel??tico aging regeneration low-level laser therapy LLLT collagen skeletal muscle CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
aging regeneration low-level laser therapy LLLT collagen skeletal muscle |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
The aging changes the activation and proliferation of satellite cells and inflammatory cells in skeletal muscle and fibroblasts promotes increased, decreased vascularization and thickening of the basal lamina. The aim of this study was to evaluate the effects of LBP on the tibialis anterior (TA) muscle of aged rats after cryoinjury on morphological, on the expression of IL-6 and IL-1 beta, distribution and collagen organization. It was performed histological photomicrographs of the TA muscle stained with hematoxylin-eosin, Picrosirius Red, and immunohistochemistry for IL-6 and IL-1beta. The results were quantitatively and qualitatively in Image J software. The study was approved by CEUA-UNINOVE and were used n= 30 legs of male rats Wistar divided into 03 experimental groups: - Group 1 Control (n= 4): animals uninjured or LBP-treated; Group 2 cryoinjury LBP untreated (n= 13) and Group 3 treated with LBP infrared cryoinjury (n= 13). The LBP was applied to muscle after 2h of induction of injury and consisted of daily application until the day of sacrifice (Groups 1, 3 or 7 days) according to the study group. We were using the parameters (?? = 780nm, 1000mW / cm2 power density, power 40mW; 3,2J total energy, time of 10s). In histomorphological analysis, seven days the treated group had a significant decrease in infiltration of inflammatory cells and increased immature fibers and new blood vessels compared to Group 7 days did not-treated (p <0.05). In the analysis of distribution and collagen organization, there was a control group difference from the group treated 7 days and non-treated (p <0.05). The expression of IL-6 by immunohistochemistry, was higher in all study groups (p <0.05) compared to control, but there was no difference between the groups treated injured or not. The IL-1beta expression, there was increased expression in 3 days treated compared to group 1 and 7 days treated. In conclusion, the LPB shown modulatory effect on muscle repair process in aged animals. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-03-02 |
dc.date.accessioned.fl_str_mv |
2018-06-15T13:56:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brito, Adriana de. Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso. 2016. 57 f. Tese( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/1779 |
identifier_str_mv |
Brito, Adriana de. Efeito da terapia laser de baixa pot??ncia sobre o remodelamento do tecido conjuntivo e a regenera????o do m??sculo esquel??tico de ratos idoso. 2016. 57 f. Tese( Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de) - Universidade Nove de Julho, S??o Paulo. |
url |
http://bibliotecatede.uninove.br/handle/tede/1779 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
8765449414823306929 |
dc.relation.confidence.fl_str_mv |
600 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.publisher.program.fl_str_mv |
Programa de P??s-Gradua????o em Biofot??nica Aplicada ??s Ci??ncias da Sa??de |
dc.publisher.initials.fl_str_mv |
UNINOVE |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Sa??de |
publisher.none.fl_str_mv |
Universidade Nove de Julho |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da Uninove instname:Universidade Nove de Julho (UNINOVE) instacron:UNINOVE |
instname_str |
Universidade Nove de Julho (UNINOVE) |
instacron_str |
UNINOVE |
institution |
UNINOVE |
reponame_str |
Biblioteca Digital de Teses e Dissertações da Uninove |
collection |
Biblioteca Digital de Teses e Dissertações da Uninove |
bitstream.url.fl_str_mv |
http://localhost:8080/tede/bitstream/tede/1779/2/Adriana+de+Brito.pdf http://localhost:8080/tede/bitstream/tede/1779/1/license.txt |
bitstream.checksum.fl_str_mv |
952b5de2e151ee3aa44078fcedc7300a bd3efa91386c1718a7f26a329fdcb468 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE) |
repository.mail.fl_str_mv |
bibliotecatede@uninove.br||bibliotecatede@uninove.br |
_version_ |
1811016874196992000 |