Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da Uninove |
Texto Completo: | http://bibliotecatede.uninove.br/handle/tede/2882 |
Resumo: | Periodontal disease (PD) is a highly prevalent chronic inflammatory disease around the world, affecting approximately 10% of the adult population, caused by bacterial biofilm. Antimicrobial therapy with blue light (aBLT) is based on the interaction of light with endogenous photosensitizers produced by microorganisms, such as metal-free porphyrins and flavins, in the generation of reactive oxygen species (ROS) and cell elimination. The goal of this work is to evaluate the potential of bacterial death by aBLT and the influence of BHI (brain heart infusion) culture media and blood agar on the death curve of the periodontopathic bacteria Aggregatibacter actninomycetencomitans. For this work, we u LED emitting at 403nm ± 15 with 1W of radiant power, the irradiance of 588,2 mW / cm2, and irradiation duration of 0, 1, 5, 10, 30 and 60 minutes in 2 different groups: A. actinomycetencomitans cultivated in BHI and A. actinomycetencomitans in blood agar. The plates were incubated in microaerophilia, in a bacteriological greenhouse, with a temperature regulated at 37◦ C during a period of 48h to count the colony-forming units (CFU / mL) and performed in triplicate. Spectroscopy and fluorescence microscopy were also carried out to investigate the presence of photosensitizers inside the microorganisms. Results: There was no statistical difference in the survival fraction of the colonies when A. actinomycetencomitans was cultivated in different culture media (p>0,05), however when the irradiation time reached 30 minutes (1.058 J/cm2), a biological and statistical reduction of microorganisms in both media was observed (p<0,05). The absorption spectrum and the analysis of fluorescence microscopy give indications of the presence of endogenous porphyrins inside these bacteria, regardless of the culture media. |
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Deana, Alessandro MeloPrates, Renato AraujoDeana, Alessandro MeloPavani, ChristianeNunez, Silvia Cristinahttp://lattes.cnpq.br/9952298610064070Salviatto, Luciana Toledo Costa2022-04-12T19:07:36Z2021-12-10Salviatto, Luciana Toledo Costa. Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul. 2021. 55 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo.http://bibliotecatede.uninove.br/handle/tede/2882Periodontal disease (PD) is a highly prevalent chronic inflammatory disease around the world, affecting approximately 10% of the adult population, caused by bacterial biofilm. Antimicrobial therapy with blue light (aBLT) is based on the interaction of light with endogenous photosensitizers produced by microorganisms, such as metal-free porphyrins and flavins, in the generation of reactive oxygen species (ROS) and cell elimination. The goal of this work is to evaluate the potential of bacterial death by aBLT and the influence of BHI (brain heart infusion) culture media and blood agar on the death curve of the periodontopathic bacteria Aggregatibacter actninomycetencomitans. For this work, we u LED emitting at 403nm ± 15 with 1W of radiant power, the irradiance of 588,2 mW / cm2, and irradiation duration of 0, 1, 5, 10, 30 and 60 minutes in 2 different groups: A. actinomycetencomitans cultivated in BHI and A. actinomycetencomitans in blood agar. The plates were incubated in microaerophilia, in a bacteriological greenhouse, with a temperature regulated at 37◦ C during a period of 48h to count the colony-forming units (CFU / mL) and performed in triplicate. Spectroscopy and fluorescence microscopy were also carried out to investigate the presence of photosensitizers inside the microorganisms. Results: There was no statistical difference in the survival fraction of the colonies when A. actinomycetencomitans was cultivated in different culture media (p>0,05), however when the irradiation time reached 30 minutes (1.058 J/cm2), a biological and statistical reduction of microorganisms in both media was observed (p<0,05). The absorption spectrum and the analysis of fluorescence microscopy give indications of the presence of endogenous porphyrins inside these bacteria, regardless of the culture media.A doença periodontal (DP) é uma doença crônica inflamatória altamente prevalente ao redor do mundo, podendo atingir cerca de 10% da população adulta, causada pelo biofilme bacteriano. A terapia antimicrobiana com luz azul (aBLT) baseia-se na interação da luz com fotossensibilizadores endógenos produzidos pelos micro-organismos como as porfirinas livres de metal e flavinas, na formação de espécies reativas de oxigênio (ROS) e morte celular. O objetivo do presente estudo é avaliar o potencial de morte bacteriana induzida pela aBLT e a influência dos meios de cultura BHI (brain heart infusion) e ágar sangue na curva de morte da bactéria periodonto patógena Aggregatibacter actninomycetencomitans. Para este trabalho, utilizou-se um LED emitindo em 403 nm ± 15 com a potência de 1W, irradiância de 588,2 mW/cm2, e tempos de irradiação de 0,1,5,10,30 e 60 minutos em 2 diferentes grupos: A. actinomycetencomitans cultivado em BHI e A. actinomycetencomitans em ágar sangue. As placas foram incubadas em microaerofilia, em estufa bacteriológica, com temperatura regulada em 37º C por um período de 48h para contagem das unidades formadoras de colônia (UFC/mL) e realizados em triplicata. Espectroscopia e microscopia de fluorescência também foram realizadas para investigar a presença de fotossensibilizadores no interior dos micro-organismos. Resultados: Não houve diferença estatística na fração de sobrevivência das colônias quando A. actinomycetencomitans foi cultivado em diferentes meios de cultura (p> 0,05), porém quando o tempo de irradiação atingiu 30 minutos (1,058 J / cm2), foi observada redução biológica e estatística dos microrganismos em ambos os meios (p <0,05). O espectro de absorção e a análise por microscopia de fluorescência sugerem a presença de porfirinas endógenas no interior dessas bactérias, independentemente do meio de cultura.Submitted by Nadir Basilio (nadirsb@uninove.br) on 2022-04-12T19:07:36Z No. of bitstreams: 1 Luciana Toledo Costa Salviatto.pdf: 1477114 bytes, checksum: 1ef0f9610f780f2e97a6c361759176d4 (MD5)Made available in DSpace on 2022-04-12T19:07:36Z (GMT). No. of bitstreams: 1 Luciana Toledo Costa Salviatto.pdf: 1477114 bytes, checksum: 1ef0f9610f780f2e97a6c361759176d4 (MD5) Previous issue date: 2021-12-10application/pdfporUniversidade Nove de JulhoPrograma de Pós-Graduação em Biofotônica Aplicada às Ciências da SaúdeUNINOVEBrasilSaúdefotossensibilizadores endógenosterapia antimicrobiana com luz azulaggregatibacter actinomycetencomitansperiodontiteporfirinasLEDendogenous photosensitizersantimicrobial blue lightaggregatibacter actnomycetencomitansperiodontitisporphyrinLEDCIENCIAS DA SAUDEEstudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azulStudy of the influence of the culture medium on the Aggregatibacter actinomycetencomitans dead curve induced by antimicrobial therapy with blue lightinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis8765449414823306929600info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da Uninoveinstname:Universidade Nove de Julho (UNINOVE)instacron:UNINOVEORIGINALLuciana Toledo Costa Salviatto.pdfLuciana Toledo Costa Salviatto.pdfapplication/pdf1477114http://localhost:8080/tede/bitstream/tede/2882/2/Luciana+Toledo+Costa+Salviatto.pdf1ef0f9610f780f2e97a6c361759176d4MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82165http://localhost:8080/tede/bitstream/tede/2882/1/license.txtbd3efa91386c1718a7f26a329fdcb468MD51tede/28822022-05-27 18:07:18.727oai:localhost: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Biblioteca Digital de Teses e Dissertaçõeshttp://bibliotecatede.uninove.br/PRIhttp://bibliotecatede.uninove.br/oai/requestbibliotecatede@uninove.br||bibliotecatede@uninove.bropendoar:2022-05-27T21:07:18Biblioteca Digital de Teses e Dissertações da Uninove - Universidade Nove de Julho (UNINOVE)false |
dc.title.por.fl_str_mv |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
dc.title.alternative.eng.fl_str_mv |
Study of the influence of the culture medium on the Aggregatibacter actinomycetencomitans dead curve induced by antimicrobial therapy with blue light |
title |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
spellingShingle |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul Salviatto, Luciana Toledo Costa fotossensibilizadores endógenos terapia antimicrobiana com luz azul aggregatibacter actinomycetencomitans periodontite porfirinas LED endogenous photosensitizers antimicrobial blue light aggregatibacter actnomycetencomitans periodontitis porphyrin LED CIENCIAS DA SAUDE |
title_short |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
title_full |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
title_fullStr |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
title_full_unstemmed |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
title_sort |
Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul |
author |
Salviatto, Luciana Toledo Costa |
author_facet |
Salviatto, Luciana Toledo Costa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Deana, Alessandro Melo |
dc.contributor.advisor-co1.fl_str_mv |
Prates, Renato Araujo |
dc.contributor.referee1.fl_str_mv |
Deana, Alessandro Melo |
dc.contributor.referee2.fl_str_mv |
Pavani, Christiane |
dc.contributor.referee3.fl_str_mv |
Nunez, Silvia Cristina |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9952298610064070 |
dc.contributor.author.fl_str_mv |
Salviatto, Luciana Toledo Costa |
contributor_str_mv |
Deana, Alessandro Melo Prates, Renato Araujo Deana, Alessandro Melo Pavani, Christiane Nunez, Silvia Cristina |
dc.subject.por.fl_str_mv |
fotossensibilizadores endógenos terapia antimicrobiana com luz azul aggregatibacter actinomycetencomitans periodontite porfirinas LED |
topic |
fotossensibilizadores endógenos terapia antimicrobiana com luz azul aggregatibacter actinomycetencomitans periodontite porfirinas LED endogenous photosensitizers antimicrobial blue light aggregatibacter actnomycetencomitans periodontitis porphyrin LED CIENCIAS DA SAUDE |
dc.subject.eng.fl_str_mv |
endogenous photosensitizers antimicrobial blue light aggregatibacter actnomycetencomitans periodontitis porphyrin LED |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE |
description |
Periodontal disease (PD) is a highly prevalent chronic inflammatory disease around the world, affecting approximately 10% of the adult population, caused by bacterial biofilm. Antimicrobial therapy with blue light (aBLT) is based on the interaction of light with endogenous photosensitizers produced by microorganisms, such as metal-free porphyrins and flavins, in the generation of reactive oxygen species (ROS) and cell elimination. The goal of this work is to evaluate the potential of bacterial death by aBLT and the influence of BHI (brain heart infusion) culture media and blood agar on the death curve of the periodontopathic bacteria Aggregatibacter actninomycetencomitans. For this work, we u LED emitting at 403nm ± 15 with 1W of radiant power, the irradiance of 588,2 mW / cm2, and irradiation duration of 0, 1, 5, 10, 30 and 60 minutes in 2 different groups: A. actinomycetencomitans cultivated in BHI and A. actinomycetencomitans in blood agar. The plates were incubated in microaerophilia, in a bacteriological greenhouse, with a temperature regulated at 37◦ C during a period of 48h to count the colony-forming units (CFU / mL) and performed in triplicate. Spectroscopy and fluorescence microscopy were also carried out to investigate the presence of photosensitizers inside the microorganisms. Results: There was no statistical difference in the survival fraction of the colonies when A. actinomycetencomitans was cultivated in different culture media (p>0,05), however when the irradiation time reached 30 minutes (1.058 J/cm2), a biological and statistical reduction of microorganisms in both media was observed (p<0,05). The absorption spectrum and the analysis of fluorescence microscopy give indications of the presence of endogenous porphyrins inside these bacteria, regardless of the culture media. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-12-10 |
dc.date.accessioned.fl_str_mv |
2022-04-12T19:07:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
Salviatto, Luciana Toledo Costa. Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul. 2021. 55 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
dc.identifier.uri.fl_str_mv |
http://bibliotecatede.uninove.br/handle/tede/2882 |
identifier_str_mv |
Salviatto, Luciana Toledo Costa. Estudo da influência do meio de cultura na curva de morte de Aggregatibacter actinomycetencomitans induzida pela terapia antimicrobiana com luz azul. 2021. 55 f. Dissertação( Programa de Pós-Graduação em Biofotônica Aplicada às Ciências da Saúde) - Universidade Nove de Julho, São Paulo. |
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http://bibliotecatede.uninove.br/handle/tede/2882 |
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