The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Psychology & Neuroscience (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-32882009000200016 |
Resumo: | In order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats - the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) - were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABA A receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABA A receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen. |
id |
PUCRJ-1_998670b473e7f74bcbccb8cd06e4575b |
---|---|
oai_identifier_str |
oai:scielo:S1983-32882009000200016 |
network_acronym_str |
PUCRJ-1 |
network_name_str |
Psychology & Neuroscience (Online) |
repository_id_str |
|
spelling |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of ratsdiazepamestrous cycleestrogenanxietyelevated T-mazeIn order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats - the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) - were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABA A receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABA A receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen.Pontificia Universidade Católica do Rio de JaneiroUniversidade de BrasíliaUniversidade de São Paulo2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-32882009000200016Psychology & Neuroscience v.2 n.2 2009reponame:Psychology & Neuroscience (Online)instname:Instituto Brasileiro de Neuropsicologia e Comportamento (IBNeC)instacron:PUCRJ10.3922/j.psns.2009.2.016info:eu-repo/semantics/openAccessGouveia Jr,AmauriAntunes,GabrielaMaximino,CaioMorato,Silvioeng2011-01-17T00:00:00Zoai:scielo:S1983-32882009000200016Revistahttps://www.apa.org/pubs/journals/pnePRIhttps://old.scielo.br/oai/scielo-oai.phppsycneuro@psycneuro.org1983-32881984-3054opendoar:2011-01-17T00:00Psychology & Neuroscience (Online) - Instituto Brasileiro de Neuropsicologia e Comportamento (IBNeC)false |
dc.title.none.fl_str_mv |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
spellingShingle |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats Gouveia Jr,Amauri diazepam estrous cycle estrogen anxiety elevated T-maze |
title_short |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_full |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_fullStr |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_full_unstemmed |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_sort |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
author |
Gouveia Jr,Amauri |
author_facet |
Gouveia Jr,Amauri Antunes,Gabriela Maximino,Caio Morato,Silvio |
author_role |
author |
author2 |
Antunes,Gabriela Maximino,Caio Morato,Silvio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Gouveia Jr,Amauri Antunes,Gabriela Maximino,Caio Morato,Silvio |
dc.subject.por.fl_str_mv |
diazepam estrous cycle estrogen anxiety elevated T-maze |
topic |
diazepam estrous cycle estrogen anxiety elevated T-maze |
description |
In order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats - the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) - were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABA A receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABA A receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-32882009000200016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-32882009000200016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3922/j.psns.2009.2.016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Pontificia Universidade Católica do Rio de Janeiro Universidade de Brasília Universidade de São Paulo |
publisher.none.fl_str_mv |
Pontificia Universidade Católica do Rio de Janeiro Universidade de Brasília Universidade de São Paulo |
dc.source.none.fl_str_mv |
Psychology & Neuroscience v.2 n.2 2009 reponame:Psychology & Neuroscience (Online) instname:Instituto Brasileiro de Neuropsicologia e Comportamento (IBNeC) instacron:PUCRJ |
instname_str |
Instituto Brasileiro de Neuropsicologia e Comportamento (IBNeC) |
instacron_str |
PUCRJ |
institution |
PUCRJ |
reponame_str |
Psychology & Neuroscience (Online) |
collection |
Psychology & Neuroscience (Online) |
repository.name.fl_str_mv |
Psychology & Neuroscience (Online) - Instituto Brasileiro de Neuropsicologia e Comportamento (IBNeC) |
repository.mail.fl_str_mv |
psycneuro@psycneuro.org |
_version_ |
1754821072322035712 |