Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods

Detalhes bibliográficos
Autor(a) principal: Fagundes de BARROS, Natália Eudes
Data de Publicação: 2023
Outros Autores: Morais OLIVEIRA, Edna Maria, MARIN, Victor Augustus
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Nutrição
Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9561
Resumo: Detection of genetically modified organisms in the food chain is an important issue for all subjects involved in raw material control, food industry and distribution. Both labeling and traceability of genetically modified organisms are current issues that are considered for trade and regulation. Currently, labeling of genetically modified foods containing detectable transgenic material is required by the Brazilian legislation. The Brazilian government published the Decree nº 4.680 in April 2003, which requires labeling for all foods or food ingredients, with a stricter labeling threshold of 1%. Although polymerase chain reaction technology hassome limitations, the high sensitivity and specificity explain why it has been the first choice of most analytical laboratories interested in detection of genetically modified organisms and their derived products. Among the currently available methods, polymerase chain reaction-based methods are accepted, considering the sensitivity and reliability for detection of genetically modified-derived material in routine analysis. In this paper, a review of currently available polymerase chain reaction methods for screening and quantifying genetically modifiedderived ingredients is presented, discussing their applicability and limitations
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spelling Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foodsAplicabilidade da metodologia de reação de polimerase em cadeia em tempo real na determinação do percentual de organismos geneticamente modificados em alimentosFoods genetically modifiedPolymerase chain reactionFood labelingAlimentos geneticamente modificadosReação de polimerase em cadeiaRotulagem de alimentosDetection of genetically modified organisms in the food chain is an important issue for all subjects involved in raw material control, food industry and distribution. Both labeling and traceability of genetically modified organisms are current issues that are considered for trade and regulation. Currently, labeling of genetically modified foods containing detectable transgenic material is required by the Brazilian legislation. The Brazilian government published the Decree nº 4.680 in April 2003, which requires labeling for all foods or food ingredients, with a stricter labeling threshold of 1%. Although polymerase chain reaction technology hassome limitations, the high sensitivity and specificity explain why it has been the first choice of most analytical laboratories interested in detection of genetically modified organisms and their derived products. Among the currently available methods, polymerase chain reaction-based methods are accepted, considering the sensitivity and reliability for detection of genetically modified-derived material in routine analysis. In this paper, a review of currently available polymerase chain reaction methods for screening and quantifying genetically modifiedderived ingredients is presented, discussing their applicability and limitationsA detecção de organismos geneticamente modificados na cadeia alimentar é um aspecto importante para todos os assuntos envolvidos no controle de matéria-prima, na indústria de alimentos e na distribuição. A rotulagem e a rastreabilidade de organismos geneticamente modificados são questões atuais que são consideradas para o comércio e a regulamentação. Atualmente, a rotulagem de alimentos processados contendo material transgênico detectável é exigida pela legislação brasileira. O governo brasileiro publicou Decreto nº 4.680 em abril de 2003, que exige rotulagem para todos os alimentos ou ingredientes de alimento, com o limite para rotulagem de 1%. Embora a tecnologia de reação em cadeia da polimerase tenha algumas limitações, a alta sensibilidade e especificidade explicam sua escolha por parte dos laboratórios interessados em realizar análises de detecção de organismos geneticamente modificados e seus derivados. Entre os métodos atualmente disponíveis, aqueles baseados na reação em cadeia da polimerase geralmente são aceitos, considerando a sensibilidade e a confiabilidade na detecção de material geneticamente modificado-derivado em análises de rotina. Neste artigo, apresenta-se uma revisão de métodos atualmente disponíveis baseados na reação em cadeia da polimerase para detecção, identificação e quantificação de organismos geneticamente modificados e seus derivados, discutindo sua aplicabilidade e suas limitações.Núcleo de Editoração – PUC-Campinas2023-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9561Brazilian Journal of Nutrition; Vol. 21 No. 1 (2008): Revista de NutriçãoRevista de Nutrição; Vol. 21 Núm. 1 (2008): Revista de NutriçãoRevista de Nutrição; v. 21 n. 1 (2008): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9561/6921Copyright (c) 2023 Natália Eudes Fagundes de BARROS, Edna Maria Morais OLIVEIRA, Victor Augustus MARINhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFagundes de BARROS, Natália Eudes Morais OLIVEIRA, Edna MariaMARIN, Victor Augustus 2023-09-01T16:58:43Zoai:ojs.periodicos.puc-campinas.edu.br:article/9561Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-09-01T16:58:43Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false
dc.title.none.fl_str_mv Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
Aplicabilidade da metodologia de reação de polimerase em cadeia em tempo real na determinação do percentual de organismos geneticamente modificados em alimentos
title Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
spellingShingle Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
Fagundes de BARROS, Natália Eudes
Foods genetically modified
Polymerase chain reaction
Food labeling
Alimentos geneticamente modificados
Reação de polimerase em cadeia
Rotulagem de alimentos
title_short Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
title_full Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
title_fullStr Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
title_full_unstemmed Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
title_sort Applicability of the real-time polymerase chain reaction based-methods in quantification of genetically modified organisms in foods
author Fagundes de BARROS, Natália Eudes
author_facet Fagundes de BARROS, Natália Eudes
Morais OLIVEIRA, Edna Maria
MARIN, Victor Augustus
author_role author
author2 Morais OLIVEIRA, Edna Maria
MARIN, Victor Augustus
author2_role author
author
dc.contributor.author.fl_str_mv Fagundes de BARROS, Natália Eudes
Morais OLIVEIRA, Edna Maria
MARIN, Victor Augustus
dc.subject.por.fl_str_mv Foods genetically modified
Polymerase chain reaction
Food labeling
Alimentos geneticamente modificados
Reação de polimerase em cadeia
Rotulagem de alimentos
topic Foods genetically modified
Polymerase chain reaction
Food labeling
Alimentos geneticamente modificados
Reação de polimerase em cadeia
Rotulagem de alimentos
description Detection of genetically modified organisms in the food chain is an important issue for all subjects involved in raw material control, food industry and distribution. Both labeling and traceability of genetically modified organisms are current issues that are considered for trade and regulation. Currently, labeling of genetically modified foods containing detectable transgenic material is required by the Brazilian legislation. The Brazilian government published the Decree nº 4.680 in April 2003, which requires labeling for all foods or food ingredients, with a stricter labeling threshold of 1%. Although polymerase chain reaction technology hassome limitations, the high sensitivity and specificity explain why it has been the first choice of most analytical laboratories interested in detection of genetically modified organisms and their derived products. Among the currently available methods, polymerase chain reaction-based methods are accepted, considering the sensitivity and reliability for detection of genetically modified-derived material in routine analysis. In this paper, a review of currently available polymerase chain reaction methods for screening and quantifying genetically modifiedderived ingredients is presented, discussing their applicability and limitations
publishDate 2023
dc.date.none.fl_str_mv 2023-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9561
url https://periodicos.puc-campinas.edu.br/nutricao/article/view/9561
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9561/6921
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
dc.source.none.fl_str_mv Brazilian Journal of Nutrition; Vol. 21 No. 1 (2008): Revista de Nutrição
Revista de Nutrição; Vol. 21 Núm. 1 (2008): Revista de Nutrição
Revista de Nutrição; v. 21 n. 1 (2008): Revista de Nutrição
1678-9865
reponame:Revista de Nutrição
instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron:PUC_CAMP
instname_str Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron_str PUC_CAMP
institution PUC_CAMP
reponame_str Revista de Nutrição
collection Revista de Nutrição
repository.name.fl_str_mv Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
repository.mail.fl_str_mv ||sbi.submissionrn@puc-campinas.edu.br
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