Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/7602 |
Resumo: | ObjectiveTo evaluate the b-hydroxy-b-methylbutyrate supplementation infl uence on the morphological and morphometric characteristics of the diaphragm muscle of rats and verify whether there are sex differences. MethodsExperimental study with 48 Wistar rats (24 of each sex) divided into 3 groups: Control Group: in which a daily diet with saline solution was offered; Experimental Group: the same amount of food from the Control group consumed in the previous day and 0.3g/kg of β-hydroxy-β-methylbutyrate; Ad libitum Experimental Group: ad libitum feeding with the same dose of the supplement. The analysis consisted of histomorphometry and classification in diaphragm muscle fiber area bands. The procedures occurred 30 days after the start of the experiment. Data were analyzed using the one-way ANOVA and Tukey tests (p<0.05). ResultsThere was no increase in the cross-sectional area of the diaphragm muscle fibers with the supplementation protocol employed and a similar histological pattern in both sexes. No significant changes were observed in muscle fiber size ranges in the supplemented groups, suggesting that there was no hypertrophy of muscle fibers. ConclusionThis study suggests that β-hydroxy-β-methylbutyrate supplementation does not cause changes in the morphological and morphometric characteristics of the diaphragm muscle, regardless of sex. |
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Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscleDiaphragmHistologyRats WistarSupplementary feedingObjectiveTo evaluate the b-hydroxy-b-methylbutyrate supplementation infl uence on the morphological and morphometric characteristics of the diaphragm muscle of rats and verify whether there are sex differences. MethodsExperimental study with 48 Wistar rats (24 of each sex) divided into 3 groups: Control Group: in which a daily diet with saline solution was offered; Experimental Group: the same amount of food from the Control group consumed in the previous day and 0.3g/kg of β-hydroxy-β-methylbutyrate; Ad libitum Experimental Group: ad libitum feeding with the same dose of the supplement. The analysis consisted of histomorphometry and classification in diaphragm muscle fiber area bands. The procedures occurred 30 days after the start of the experiment. Data were analyzed using the one-way ANOVA and Tukey tests (p<0.05). ResultsThere was no increase in the cross-sectional area of the diaphragm muscle fibers with the supplementation protocol employed and a similar histological pattern in both sexes. No significant changes were observed in muscle fiber size ranges in the supplemented groups, suggesting that there was no hypertrophy of muscle fibers. ConclusionThis study suggests that β-hydroxy-β-methylbutyrate supplementation does not cause changes in the morphological and morphometric characteristics of the diaphragm muscle, regardless of sex. Núcleo de Editoração – PUC-Campinas2023-03-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/7602Brazilian Journal of Nutrition; Vol. 32 (2019): Revista de NutriçãoRevista de Nutrição; Vol. 32 (2019): Revista de NutriçãoRevista de Nutrição; v. 32 (2019): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/7602/5141Copyright (c) 2023 Cleuber Rodrigo de Souza BUENO, Bruno MARTINELLI, Gabriele Candido BERNARDO, Leticia Rossi DARÉ, Jesus Carlos ANDREO, Geraldo Marco ROSA JUNIORhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessde Souza BUENO, Cleuber Rodrigo MARTINELLI, Bruno Candido BERNARDO, Gabriele Rossi DARÉ, LeticiaCarlos ANDREO, Jesus ROSA JUNIOR, Geraldo Marco2023-03-08T12:03:23Zoai:ojs.periodicos.puc-campinas.edu.br:article/7602Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-03-08T12:03:23Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
title |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
spellingShingle |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle de Souza BUENO, Cleuber Rodrigo Diaphragm Histology Rats Wistar Supplementary feeding |
title_short |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
title_full |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
title_fullStr |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
title_full_unstemmed |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
title_sort |
Thirty-day β-hydroxy-β-methylbutyrate supplementation with a controlled diet does not alter the morphological and morphometric characteristics of the rat diaphragm muscle |
author |
de Souza BUENO, Cleuber Rodrigo |
author_facet |
de Souza BUENO, Cleuber Rodrigo MARTINELLI, Bruno Candido BERNARDO, Gabriele Rossi DARÉ, Leticia Carlos ANDREO, Jesus ROSA JUNIOR, Geraldo Marco |
author_role |
author |
author2 |
MARTINELLI, Bruno Candido BERNARDO, Gabriele Rossi DARÉ, Leticia Carlos ANDREO, Jesus ROSA JUNIOR, Geraldo Marco |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
de Souza BUENO, Cleuber Rodrigo MARTINELLI, Bruno Candido BERNARDO, Gabriele Rossi DARÉ, Leticia Carlos ANDREO, Jesus ROSA JUNIOR, Geraldo Marco |
dc.subject.por.fl_str_mv |
Diaphragm Histology Rats Wistar Supplementary feeding |
topic |
Diaphragm Histology Rats Wistar Supplementary feeding |
description |
ObjectiveTo evaluate the b-hydroxy-b-methylbutyrate supplementation infl uence on the morphological and morphometric characteristics of the diaphragm muscle of rats and verify whether there are sex differences. MethodsExperimental study with 48 Wistar rats (24 of each sex) divided into 3 groups: Control Group: in which a daily diet with saline solution was offered; Experimental Group: the same amount of food from the Control group consumed in the previous day and 0.3g/kg of β-hydroxy-β-methylbutyrate; Ad libitum Experimental Group: ad libitum feeding with the same dose of the supplement. The analysis consisted of histomorphometry and classification in diaphragm muscle fiber area bands. The procedures occurred 30 days after the start of the experiment. Data were analyzed using the one-way ANOVA and Tukey tests (p<0.05). ResultsThere was no increase in the cross-sectional area of the diaphragm muscle fibers with the supplementation protocol employed and a similar histological pattern in both sexes. No significant changes were observed in muscle fiber size ranges in the supplemented groups, suggesting that there was no hypertrophy of muscle fibers. ConclusionThis study suggests that β-hydroxy-β-methylbutyrate supplementation does not cause changes in the morphological and morphometric characteristics of the diaphragm muscle, regardless of sex. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/7602 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/7602 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/7602/5141 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 32 (2019): Revista de Nutrição Revista de Nutrição; Vol. 32 (2019): Revista de Nutrição Revista de Nutrição; v. 32 (2019): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
_version_ |
1799126067805421568 |