Impact of vitamin C supplementation on lipid peroxidation and reduced glutathione levels in the liver tissue of mice with cyclophosphamide-induced immunosuppression

• Autor(a) principal: de Sousa ARAÚJO, Évelyn
• Data de Publicação: 2023
• Outros Autores: Scussel GARCIA, Rosane, DAMBRÓS, Betina, PIENIZ, Simone, SCHNEIDER, Augusto, Torres ABIB, Renata
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/8099
• topic: Ascorbic acid; Cyclophosphamide; Oxidative stress; Glutathione; Lipidperoxidation; Ácido ascórbico; Ciclofosfamida; Estresse oxidativo; Glutationa; Peroxidação de lipídeos

ObjectiveTo investigate the effects of vitamin C supplementation on the levels of lipid peroxidation and reduced glutathione in the liver tissue of mice immunosuppressed with cyclophosphamide. MethodsThirty-two 45-day-old female Swiss mice were divided into four groups of eight animals each as follows: control (distilled water); vitamin C (50 mg/kg); cyclophosphamide (100 + 150 mg/kg); and treatment (vitamin C 50 mg/kg + cyclophosphamide 100 +150 mg/kg). The substances were provided intraperitoneally for six days, and on the seventh day, the mice were euthanized. The biochemical analyses of lipid peroxidation (quantification of thiobarbituric acid-reactive substances) and reduced glutathione (estimate of non-protein thiols) were performed on liver tissue. ResultsCyclophosphamide increased the levels of lipid peroxidation (p<0.0001). Significant changes were not found in the groups treated with vitamin C. Cyclophosphamide alone did not affect the levels of reduced glutathione. Compared with the control group, vitamin C reduced the levels of reduced glutathione in animals that received or not cyclophosphamide. Vitamin C interacted with cyclophosphamide, that is, the chemotherapeutic agent further decreased the lower levels of reduced glutathione secondary to vitamin C intake. ConclusionCyclophosphamide, in the study dosage and duration, was capable of inducing oxidative damage, verified by increased lipid peroxidation. A vitamin C dosage of 50mg/kg of body weight did not protect against the oxidative damage caused by the chemotherapeutic agent.