Cytokine profile of rats fed a diet containing shrimp
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Nutrição |
Texto Completo: | https://periodicos.puc-campinas.edu.br/nutricao/article/view/8386 |
Resumo: | ObjectiveStudies have shown that shrimps reduced the tensile strength of scars in rat skin. The aim of the present study was to assess the cytokine profile of rats fed shrimp. MethodsGroup 1 (control) received a regular diet and Group 2 (experimental) received a diet containing 33% shrimp for nine days. The two diets contained the same amounts of proteins, fats and carbohydrates. Serum cytokine levels were determined by ELISA and a segment of the jejunum was taken to investigate its histological morphology and eosinophil infiltrate. ResultsThe experimental group had lower serum levels of interleukin-4 (IL-4) (14.4±1.9 versus 18.11±2.6pg/mL; p<0.05) and IL-10 (5.0±0.98 versus 7.5±1.2pg/mL; p<0.05) and higher levels of IL-6 (17.8±2.3 versus 3.2±0.4pg/mL, p<0.001) than controls. Morphologically, the shrimp-based diet caused an architectural disorganization of the intestinal mucosa and a greater amount of eosinophils in the jejunal villus. ConclusionOur data suggests that shrimp consumption leads to a significant increase in the cytokine IL-6, a decrease in the immunomodulatory cytokine IL-10 in the serum of rats, and high eosinophil infiltration in the jejunum. The cytokine profile typical of inflammation and the histological aspect of the jejunum are compatible with food allergy. |
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Cytokine profile of rats fed a diet containing shrimpPerfil das citocinas de ratos alimentados com dieta de camarãoCytokinesDietEosinophilsInterleukinsCitocinasDietaEosinófilosInterleucinasObjectiveStudies have shown that shrimps reduced the tensile strength of scars in rat skin. The aim of the present study was to assess the cytokine profile of rats fed shrimp. MethodsGroup 1 (control) received a regular diet and Group 2 (experimental) received a diet containing 33% shrimp for nine days. The two diets contained the same amounts of proteins, fats and carbohydrates. Serum cytokine levels were determined by ELISA and a segment of the jejunum was taken to investigate its histological morphology and eosinophil infiltrate. ResultsThe experimental group had lower serum levels of interleukin-4 (IL-4) (14.4±1.9 versus 18.11±2.6pg/mL; p<0.05) and IL-10 (5.0±0.98 versus 7.5±1.2pg/mL; p<0.05) and higher levels of IL-6 (17.8±2.3 versus 3.2±0.4pg/mL, p<0.001) than controls. Morphologically, the shrimp-based diet caused an architectural disorganization of the intestinal mucosa and a greater amount of eosinophils in the jejunal villus. ConclusionOur data suggests that shrimp consumption leads to a significant increase in the cytokine IL-6, a decrease in the immunomodulatory cytokine IL-10 in the serum of rats, and high eosinophil infiltration in the jejunum. The cytokine profile typical of inflammation and the histological aspect of the jejunum are compatible with food allergy.ObjetivoEstudos mostraram que a dieta suplementada com camarão reduziu a resistência cicatricial na pele de ratos. Nesse contexto, o objetivo do presente estudo foi avaliar o perfil das citocinas de ratos que receberam dieta adicionada com camarão. MétodosForam comparados um grupo controle e um grupo experimental, que receberam uma dieta enriquecida com camarão (33%) durante nove dias. As duas dietas continham quantidades semelhantes de proteínas, lipídeos, e carboidratos. Os níveis séricos de citocinas foram avaliados por ELISA, assim como um segmento de jejuno foi obtido para exame histológico da morfologia e infiltrado de eosinófilos. ResultadosA dieta adicionada com camarão diminuiu os níveis séricos de IL-4 (14,4±1,9 versus 18,11±2,6pg/mL, p<0,05) e IL-10 (5,0±0,98 versus 7,5±1,2pg/mL, p<0,05 e aumentou os níveis séricos de IL-6 (3,2±0,4 versus 17,8±2,3pg/mL, p<0,001) quando comparada com os animais controle. Morfologicamente, a dieta adicionada com camarão causou uma desorganização da arquitetura da mucosa intestinal, juntamente com uma abundância de eosinófilos nas vilosidades jejunais. ConclusãoOs dados sugerem que a ingestão de dieta adicionada com camarão leva a um aumento significativo da citocina IL-6, juntamente com uma diminuição da citocina imunomoduladora IL-10 no soro de ratos e um infiltrado de eosinófilos no jejuno. O padrão inflamatório das citocinas e o aspecto histológico do jejuno são compatíveis com alergia alimentar.Núcleo de Editoração – PUC-Campinas2023-04-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/8386Brazilian Journal of Nutrition; Vol. 26 No. 1 (2013): Revista de NutriçãoRevista de Nutrição; Vol. 26 Núm. 1 (2013): Revista de NutriçãoRevista de Nutrição; v. 26 n. 1 (2013): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPenghttps://periodicos.puc-campinas.edu.br/nutricao/article/view/8386/5900Copyright (c) 2023 Elizabeth Lage BORGES, Dirce Ribeiro de OLIVEIRA, Lucíola da Silva BARCELOS, Jorge Luiz PESQUEROhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLage BORGES, Elizabeth Ribeiro de OLIVEIRA, Dirceda Silva BARCELOS, Lucíola PESQUERO, Jorge Luiz 2023-10-05T19:11:32Zoai:ojs.periodicos.puc-campinas.edu.br:article/8386Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-10-05T19:11:32Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Cytokine profile of rats fed a diet containing shrimp Perfil das citocinas de ratos alimentados com dieta de camarão |
title |
Cytokine profile of rats fed a diet containing shrimp |
spellingShingle |
Cytokine profile of rats fed a diet containing shrimp Lage BORGES, Elizabeth Cytokines Diet Eosinophils Interleukins Citocinas Dieta Eosinófilos Interleucinas |
title_short |
Cytokine profile of rats fed a diet containing shrimp |
title_full |
Cytokine profile of rats fed a diet containing shrimp |
title_fullStr |
Cytokine profile of rats fed a diet containing shrimp |
title_full_unstemmed |
Cytokine profile of rats fed a diet containing shrimp |
title_sort |
Cytokine profile of rats fed a diet containing shrimp |
author |
Lage BORGES, Elizabeth |
author_facet |
Lage BORGES, Elizabeth Ribeiro de OLIVEIRA, Dirce da Silva BARCELOS, Lucíola PESQUERO, Jorge Luiz |
author_role |
author |
author2 |
Ribeiro de OLIVEIRA, Dirce da Silva BARCELOS, Lucíola PESQUERO, Jorge Luiz |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Lage BORGES, Elizabeth Ribeiro de OLIVEIRA, Dirce da Silva BARCELOS, Lucíola PESQUERO, Jorge Luiz |
dc.subject.por.fl_str_mv |
Cytokines Diet Eosinophils Interleukins Citocinas Dieta Eosinófilos Interleucinas |
topic |
Cytokines Diet Eosinophils Interleukins Citocinas Dieta Eosinófilos Interleucinas |
description |
ObjectiveStudies have shown that shrimps reduced the tensile strength of scars in rat skin. The aim of the present study was to assess the cytokine profile of rats fed shrimp. MethodsGroup 1 (control) received a regular diet and Group 2 (experimental) received a diet containing 33% shrimp for nine days. The two diets contained the same amounts of proteins, fats and carbohydrates. Serum cytokine levels were determined by ELISA and a segment of the jejunum was taken to investigate its histological morphology and eosinophil infiltrate. ResultsThe experimental group had lower serum levels of interleukin-4 (IL-4) (14.4±1.9 versus 18.11±2.6pg/mL; p<0.05) and IL-10 (5.0±0.98 versus 7.5±1.2pg/mL; p<0.05) and higher levels of IL-6 (17.8±2.3 versus 3.2±0.4pg/mL, p<0.001) than controls. Morphologically, the shrimp-based diet caused an architectural disorganization of the intestinal mucosa and a greater amount of eosinophils in the jejunal villus. ConclusionOur data suggests that shrimp consumption leads to a significant increase in the cytokine IL-6, a decrease in the immunomodulatory cytokine IL-10 in the serum of rats, and high eosinophil infiltration in the jejunum. The cytokine profile typical of inflammation and the histological aspect of the jejunum are compatible with food allergy. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8386 |
url |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8386 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.puc-campinas.edu.br/nutricao/article/view/8386/5900 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
publisher.none.fl_str_mv |
Núcleo de Editoração – PUC-Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Nutrition; Vol. 26 No. 1 (2013): Revista de Nutrição Revista de Nutrição; Vol. 26 Núm. 1 (2013): Revista de Nutrição Revista de Nutrição; v. 26 n. 1 (2013): Revista de Nutrição 1678-9865 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
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1799126069764161536 |