Effect of aqueous rosemary extract (Rosmarinus officinalis L.) on the oxidative stress of diabetic rats

• Autor(a) principal: SILVA, Ana Mara de Oliveira e
• Data de Publicação: 2023
• Outros Autores: ANDRADE-WARTHA, Elma Regina Silva de, CARVALHO, Eliane Bonifácio Teixeira de, LIMA, Alessandro de, NOVOA, Alexis Vidal, MANCINI-FILHO, Jorge
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9282
• topic: Antioxidants; Phenolic compounds; Mellitus diabetes; Enzymes; Rosemarinus; Antioxidantes; Compostos fenólicos; Diabetes mellitus; Enzimas; Rosmarinus

ObjectiveThis study assessed the effect of aqueous rosemary extract on the oxidative stress of diabetic rats.MethodsAqueous rosemary extract was obtained by the sequential method. Total phenolic content was determined by the Folin Ciocateau method and antioxidant activity in vitro was determined by the following methods: β-carotene/linoleic acid, 2,2 Difenil-1-Picril-hidrazil and oxygen radical absorbance capacity. Male Wistar rats were distributed into 5 groups: control, diabetic, and three groups of diabetic animals treated with aqueous rosemary extract in different concentrations: 25, 50 or 100mg/kg orally for 30 days. Diabetes was induced by streptozotocin and, at the end of the experiment, blood was collected to assess the percentage of glycated hemoglobin; liver and brain tissues were collected for the determination of the antioxidant enzymes: superoxide dismutase, catalase, glutatione peroxidase and glutatione reductase.ResultsAqueous rosemary extract contains high levels of phenolic compounds and significant total antioxidant activity in vitro according to the three methods. Aqueous rosemary extract at a concentration of 50mg/kg decreased the percentage of glycated hemoglobin and increased the activity of glutatiose peroxidase and catalase enzymes in the liver and superoxide dismutase in the brain of diabetic rats. However, no dose-response effect was observed in the other concentrations.ConclusionAqueous rosemary extract presented significant antioxidant capacity in vitro attributed to its high phenolic compound content. When administered to rats at a concentration of 50mg/kg, it proved to be efficient against the oxidative stress secondary to experimental diabetes.