Detection of Listeria, Salmonella and Klebsiella in a hospital food service

Detalhes bibliográficos
Autor(a) principal: PINTO, Uelinton Manoel
Data de Publicação: 2023
Outros Autores: Rezende CARDOSO, Rodrigo, Dantas VANETTI, Maria Cristina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Nutrição
Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9194
Resumo: ObjectiveThe purpose of the research was to investigate the presence of Listeria, Salmonella and Klebsiella on samples of enteral diets and utensils, surfaces and equipments involved in food preparation in a hospital food service. MethodsFifty samples collected from utensils, surfaces and equipment used for food preparation in a hospital food service, and four samples collected from enteral diets were tested for bacteria of genera Listeria, Salmonella and Klebsiella. Typical colonies of bacteria of the genus Listeria from enteral diet were isolated in Oxford agar and contamination by L. monocytogenes was confirmed by immunoanalysis. ResultsL. monocytogenes, S. rissen and Klebsiella were isolated from enteral diet. For their relevance as agents of hospital infections, bacteria of the genus Klebsiella were evaluated. K. pneumoniae were found in equipment and utensil, and K. oxytoca were found in environment, equipment and enteral diet samples. L. monocytogenes showed resistance to cefoxitin and all Klebsiella were resistant to amoxacillin and ampicillin. S. rissen showed susceptibility to all 13 antibiotics tested. ConclusionThe study showed that 11% of the analyzed samples were contaminated with, at least, one of the investigated pathogens. Such results reiterate the need for awareness and knowledge of effective hygienic procedures in the hospital food manipulation areas, in order to ensure patients’ safety. 
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spelling Detection of Listeria, Salmonella and Klebsiella in a hospital food serviceDetecção de Listeria, Salmonella e Klebsiella em serviço de alimentação hospitalarfood servicebacterial contaminationfoodborne pathogensantibiotic susceptibilityfood contaminationalimentação hospitalarcontaminação bacterianapatógenos alimentaressusceptibilidade a antibióticoscontaminação de alimentosObjectiveThe purpose of the research was to investigate the presence of Listeria, Salmonella and Klebsiella on samples of enteral diets and utensils, surfaces and equipments involved in food preparation in a hospital food service. MethodsFifty samples collected from utensils, surfaces and equipment used for food preparation in a hospital food service, and four samples collected from enteral diets were tested for bacteria of genera Listeria, Salmonella and Klebsiella. Typical colonies of bacteria of the genus Listeria from enteral diet were isolated in Oxford agar and contamination by L. monocytogenes was confirmed by immunoanalysis. ResultsL. monocytogenes, S. rissen and Klebsiella were isolated from enteral diet. For their relevance as agents of hospital infections, bacteria of the genus Klebsiella were evaluated. K. pneumoniae were found in equipment and utensil, and K. oxytoca were found in environment, equipment and enteral diet samples. L. monocytogenes showed resistance to cefoxitin and all Klebsiella were resistant to amoxacillin and ampicillin. S. rissen showed susceptibility to all 13 antibiotics tested. ConclusionThe study showed that 11% of the analyzed samples were contaminated with, at least, one of the investigated pathogens. Such results reiterate the need for awareness and knowledge of effective hygienic procedures in the hospital food manipulation areas, in order to ensure patients’ safety. ObjetivoA presença de Listeria monocytogenes, Salmonella sp. e Klebsiella sp. em dietas enterais e em ambientes, utensílios e equipamentos de preparo de alimentos em serviço de alimentação hospitalar, foi o objetivo desta pesquisa. MétodosA contaminação de ambientes, utensílios e equipamentos de preparo de alimentos em serviço de alimentação hospitalar por Listeria monocytogenes, Salmonella sp. e Klebsiella sp. foi avaliada em 50 amostras coletadas pela técnica de swab. Quatro amostras de dietas enterais também foram analisadas. Colônias típicas de bactérias do gênero Listeria foram isoladas de dieta enteral em ágar Oxford e a identificação da espécie L. monocytogenes foi feita por testes bioquímicos e imunológicos. ResultadosA presença de Salmonella foi detectada em dieta enteral e identificada como S. rissen por sorologia. Pela relevância como agente causador de infecções hospitalares, bactérias do gênero Klebsiella foram pesquisadas e isoladas em ágar seletivo MacConkey-inositol-carbenicilina. K. pneumoniae foi encontrada em equipamento e utensílio e K. oxytoca em ambiente, equipamento e dieta enteral. Os isolados de L. monocytogenes apresentaram resistência apenas ao antibiótico cefoxitina e os do gênero Klebsiella foram resistentes a ampicilina e amoxilina. S. rissen foi sensível aos 13 antibióticos avaliados. ConclusãoA contaminação de 11% das amostras analisadas com pelo menos um dos patógenos, alerta para a necessidade de implantação de um rigoroso sistema de controle de qualidade nas áreas de manipulação dos alimentos, a fim de aumentar a segurança alimentar dos pacientes hospitalizados.Núcleo de Editoração – PUC-Campinas2023-08-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9194Brazilian Journal of Nutrition; Vol. 17 No. 3 (2004): Revista de NutriçãoRevista de Nutrição; Vol. 17 Núm. 3 (2004): Revista de NutriçãoRevista de Nutrição; v. 17 n. 3 (2004): Revista de Nutrição1678-9865reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMPporhttps://periodicos.puc-campinas.edu.br/nutricao/article/view/9194/6577Copyright (c) 2023 Uelinton Manoel PINTO, Rodrigo Rezende CARDOSO, Maria Cristina Dantas VANETTIhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess PINTO, Uelinton ManoelRezende CARDOSO, Rodrigo Dantas VANETTI, Maria Cristina 2023-08-11T19:40:12Zoai:ojs.periodicos.puc-campinas.edu.br:article/9194Revistahttp://www.scielo.br/rnPRIhttps://periodicos.puc-campinas.edu.br/nutricao/oai||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2023-08-11T19:40:12Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false
dc.title.none.fl_str_mv Detection of Listeria, Salmonella and Klebsiella in a hospital food service
Detecção de Listeria, Salmonella e Klebsiella em serviço de alimentação hospitalar
title Detection of Listeria, Salmonella and Klebsiella in a hospital food service
spellingShingle Detection of Listeria, Salmonella and Klebsiella in a hospital food service
PINTO, Uelinton Manoel
food service
bacterial contamination
foodborne pathogens
antibiotic susceptibility
food contamination
alimentação hospitalar
contaminação bacteriana
patógenos alimentares
susceptibilidade a antibióticos
contaminação de alimentos
title_short Detection of Listeria, Salmonella and Klebsiella in a hospital food service
title_full Detection of Listeria, Salmonella and Klebsiella in a hospital food service
title_fullStr Detection of Listeria, Salmonella and Klebsiella in a hospital food service
title_full_unstemmed Detection of Listeria, Salmonella and Klebsiella in a hospital food service
title_sort Detection of Listeria, Salmonella and Klebsiella in a hospital food service
author PINTO, Uelinton Manoel
author_facet PINTO, Uelinton Manoel
Rezende CARDOSO, Rodrigo
Dantas VANETTI, Maria Cristina
author_role author
author2 Rezende CARDOSO, Rodrigo
Dantas VANETTI, Maria Cristina
author2_role author
author
dc.contributor.author.fl_str_mv PINTO, Uelinton Manoel
Rezende CARDOSO, Rodrigo
Dantas VANETTI, Maria Cristina
dc.subject.por.fl_str_mv food service
bacterial contamination
foodborne pathogens
antibiotic susceptibility
food contamination
alimentação hospitalar
contaminação bacteriana
patógenos alimentares
susceptibilidade a antibióticos
contaminação de alimentos
topic food service
bacterial contamination
foodborne pathogens
antibiotic susceptibility
food contamination
alimentação hospitalar
contaminação bacteriana
patógenos alimentares
susceptibilidade a antibióticos
contaminação de alimentos
description ObjectiveThe purpose of the research was to investigate the presence of Listeria, Salmonella and Klebsiella on samples of enteral diets and utensils, surfaces and equipments involved in food preparation in a hospital food service. MethodsFifty samples collected from utensils, surfaces and equipment used for food preparation in a hospital food service, and four samples collected from enteral diets were tested for bacteria of genera Listeria, Salmonella and Klebsiella. Typical colonies of bacteria of the genus Listeria from enteral diet were isolated in Oxford agar and contamination by L. monocytogenes was confirmed by immunoanalysis. ResultsL. monocytogenes, S. rissen and Klebsiella were isolated from enteral diet. For their relevance as agents of hospital infections, bacteria of the genus Klebsiella were evaluated. K. pneumoniae were found in equipment and utensil, and K. oxytoca were found in environment, equipment and enteral diet samples. L. monocytogenes showed resistance to cefoxitin and all Klebsiella were resistant to amoxacillin and ampicillin. S. rissen showed susceptibility to all 13 antibiotics tested. ConclusionThe study showed that 11% of the analyzed samples were contaminated with, at least, one of the investigated pathogens. Such results reiterate the need for awareness and knowledge of effective hygienic procedures in the hospital food manipulation areas, in order to ensure patients’ safety. 
publishDate 2023
dc.date.none.fl_str_mv 2023-08-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9194
url https://periodicos.puc-campinas.edu.br/nutricao/article/view/9194
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.puc-campinas.edu.br/nutricao/article/view/9194/6577
dc.rights.driver.fl_str_mv Copyright (c) 2023 Uelinton Manoel PINTO, Rodrigo Rezende CARDOSO, Maria Cristina Dantas VANETTI
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Uelinton Manoel PINTO, Rodrigo Rezende CARDOSO, Maria Cristina Dantas VANETTI
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
publisher.none.fl_str_mv Núcleo de Editoração – PUC-Campinas
dc.source.none.fl_str_mv Brazilian Journal of Nutrition; Vol. 17 No. 3 (2004): Revista de Nutrição
Revista de Nutrição; Vol. 17 Núm. 3 (2004): Revista de Nutrição
Revista de Nutrição; v. 17 n. 3 (2004): Revista de Nutrição
1678-9865
reponame:Revista de Nutrição
instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron:PUC_CAMP
instname_str Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
instacron_str PUC_CAMP
institution PUC_CAMP
reponame_str Revista de Nutrição
collection Revista de Nutrição
repository.name.fl_str_mv Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)
repository.mail.fl_str_mv ||sbi.submissionrn@puc-campinas.edu.br
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