In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics

Detalhes bibliográficos
Autor(a) principal: Pithon, Matheus Melo
Data de Publicação: 2008
Outros Autores: dos Santos, Rogério Lacerda, Ruellas, Antônio Carlos de Oliveira, Sant‘Anna, Eduardo Franzotti, Romanos, Maria Teresa Villela, Mendes, Gabriella da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3501
Resumo: Purpose: To evaluate the cytotoxicity of four different commercially marketed intermaxillary orthodontic elastics using cell culture. Methods: Four brands of orthodontic elastics were evaluated: American Orthodontics (Group1), TP Orthodontics (Group 2), Morelli (Group 3) and Uniden (Group 4). The elastics were previously sterilized using ultraviolet light and then placed on Petri dishes containing tissue cultures of HEp-2 cells at concentration of 105 for 24h. Five Petri dishes were used for each group, and a total of 15 elastics were tested per group (n=15). The cell monolayers were stained for evaluation of the diffusion halo and cell lysis according to the Response Index by Stanford. Results: There was absence of cytotoxicity for American Orthodontic and TP Orthodontic elastics, but high cytotoxicity produced by Morelli and Uniden elastics. Conclusion: Based on the methods used, the results suggest that Morelli and Uniden orthodontic elastics have high cytotoxicity. Key words: Cytotoxicity; orthodontic elastics; cell culture
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spelling In vitro cytotoxicity evaluation of intermaxillary orthodontic elasticsAvaliação in vitro da citotoxicidade de elásticos ortodônticos intermaxilaresCytotoxicityorthodontic elasticscell cultureCytotoxicity of orthodontic elasticsPurpose: To evaluate the cytotoxicity of four different commercially marketed intermaxillary orthodontic elastics using cell culture. Methods: Four brands of orthodontic elastics were evaluated: American Orthodontics (Group1), TP Orthodontics (Group 2), Morelli (Group 3) and Uniden (Group 4). The elastics were previously sterilized using ultraviolet light and then placed on Petri dishes containing tissue cultures of HEp-2 cells at concentration of 105 for 24h. Five Petri dishes were used for each group, and a total of 15 elastics were tested per group (n=15). The cell monolayers were stained for evaluation of the diffusion halo and cell lysis according to the Response Index by Stanford. Results: There was absence of cytotoxicity for American Orthodontic and TP Orthodontic elastics, but high cytotoxicity produced by Morelli and Uniden elastics. Conclusion: Based on the methods used, the results suggest that Morelli and Uniden orthodontic elastics have high cytotoxicity. Key words: Cytotoxicity; orthodontic elastics; cell cultureObjetivo: Avaliar a citotoxicidade de quatro diferentes marcas comerciais de elásticos intermaxilares intra-orais utilizando ensaio de citotoxicidade em culturas de células. Metodologia: Quatro marcas comerciais de elásticos foram avaliadas: American Orthodontics (Grupo 1), TP Orthodontics (Grupo 2), Morelli (Grupo3) e Uniden (Grupo 4). Os elásticos foram previamente esterilizados em luz ultravioleta e colocados em placas de Petri, as quais continham cultura de células HEp-2 em concentração de 105. Foram utilizadas cinco placas para cada grupo, num total de 15 elásticos testados por grupo (n=15). Após 24h, as monocamadas de células foram coradas para avaliação dos halos de difusão e de lise celular de acordo com o Índice de Resposta de Stanford. Resultados: Houve ausência de citotoxicidade para os elásticos das marcas American Orthodontic e TP Orthodontic e alta citotoxicidade nos elásticos das marcas Morelli e Uniden. Conclusão: Com base na metodologia utilizada e nos resultados encontrados, conclui-se que os elásticos da marca Morelli e Uniden apresentaram alta citotoxicidade. Palavras-chave: Citotoxicidade; elásticos ortodônticos; cultura de célulasEDIPUCRS - Editora Universitária da PUCRS2008-06-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleexperimental; in vitro; cell cultureapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3501Revista Odonto Ciência; Vol. 23 No. 3 (2008); 287 - 290Revista Odonto Ciência; v. 23 n. 3 (2008); 287 - 2901980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3501/3193Pithon, Matheus Melodos Santos, Rogério LacerdaRuellas, Antônio Carlos de OliveiraSant‘Anna, Eduardo FranzottiRomanos, Maria Teresa VillelaMendes, Gabriella da Silvainfo:eu-repo/semantics/openAccess2013-11-18T10:39:58Zoai:ojs.revistaseletronicas.pucrs.br:article/3501Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:39:58Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
Avaliação in vitro da citotoxicidade de elásticos ortodônticos intermaxilares
title In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
spellingShingle In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
Pithon, Matheus Melo
Cytotoxicity
orthodontic elastics
cell culture
Cytotoxicity of orthodontic elastics
title_short In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
title_full In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
title_fullStr In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
title_full_unstemmed In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
title_sort In vitro cytotoxicity evaluation of intermaxillary orthodontic elastics
author Pithon, Matheus Melo
author_facet Pithon, Matheus Melo
dos Santos, Rogério Lacerda
Ruellas, Antônio Carlos de Oliveira
Sant‘Anna, Eduardo Franzotti
Romanos, Maria Teresa Villela
Mendes, Gabriella da Silva
author_role author
author2 dos Santos, Rogério Lacerda
Ruellas, Antônio Carlos de Oliveira
Sant‘Anna, Eduardo Franzotti
Romanos, Maria Teresa Villela
Mendes, Gabriella da Silva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pithon, Matheus Melo
dos Santos, Rogério Lacerda
Ruellas, Antônio Carlos de Oliveira
Sant‘Anna, Eduardo Franzotti
Romanos, Maria Teresa Villela
Mendes, Gabriella da Silva
dc.subject.por.fl_str_mv Cytotoxicity
orthodontic elastics
cell culture
Cytotoxicity of orthodontic elastics
topic Cytotoxicity
orthodontic elastics
cell culture
Cytotoxicity of orthodontic elastics
description Purpose: To evaluate the cytotoxicity of four different commercially marketed intermaxillary orthodontic elastics using cell culture. Methods: Four brands of orthodontic elastics were evaluated: American Orthodontics (Group1), TP Orthodontics (Group 2), Morelli (Group 3) and Uniden (Group 4). The elastics were previously sterilized using ultraviolet light and then placed on Petri dishes containing tissue cultures of HEp-2 cells at concentration of 105 for 24h. Five Petri dishes were used for each group, and a total of 15 elastics were tested per group (n=15). The cell monolayers were stained for evaluation of the diffusion halo and cell lysis according to the Response Index by Stanford. Results: There was absence of cytotoxicity for American Orthodontic and TP Orthodontic elastics, but high cytotoxicity produced by Morelli and Uniden elastics. Conclusion: Based on the methods used, the results suggest that Morelli and Uniden orthodontic elastics have high cytotoxicity. Key words: Cytotoxicity; orthodontic elastics; cell culture
publishDate 2008
dc.date.none.fl_str_mv 2008-06-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
experimental; in vitro; cell culture
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3501
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3501/3193
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 23 No. 3 (2008); 287 - 290
Revista Odonto Ciência; v. 23 n. 3 (2008); 287 - 290
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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