Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista odonto ciência (Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249 |
Resumo: | Purpose: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes. Methods: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37 oC. The data were analyzed using Wilcoxon’s t test. Results: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01). Conclusion: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method. |
id |
PUC_RS-22_4dfc98844bd7632cb9441aff600eaf4e |
---|---|
oai_identifier_str |
oai:ojs.revistaseletronicas.pucrs.br:article/6249 |
network_acronym_str |
PUC_RS-22 |
network_name_str |
Revista odonto ciência (Online) |
repository_id_str |
|
spelling |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacyDesinfecção de moldes de hidrocolóide irreversível com vapor de hipoclorito de sódio: Avaliação da eficácia antimicrobianaDisinfectionhypochloriteirreversible hydrocolloidBiosecurityDesinfecçãohipoclorito de sódiohidroclóide irreversívelPurpose: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes. Methods: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37 oC. The data were analyzed using Wilcoxon’s t test. Results: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01). Conclusion: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method.Objetivo: avaliar a eficácia antimicrobiana do vapor de hipoclorito de sódio 2,5% e 5,25% na desinfecção de moldes de hidrocolóide irreversível em Caixa Umidificadora e Caixa Nebulizadora. Metodologia: Utilizaram-se 80 moldes de hemiarcos de pacientes, distribuídos em 4 grupos experimentais de 20 amostras cada, com respectivos controles. Os moldes permaneceram 10 min em atmosfera de hipoclorito de sódio com 100% de umidade relativa. Após desinfecção, cada molde foi imerso em soro fisiológico sob vibração ultrassônica e a análise microbiológica dessa solução foi realizada pela contagem de colônias que cresceram em meio de cultura BHI-ágar após 24h em estufa incubadora a 37oC. Os dados foram analisados pelo teste t de Wilcoxon. Resultados: Em todos os grupos observou-se maior número médio de colônias dos grupos controles em relação aos experimentais (P<0,0001). Para 2,5% de hipoclorito de sódio houve diferença estatística significativa entre Caixa Nebulizadora e Caixa Umidificadora. A concentração 5,25% não demonstrou diferença estatística entre os números médios de colônias nos dois métodos utilizados (P>0,01). Conclusão: Hipoclorito de sódio 5,25% poderá ser utilizado para desinfecção nos métodos Caixa Umidificadora e Caixa Nebulizadora, no entanto, a concentração 2,5%, só será eficaz quando utilizada no método Caixa Nebulizadora.EDIPUCRS - Editora Universitária da PUCRS2010-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249Revista Odonto Ciência; Vol. 25 No. 2 (2010); 182 - 187Revista Odonto Ciência; v. 25 n. 2 (2010); 182 - 1871980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249/5341Moura, Carmem Dolores V. Soares deMoura, Walter Leal deFrança, Fabiana Mantovani GomesMartins, Gregório Antonio SoaresFeltrim, Pedro PauloZanetti, Raquel Virgíniainfo:eu-repo/semantics/openAccess2013-11-11T12:39:32Zoai:ojs.revistaseletronicas.pucrs.br:article/6249Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T12:39:32Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy Desinfecção de moldes de hidrocolóide irreversível com vapor de hipoclorito de sódio: Avaliação da eficácia antimicrobiana |
title |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy |
spellingShingle |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy Moura, Carmem Dolores V. Soares de Disinfection hypochlorite irreversible hydrocolloid Biosecurity Desinfecção hipoclorito de sódio hidroclóide irreversível |
title_short |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy |
title_full |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy |
title_fullStr |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy |
title_full_unstemmed |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy |
title_sort |
Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy |
author |
Moura, Carmem Dolores V. Soares de |
author_facet |
Moura, Carmem Dolores V. Soares de Moura, Walter Leal de França, Fabiana Mantovani Gomes Martins, Gregório Antonio Soares Feltrim, Pedro Paulo Zanetti, Raquel Virgínia |
author_role |
author |
author2 |
Moura, Walter Leal de França, Fabiana Mantovani Gomes Martins, Gregório Antonio Soares Feltrim, Pedro Paulo Zanetti, Raquel Virgínia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moura, Carmem Dolores V. Soares de Moura, Walter Leal de França, Fabiana Mantovani Gomes Martins, Gregório Antonio Soares Feltrim, Pedro Paulo Zanetti, Raquel Virgínia |
dc.subject.por.fl_str_mv |
Disinfection hypochlorite irreversible hydrocolloid Biosecurity Desinfecção hipoclorito de sódio hidroclóide irreversível |
topic |
Disinfection hypochlorite irreversible hydrocolloid Biosecurity Desinfecção hipoclorito de sódio hidroclóide irreversível |
description |
Purpose: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes. Methods: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37 oC. The data were analyzed using Wilcoxon’s t test. Results: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01). Conclusion: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249 |
url |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249/5341 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
dc.source.none.fl_str_mv |
Revista Odonto Ciência; Vol. 25 No. 2 (2010); 182 - 187 Revista Odonto Ciência; v. 25 n. 2 (2010); 182 - 187 1980-6523 0102-9460 reponame:Revista odonto ciência (Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Revista odonto ciência (Online) |
collection |
Revista odonto ciência (Online) |
repository.name.fl_str_mv |
Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
||odontociencia@pucrs.br |
_version_ |
1754820875309285376 |