Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy

Detalhes bibliográficos
Autor(a) principal: Moura, Carmem Dolores V. Soares de
Data de Publicação: 2010
Outros Autores: Moura, Walter Leal de, França, Fabiana Mantovani Gomes, Martins, Gregório Antonio Soares, Feltrim, Pedro Paulo, Zanetti, Raquel Virgínia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249
Resumo: Purpose: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes. Methods: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37 oC. The data were analyzed using Wilcoxon’s t test. Results: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01). Conclusion: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method.
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spelling Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacyDesinfecção de moldes de hidrocolóide irreversível com vapor de hipoclorito de sódio: Avaliação da eficácia antimicrobianaDisinfectionhypochloriteirreversible hydrocolloidBiosecurityDesinfecçãohipoclorito de sódiohidroclóide irreversívelPurpose: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes. Methods: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37 oC. The data were analyzed using Wilcoxon’s t test. Results: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01). Conclusion: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method.Objetivo: avaliar a eficácia antimicrobiana do vapor de hipoclorito de sódio 2,5% e 5,25% na desinfecção de moldes de hidrocolóide irreversível em Caixa Umidificadora e Caixa Nebulizadora. Metodologia: Utilizaram-se 80 moldes de hemiarcos de pacientes, distribuídos em 4 grupos experimentais de 20 amostras cada, com respectivos controles. Os moldes permaneceram 10 min em atmosfera de hipoclorito de sódio com 100% de umidade relativa. Após desinfecção, cada molde foi imerso em soro fisiológico sob vibração ultrassônica e a análise microbiológica dessa solução foi realizada pela contagem de colônias que cresceram em meio de cultura BHI-ágar após 24h em estufa incubadora a 37oC. Os dados foram analisados pelo teste t de Wilcoxon. Resultados: Em todos os grupos observou-se maior número médio de colônias dos grupos controles em relação aos experimentais (P<0,0001). Para 2,5% de hipoclorito de sódio houve diferença estatística significativa entre Caixa Nebulizadora e Caixa Umidificadora. A concentração 5,25% não demonstrou diferença estatística entre os números médios de colônias nos dois métodos utilizados (P>0,01). Conclusão: Hipoclorito de sódio 5,25% poderá ser utilizado para desinfecção nos métodos Caixa Umidificadora e Caixa Nebulizadora, no entanto, a concentração 2,5%, só será eficaz quando utilizada no método Caixa Nebulizadora.EDIPUCRS - Editora Universitária da PUCRS2010-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249Revista Odonto Ciência; Vol. 25 No. 2 (2010); 182 - 187Revista Odonto Ciência; v. 25 n. 2 (2010); 182 - 1871980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249/5341Moura, Carmem Dolores V. Soares deMoura, Walter Leal deFrança, Fabiana Mantovani GomesMartins, Gregório Antonio SoaresFeltrim, Pedro PauloZanetti, Raquel Virgíniainfo:eu-repo/semantics/openAccess2013-11-11T12:39:32Zoai:ojs.revistaseletronicas.pucrs.br:article/6249Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T12:39:32Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
Desinfecção de moldes de hidrocolóide irreversível com vapor de hipoclorito de sódio: Avaliação da eficácia antimicrobiana
title Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
spellingShingle Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
Moura, Carmem Dolores V. Soares de
Disinfection
hypochlorite
irreversible hydrocolloid
Biosecurity
Desinfecção
hipoclorito de sódio
hidroclóide irreversível
title_short Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
title_full Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
title_fullStr Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
title_full_unstemmed Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
title_sort Disinfection of irreversible hydrocolloid impressions with sodium hypochlorite steam: Assessment of antimicrobial efficacy
author Moura, Carmem Dolores V. Soares de
author_facet Moura, Carmem Dolores V. Soares de
Moura, Walter Leal de
França, Fabiana Mantovani Gomes
Martins, Gregório Antonio Soares
Feltrim, Pedro Paulo
Zanetti, Raquel Virgínia
author_role author
author2 Moura, Walter Leal de
França, Fabiana Mantovani Gomes
Martins, Gregório Antonio Soares
Feltrim, Pedro Paulo
Zanetti, Raquel Virgínia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moura, Carmem Dolores V. Soares de
Moura, Walter Leal de
França, Fabiana Mantovani Gomes
Martins, Gregório Antonio Soares
Feltrim, Pedro Paulo
Zanetti, Raquel Virgínia
dc.subject.por.fl_str_mv Disinfection
hypochlorite
irreversible hydrocolloid
Biosecurity
Desinfecção
hipoclorito de sódio
hidroclóide irreversível
topic Disinfection
hypochlorite
irreversible hydrocolloid
Biosecurity
Desinfecção
hipoclorito de sódio
hidroclóide irreversível
description Purpose: To evaluate the antimicrobial effectiveness of 2.5% and 5.25% sodium hypochlorite steam to disinfect an irreversible hydrocolloid impressions in Humidifier and Nebulizer Boxes. Methods: The study used a total of 80 quadrant impressions of patients, divided into 4 experimental groups of 20 samples each, with respective controls. The impressions were placed in an atmosphere of sodium hypochlorite with 100% relative humidity for 10 min. After disinfection, each impression was immersed in saline solution that was ultrasonically vibrated. Microbiological analysis of the solution was carried out by counting colonies grown in BHI-agar culture medium after 24 hours in an incubator at 37 oC. The data were analyzed using Wilcoxon’s t test. Results: In all groups, it was found that the mean number of colonies in control groups was higher then in the experimental groups (P<0.0001). There was a significant difference between using the Nebulizer Box and the Humidifier Box when 2.5% sodium hypochlorite was used. At a concentration of 5.25% there was no statistical difference between the mean numbers of colonies for the two methods (P>0.01). Conclusion: Sodium hypochlorite at 5.25% can be used for disinfection in the Humidifier Box and Nebulizer Box methods. However, at a 2.5% concentration it is only effective in the Nebulizer box method.
publishDate 2010
dc.date.none.fl_str_mv 2010-04-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/6249/5341
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 25 No. 2 (2010); 182 - 187
Revista Odonto Ciência; v. 25 n. 2 (2010); 182 - 187
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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