Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity

Detalhes bibliográficos
Autor(a) principal: Moura, Carmem Dolores Vilarinho Soares
Data de Publicação: 2012
Outros Autores: Nogueira, Lorenna Bastos Lima Verde, Nascimento, Caroline Costa, Soares, Isadora Melo Vilarinho, Castro, Janaina Cordeiro de Oliveira, Moura, Walter Leal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/10497
Resumo: Purpose: The aim of this study is to assess the reduction of microbial load that adhered to irreversible hydrocolloid impressions obtained after rinsing with 0.12% and 0.2% chlorhexidine solutions. Methods: The study consisted of 24 participants (48 dental arch impressions) equally divided into two groups: Group 1 (0.12% chlorhexidine) and Group 2 (0.2% chlorhexidine). The impressions were taken using Hydrogun ® irreversible hydrocolloid before (control) and after (experimental) a single 10 mL mouthrinse of chlorhexidine for 1 minute. Each impression was placed in a sterile beaker containing 250 ml of saline. The microbiological analysis of the storage solution was carried out using BHI Agar culture medium. The plates were incubated for 24 hours, followed by a CFU (Colony Forming Units) count. The data were analyzed using the Wilcoxon’s t test. Results: There was a reduction in the degree of contamination of the impressions after rinsing with the antimicrobial mouth rinses at both concentrations when compared with the control group. There was a statistically significant difference when the effectiveness of the two concentrations used was compared. Conclusion: Therefore, using a chlorhexidine rinse prior to obtaining irreversible hydrocolloid impressions reduces contamination and is more efficient at the 0.2% concentration.
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spelling Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavityAvaliação microbiológica da eficácia do bochecho de clorexidina prévio a moldagem da caivdade bucalExposure to biological agentschlorhexidineirreversible hydrocolloidExposição a agentes biológicosclorexidinahidrocolóide irreversívelPurpose: The aim of this study is to assess the reduction of microbial load that adhered to irreversible hydrocolloid impressions obtained after rinsing with 0.12% and 0.2% chlorhexidine solutions. Methods: The study consisted of 24 participants (48 dental arch impressions) equally divided into two groups: Group 1 (0.12% chlorhexidine) and Group 2 (0.2% chlorhexidine). The impressions were taken using Hydrogun ® irreversible hydrocolloid before (control) and after (experimental) a single 10 mL mouthrinse of chlorhexidine for 1 minute. Each impression was placed in a sterile beaker containing 250 ml of saline. The microbiological analysis of the storage solution was carried out using BHI Agar culture medium. The plates were incubated for 24 hours, followed by a CFU (Colony Forming Units) count. The data were analyzed using the Wilcoxon’s t test. Results: There was a reduction in the degree of contamination of the impressions after rinsing with the antimicrobial mouth rinses at both concentrations when compared with the control group. There was a statistically significant difference when the effectiveness of the two concentrations used was compared. Conclusion: Therefore, using a chlorhexidine rinse prior to obtaining irreversible hydrocolloid impressions reduces contamination and is more efficient at the 0.2% concentration.Objetivo: Avaliar a redução da carga microbiana aderida nos moldes de hidrocolóide irreversível obtidos após bochecho com clorexidina nas concentrações 0,12% e 0,2%. Metodologia: O estudo incluiu 24 participantes (48 moldes de arcadas dentárias) divididos igualmente em dois grupos: Grupo 1 ( clorexidina 0,12%) e Grupo 2 (clorexidina 0,2%). As arcadas foram moldadas com hidrocolóide irreversível Hydrogun® antes (controle) e após (experimental) bochecho único com 10 mL de clorexidina nas concentrações específicas de cada grupo durante 1 min. Cada molde foi acomodado em bécker estéril contendo 250 mL de soro fisiológico. A partir dessa solução, realizou-se análise microbiológica em meio de cultura BHI Ágar. As placas foram submetidas a um período de incubação de 24 h, seguida de contagem das UFC (Unidades Formadoras de Colônias). Os dados foram submetidos à análise estatística aplicando o teste t de Wilcoxon. Resultados: Observou-se redução do grau de contaminação dos moldes obtidos após bochecho com o antimicrobiano nas duas concentrações em relação ao grupo controle e diferença estatística significativa quando comparadas a eficácia das duas concentrações utilizadas. Conclusão: O bochecho de clorexidina prévio à obtenção de moldes de hidrocolóide irreversível reduz a contaminação, com melhor eficácia na concentração 0,2%.EDIPUCRS - Editora Universitária da PUCRS2012-04-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlelaboratorial; experimentalapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/10497Revista Odonto Ciência; Vol. 27 No. 2 (2012); 156 - 160Revista Odonto Ciência; v. 27 n. 2 (2012); 156 - 1601980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/10497/8220Moura, Carmem Dolores Vilarinho SoaresNogueira, Lorenna Bastos Lima VerdeNascimento, Caroline CostaSoares, Isadora Melo VilarinhoCastro, Janaina Cordeiro de OliveiraMoura, Walter Lealinfo:eu-repo/semantics/openAccess2013-11-11T11:29:21Zoai:ojs.revistaseletronicas.pucrs.br:article/10497Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T11:29:21Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
Avaliação microbiológica da eficácia do bochecho de clorexidina prévio a moldagem da caivdade bucal
title Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
spellingShingle Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
Moura, Carmem Dolores Vilarinho Soares
Exposure to biological agents
chlorhexidine
irreversible hydrocolloid
Exposição a agentes biológicos
clorexidina
hidrocolóide irreversível
title_short Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
title_full Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
title_fullStr Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
title_full_unstemmed Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
title_sort Microbiological assessment of the efficacy of chlorhexidine mouthrinse before taking impression of the oral cavity
author Moura, Carmem Dolores Vilarinho Soares
author_facet Moura, Carmem Dolores Vilarinho Soares
Nogueira, Lorenna Bastos Lima Verde
Nascimento, Caroline Costa
Soares, Isadora Melo Vilarinho
Castro, Janaina Cordeiro de Oliveira
Moura, Walter Leal
author_role author
author2 Nogueira, Lorenna Bastos Lima Verde
Nascimento, Caroline Costa
Soares, Isadora Melo Vilarinho
Castro, Janaina Cordeiro de Oliveira
Moura, Walter Leal
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moura, Carmem Dolores Vilarinho Soares
Nogueira, Lorenna Bastos Lima Verde
Nascimento, Caroline Costa
Soares, Isadora Melo Vilarinho
Castro, Janaina Cordeiro de Oliveira
Moura, Walter Leal
dc.subject.por.fl_str_mv Exposure to biological agents
chlorhexidine
irreversible hydrocolloid
Exposição a agentes biológicos
clorexidina
hidrocolóide irreversível
topic Exposure to biological agents
chlorhexidine
irreversible hydrocolloid
Exposição a agentes biológicos
clorexidina
hidrocolóide irreversível
description Purpose: The aim of this study is to assess the reduction of microbial load that adhered to irreversible hydrocolloid impressions obtained after rinsing with 0.12% and 0.2% chlorhexidine solutions. Methods: The study consisted of 24 participants (48 dental arch impressions) equally divided into two groups: Group 1 (0.12% chlorhexidine) and Group 2 (0.2% chlorhexidine). The impressions were taken using Hydrogun ® irreversible hydrocolloid before (control) and after (experimental) a single 10 mL mouthrinse of chlorhexidine for 1 minute. Each impression was placed in a sterile beaker containing 250 ml of saline. The microbiological analysis of the storage solution was carried out using BHI Agar culture medium. The plates were incubated for 24 hours, followed by a CFU (Colony Forming Units) count. The data were analyzed using the Wilcoxon’s t test. Results: There was a reduction in the degree of contamination of the impressions after rinsing with the antimicrobial mouth rinses at both concentrations when compared with the control group. There was a statistically significant difference when the effectiveness of the two concentrations used was compared. Conclusion: Therefore, using a chlorhexidine rinse prior to obtaining irreversible hydrocolloid impressions reduces contamination and is more efficient at the 0.2% concentration.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
laboratorial; experimental
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/10497
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/10497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/10497/8220
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 27 No. 2 (2012); 156 - 160
Revista Odonto Ciência; v. 27 n. 2 (2012); 156 - 160
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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