Cytotoxicity of carbamide peroxide bleaching gel on L929 cells

• Autor(a) principal: Santos, Rogério Laceda
• Data de Publicação: 2010
• Outros Autores: Pithon, Matheus Melo, Martins, Fernanda Otaviano, Romanos, Maria Teresa Villela
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista odonto ciência (Online)
• Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5413
• topic: Toxicity; hydrogen peroxide; dental bleaching; Biocompatibility; Toxicidade; peróxido de hidrogênio; clareamento dentário; Biocompatibilidade

Purpose: To test the hypothesis that the higher the concentration of carbamide peroxide, the greater is its cytotoxicity to fibroblast cells. Methods: Three concentrations of carbamide peroxide (10%, 16%, and 22%) used in home bleaching techniques were evaluated regarding their cytotoxic effect on gingival tissues. The materials were divided into three groups as follows: Group C10 (White Gold Home 10%, Dentsply), Group C16 (White Gold Home 16%, Dentsply, and Group C22 (Nite White 22%, ACP Discus Dental). The cytotoxicity essay was carried out using cell cultures (mouse fibroblast L929 cell line) in which the viable cells were determined by means of the dye-uptake method performed at 2, 4, and 8 hours. Data were analyzed by analysis of variance (ANOVA) with multiple comparisons and Tukey’s test (P < 0.05). Results: The results showed statistically significant differences between Groups C10, C16, C22, and the cell control at 2, 4, and 8 hours (P < 0.05). The amount of cell lysis increased proportionally to the exposure time to the materials studied. Conclusion: The 22% carbamide peroxide group was more toxic than the other two groups (16% and 10% concentration) regardless of the exposure time.