Antibacterial action of red and green propolis extract in infected root canal

Detalhes bibliográficos
Autor(a) principal: Endo, Mônica Misaé
Data de Publicação: 2017
Outros Autores: Estrela, Cyntia R. A., Alencar, Ana Helena G., Silva, Júlio Almeida, Decurcio, Daniel Almeida, Estrela, Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/28930
Resumo: OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals.METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols - passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium.RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective.CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti inflammatory, antioxidant and low toxicity properties.
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spelling Antibacterial action of red and green propolis extract in infected root canalAção antimicrobiana do extrato de própolis vermelho e verde em canais radiculares infectadosEndodonticPropolisSodium hypochloriteEndodontiaPrópolisHipoclorito de sódioOBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals.METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols - passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium.RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective.CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti inflammatory, antioxidant and low toxicity properties.OBJETIVO: Investigar a ação antibacteriana do própolis vermelhos 30%, do própolis verde 40% e do hipoclorito de sódio 2,5% com protocolos de irrigação em canais radiculares infectados.MÉTODOS: Durante 60 dias, vinte e quatro canais radiculares foram inoculados com E. faecalis. Em todos os grupos experimentais foi realizado o preparo do canal radicular e dois protocolos de irrigação – irrigação ultra-sônica passiva e irrigação convencional. Nos grupos 1, 3, 5 e 7 foi feito o preparo do canal radicular associado à irrigação convencional com própolis 30%, própolis 40%, hipoclorito de sódio 2,5% e água destilada, respectivamente. Nos grupos 2, 4, 6 e 8 foi feito o preparo do canal radicular associado à irrigação ultra-sônica passiva com as soluções descritas acima. Os grupos 9 e 10 foram os controles (negativos e positivos). Amostras dos canais radiculares foram coletadas e imersas em 7 mL de BHI, durante 48 horas, a 37ºC. O crescimento bacteriano foi analisado por turvação do meio de cultura.RESULTADOS: Ação antibacteriana foi observada com própolis vermelho 30% e hipoclorito de sódio 2,5% quando utilizado irrigação ultra-sônica passiva e irrigação convencional após 20 minutos, porém, após 72 horas as substâncias não foram efetivas.CONCLUSÃO: Os agentes de irrigação e os protocolos não foram efetivos para eliminar os microorganismos dos canais radiculares infectados.EDIPUCRS - Editora Universitária da PUCRS2017-11-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlemicrobiology methodsapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2893010.15448/1980-6523.2017.2.28930Revista Odonto Ciência; Vol. 32 No. 2 (2017); 99-103Revista Odonto Ciência; v. 32 n. 2 (2017); 99-1031980-65230102-946010.15448/1980-6523.2017.2reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/28930/16178Copyright (c) 2017 Revista Odonto Ciênciainfo:eu-repo/semantics/openAccessEndo, Mônica MisaéEstrela, Cyntia R. A.Alencar, Ana Helena G.Silva, Júlio AlmeidaDecurcio, Daniel AlmeidaEstrela, Carlos2018-04-04T16:33:37Zoai:ojs.revistaseletronicas.pucrs.br:article/28930Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2018-04-04T16:33:37Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Antibacterial action of red and green propolis extract in infected root canal
Ação antimicrobiana do extrato de própolis vermelho e verde em canais radiculares infectados
title Antibacterial action of red and green propolis extract in infected root canal
spellingShingle Antibacterial action of red and green propolis extract in infected root canal
Endo, Mônica Misaé
Endodontic
Propolis
Sodium hypochlorite
Endodontia
Própolis
Hipoclorito de sódio
title_short Antibacterial action of red and green propolis extract in infected root canal
title_full Antibacterial action of red and green propolis extract in infected root canal
title_fullStr Antibacterial action of red and green propolis extract in infected root canal
title_full_unstemmed Antibacterial action of red and green propolis extract in infected root canal
title_sort Antibacterial action of red and green propolis extract in infected root canal
author Endo, Mônica Misaé
author_facet Endo, Mônica Misaé
Estrela, Cyntia R. A.
Alencar, Ana Helena G.
Silva, Júlio Almeida
Decurcio, Daniel Almeida
Estrela, Carlos
author_role author
author2 Estrela, Cyntia R. A.
Alencar, Ana Helena G.
Silva, Júlio Almeida
Decurcio, Daniel Almeida
Estrela, Carlos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Endo, Mônica Misaé
Estrela, Cyntia R. A.
Alencar, Ana Helena G.
Silva, Júlio Almeida
Decurcio, Daniel Almeida
Estrela, Carlos
dc.subject.por.fl_str_mv Endodontic
Propolis
Sodium hypochlorite
Endodontia
Própolis
Hipoclorito de sódio
topic Endodontic
Propolis
Sodium hypochlorite
Endodontia
Própolis
Hipoclorito de sódio
description OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals.METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols - passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium.RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective.CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti inflammatory, antioxidant and low toxicity properties.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
microbiology methods
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/28930
10.15448/1980-6523.2017.2.28930
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/28930
identifier_str_mv 10.15448/1980-6523.2017.2.28930
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/28930/16178
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista Odonto Ciência
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista Odonto Ciência
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 32 No. 2 (2017); 99-103
Revista Odonto Ciência; v. 32 n. 2 (2017); 99-103
1980-6523
0102-9460
10.15448/1980-6523.2017.2
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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