Antimicrobial action of root canal filling pastes used in deciduous teeth

Detalhes bibliográficos
Autor(a) principal: Vargas-Ferreira, Fabiana
Data de Publicação: 2009
Outros Autores: Angonese, Michele Pozzato, Friedrich, Helio Carvalho, Weiss, Rita Denise Niederauer, Friedrich, Rosane Salete, Praetzel, Juliana Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5557
Resumo: Purpose: This study assessed the antimicrobial effectiveness of an iodoform-based paste (Guedes-Pinto Paste, GPP) and a paste modified by the addition of a 2% chlorhexidine gluconate gel (CHX) to replace the camphorated parachlorophenol component of the original GPP. Methods: The antimicrobial action was tested against the following microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli and Bacillus subtilis. In the agar diffusion test, 18 Petri plates with 20 mL of BHIA were inoculated with 0.1 mL of the microbial suspensions. Paper disks were immersed in the experimental solutions for 1 min and were then placed over the BHIA surface in each agar plate. The plates were maintained for 1 h at room temperature and then incubated at 37ºC for 48 h. The diameters of the microbial inhibition halos were measured around the paper disks containing the substances. Data were analyzed using the Mann-Whitney U-test (α=0.05). Results: Antimicrobial action was observed for the GPP and CHX pastes, which presented absence of turbidity for almost all microorganisms. No statistically significant difference in the antimicrobial action was found between GPP and CHX. Conclusion: Both pastes present similar antimicrobial effectiveness against several microorganisms commonly found in endodontic infections in deciduous teeth.
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spelling Antimicrobial action of root canal filling pastes used in deciduous teethAção antimicrobiana de pastas obturadoras usadas em dentes decíduosPulpectomydeciduous teethchlorhexidinePurpose: This study assessed the antimicrobial effectiveness of an iodoform-based paste (Guedes-Pinto Paste, GPP) and a paste modified by the addition of a 2% chlorhexidine gluconate gel (CHX) to replace the camphorated parachlorophenol component of the original GPP. Methods: The antimicrobial action was tested against the following microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli and Bacillus subtilis. In the agar diffusion test, 18 Petri plates with 20 mL of BHIA were inoculated with 0.1 mL of the microbial suspensions. Paper disks were immersed in the experimental solutions for 1 min and were then placed over the BHIA surface in each agar plate. The plates were maintained for 1 h at room temperature and then incubated at 37ºC for 48 h. The diameters of the microbial inhibition halos were measured around the paper disks containing the substances. Data were analyzed using the Mann-Whitney U-test (α=0.05). Results: Antimicrobial action was observed for the GPP and CHX pastes, which presented absence of turbidity for almost all microorganisms. No statistically significant difference in the antimicrobial action was found between GPP and CHX. Conclusion: Both pastes present similar antimicrobial effectiveness against several microorganisms commonly found in endodontic infections in deciduous teeth.Objetivo: Este estudo avaliou a ação antimicrobiana de pastas, uma à base de iodofórmio conhecida por Pasta Guedes-Pinto (PGP) e outra modificada pela adição de digluconato de clorexidina (CHX) a 2% em substituição ao paramonoclorofenol canforado da formulação original da PGP. Metodologia: A ação antimicrobiana das duas pastas foi testada contra Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli e Bacillus subtilis. O método empregado foi o de Diluição em Meio Sólido, Difusão em Ágar. Dezoito placas de Petri com 20 mL de BHIA foram inoculados com 0,1 mL das suspensões microbianas. Discos de papel foram imersos nas soluções experimentais por 1 min e colocados sobre a superfície de BHIA em cada placa. As placas foram mantidas em temperatura ambiente por 1 h e então incubadas a 37ºC por 48 h. O diâmetro da inibição microbiana foi medida ao redor dos discos de papel. Os dados foram pelo teste U de Mann-Whitney (α=0,05). Resultados: A PGP teve ação bacteriostática contra todos os microrganismos e também bactericida exceto para Enterococcus faecalis e Bacillus subtilis. A CHX apresentou ação bacteriostática e bactericida contra todos os microrganismos. Não houve diferença estatística significante quanto à efetividade antimicrobiana entre as pastas avaliadas. Conclusão: Ambas as pastas apresentaram ação antimicrobiana contra quase todos os microrganismos encontrados em infecções endodônticas de dentes decíduos.EDIPUCRS - Editora Universitária da PUCRS2009-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5557Revista Odonto Ciência; Vol. 25 No. 1 (2010); 65 - 68Revista Odonto Ciência; v. 25 n. 1 (2010); 65 - 681980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5557/4864Vargas-Ferreira, FabianaAngonese, Michele PozzatoFriedrich, Helio CarvalhoWeiss, Rita Denise NiederauerFriedrich, Rosane SaletePraetzel, Juliana Rodriguesinfo:eu-repo/semantics/openAccess2013-11-11T12:48:37Zoai:ojs.revistaseletronicas.pucrs.br:article/5557Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T12:48:37Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Antimicrobial action of root canal filling pastes used in deciduous teeth
Ação antimicrobiana de pastas obturadoras usadas em dentes decíduos
title Antimicrobial action of root canal filling pastes used in deciduous teeth
spellingShingle Antimicrobial action of root canal filling pastes used in deciduous teeth
Vargas-Ferreira, Fabiana
Pulpectomy
deciduous teeth
chlorhexidine
title_short Antimicrobial action of root canal filling pastes used in deciduous teeth
title_full Antimicrobial action of root canal filling pastes used in deciduous teeth
title_fullStr Antimicrobial action of root canal filling pastes used in deciduous teeth
title_full_unstemmed Antimicrobial action of root canal filling pastes used in deciduous teeth
title_sort Antimicrobial action of root canal filling pastes used in deciduous teeth
author Vargas-Ferreira, Fabiana
author_facet Vargas-Ferreira, Fabiana
Angonese, Michele Pozzato
Friedrich, Helio Carvalho
Weiss, Rita Denise Niederauer
Friedrich, Rosane Salete
Praetzel, Juliana Rodrigues
author_role author
author2 Angonese, Michele Pozzato
Friedrich, Helio Carvalho
Weiss, Rita Denise Niederauer
Friedrich, Rosane Salete
Praetzel, Juliana Rodrigues
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Vargas-Ferreira, Fabiana
Angonese, Michele Pozzato
Friedrich, Helio Carvalho
Weiss, Rita Denise Niederauer
Friedrich, Rosane Salete
Praetzel, Juliana Rodrigues
dc.subject.por.fl_str_mv Pulpectomy
deciduous teeth
chlorhexidine
topic Pulpectomy
deciduous teeth
chlorhexidine
description Purpose: This study assessed the antimicrobial effectiveness of an iodoform-based paste (Guedes-Pinto Paste, GPP) and a paste modified by the addition of a 2% chlorhexidine gluconate gel (CHX) to replace the camphorated parachlorophenol component of the original GPP. Methods: The antimicrobial action was tested against the following microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli and Bacillus subtilis. In the agar diffusion test, 18 Petri plates with 20 mL of BHIA were inoculated with 0.1 mL of the microbial suspensions. Paper disks were immersed in the experimental solutions for 1 min and were then placed over the BHIA surface in each agar plate. The plates were maintained for 1 h at room temperature and then incubated at 37ºC for 48 h. The diameters of the microbial inhibition halos were measured around the paper disks containing the substances. Data were analyzed using the Mann-Whitney U-test (α=0.05). Results: Antimicrobial action was observed for the GPP and CHX pastes, which presented absence of turbidity for almost all microorganisms. No statistically significant difference in the antimicrobial action was found between GPP and CHX. Conclusion: Both pastes present similar antimicrobial effectiveness against several microorganisms commonly found in endodontic infections in deciduous teeth.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5557
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5557
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/5557/4864
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 25 No. 1 (2010); 65 - 68
Revista Odonto Ciência; v. 25 n. 1 (2010); 65 - 68
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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