Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS

Detalhes bibliográficos
Autor(a) principal: Queiroz, Celso Emanuel de Souza
Data de Publicação: 2008
Outros Autores: Pappen, Fernanda Geraldes, Lia, Rafael Comelli, Zanin, Fernando Perosa, Leonardo, Renato de Toledo, Carlos, Iracilda Zeponi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2892
Resumo: Purpose: Calcium hydroxide [Ca(OH)2] interaction with the immune system to destroy or neutralize bacteria and their by-products is not completely understood. This study evaluated the calcium hydroxide ability to neutralize Pseudomonas aeruginosa lipopolysaccharides (LPS) using two different methods: nitric oxide (NO) stimulation and Tumoral Necrosis Factoralpha (TNF-alpha) release in mice macrophage culture. Methods: For the NO assay, peritoneal exudate cells (PECs) were placed in contact with 25μg/mL and 50μg/mL LPS and LPS/Ca(OH)2 suspensions (50μg/25mg and 25μg/25mg). After incubation for 8h, Griess reagent was used, and the NO release was quantified. In the TNF-alpha assay the LPS solution was tested in 25μg/mL, and the LPS/Ca(OH)2 suspension was tested in 25μg/25mg concentration. After incubation for 24 hours, cells were fixed and stained with crystal violet. Absorbance values were obtained. Results were expressed in micromols. All tests were performed in triplicate. Results: The presence of Ca(OH)2 in both tested concentrations significantly reduced NO and TNF-alpha release. Conclusion: It can be concluded that bacteria LPS is a strong stimulus for NO and TNF-alpha release, but calcium hydroxide can neutralize it. Key words: Peritoneal macrophages; nitric oxide; lipopolysaccharides; tumor necrosis factor alpha
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spelling Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPSAção do Ca(OH)2 sobre a produção de TNF-alfa e NO de cultura de macrófagos estimulada por LPS de Pseudomonas aeruginosaPeritoneal macrophagesnitric oxidelipopolysaccharidestumor necrosis factoralphaCalcium hydroxidePurpose: Calcium hydroxide [Ca(OH)2] interaction with the immune system to destroy or neutralize bacteria and their by-products is not completely understood. This study evaluated the calcium hydroxide ability to neutralize Pseudomonas aeruginosa lipopolysaccharides (LPS) using two different methods: nitric oxide (NO) stimulation and Tumoral Necrosis Factoralpha (TNF-alpha) release in mice macrophage culture. Methods: For the NO assay, peritoneal exudate cells (PECs) were placed in contact with 25μg/mL and 50μg/mL LPS and LPS/Ca(OH)2 suspensions (50μg/25mg and 25μg/25mg). After incubation for 8h, Griess reagent was used, and the NO release was quantified. In the TNF-alpha assay the LPS solution was tested in 25μg/mL, and the LPS/Ca(OH)2 suspension was tested in 25μg/25mg concentration. After incubation for 24 hours, cells were fixed and stained with crystal violet. Absorbance values were obtained. Results were expressed in micromols. All tests were performed in triplicate. Results: The presence of Ca(OH)2 in both tested concentrations significantly reduced NO and TNF-alpha release. Conclusion: It can be concluded that bacteria LPS is a strong stimulus for NO and TNF-alpha release, but calcium hydroxide can neutralize it. Key words: Peritoneal macrophages; nitric oxide; lipopolysaccharides; tumor necrosis factor alphaObjetivo: A ação do hidróxido de cálcio [Ca(OH)2] com o sistema imune e o mecanismo de neutralização das bactérias e seus subprodutos ainda não foi completamente esclarecida. Neste estudo foi avaliada a capacidade do Ca(OH)2 em neutralizar o lipopolissacarídeo (LPS) de Pseudomonas aeruginosa, utilizando-se duas metodologias: liberação de Óxido Nítrico (NO) e Fator de Necrose Tumoral Alfa (TNF-alfa) em cultura de macrófagos peritoneais de camundongos. Metodologia: No ensaio do NO, as células peritoneais foram expostas a uma solução de LPS (25mg/mL e 50mg/mL); e à suspensão de LPS/Ca(OH)2 em duas concentrações (50mg/ 25mg e 25mg/25mg). Após 8 horas de incubação, foi utilizado reagente de Griess, e a liberação de NO foi quantificada. No ensaio do TNF-alfa, a solução de LPS foi usada na concentração de 25mg/mL e o LPS/Ca(OH)2 a 25mg/25mg. Após 24 horas, as células foram fixadas e coradas com cristal violeta, e os valores de absorbância foram obtidos. Os resultados foram expressos em micromols. Todos os testes foram realizados em triplicata. Resultados: A presença de Ca(OH)2 nas duas concentrações avaliadas reduziu significativamente a liberação de NO e TNF-alfa. Conclusão: Pode-se concluir que o LPS bacteriano representa um forte estímulo para liberação destas citocinas, mas o hidróxido de cálcio foi capaz de neutralizar este efeito. Palavras-chave: Macrófagos peritoneais; óxido nítrico; lipopolissacarídeos; fator de necrose tumoral alfaEDIPUCRS - Editora Universitária da PUCRS2008-07-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlelaboratory; cell cultureapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2892Revista Odonto Ciência; Vol. 23 No. 3 (2008); 225 - 228Revista Odonto Ciência; v. 23 n. 3 (2008); 225 - 2281980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2892/3180Queiroz, Celso Emanuel de SouzaPappen, Fernanda GeraldesLia, Rafael ComelliZanin, Fernando PerosaLeonardo, Renato de ToledoCarlos, Iracilda Zeponiinfo:eu-repo/semantics/openAccess2013-11-18T10:39:57Zoai:ojs.revistaseletronicas.pucrs.br:article/2892Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:39:57Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
Ação do Ca(OH)2 sobre a produção de TNF-alfa e NO de cultura de macrófagos estimulada por LPS de Pseudomonas aeruginosa
title Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
spellingShingle Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
Queiroz, Celso Emanuel de Souza
Peritoneal macrophages
nitric oxide
lipopolysaccharides
tumor necrosis factoralpha
Calcium hydroxide
title_short Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
title_full Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
title_fullStr Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
title_full_unstemmed Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
title_sort Ca(OH)2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS
author Queiroz, Celso Emanuel de Souza
author_facet Queiroz, Celso Emanuel de Souza
Pappen, Fernanda Geraldes
Lia, Rafael Comelli
Zanin, Fernando Perosa
Leonardo, Renato de Toledo
Carlos, Iracilda Zeponi
author_role author
author2 Pappen, Fernanda Geraldes
Lia, Rafael Comelli
Zanin, Fernando Perosa
Leonardo, Renato de Toledo
Carlos, Iracilda Zeponi
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Queiroz, Celso Emanuel de Souza
Pappen, Fernanda Geraldes
Lia, Rafael Comelli
Zanin, Fernando Perosa
Leonardo, Renato de Toledo
Carlos, Iracilda Zeponi
dc.subject.por.fl_str_mv Peritoneal macrophages
nitric oxide
lipopolysaccharides
tumor necrosis factoralpha
Calcium hydroxide
topic Peritoneal macrophages
nitric oxide
lipopolysaccharides
tumor necrosis factoralpha
Calcium hydroxide
description Purpose: Calcium hydroxide [Ca(OH)2] interaction with the immune system to destroy or neutralize bacteria and their by-products is not completely understood. This study evaluated the calcium hydroxide ability to neutralize Pseudomonas aeruginosa lipopolysaccharides (LPS) using two different methods: nitric oxide (NO) stimulation and Tumoral Necrosis Factoralpha (TNF-alpha) release in mice macrophage culture. Methods: For the NO assay, peritoneal exudate cells (PECs) were placed in contact with 25μg/mL and 50μg/mL LPS and LPS/Ca(OH)2 suspensions (50μg/25mg and 25μg/25mg). After incubation for 8h, Griess reagent was used, and the NO release was quantified. In the TNF-alpha assay the LPS solution was tested in 25μg/mL, and the LPS/Ca(OH)2 suspension was tested in 25μg/25mg concentration. After incubation for 24 hours, cells were fixed and stained with crystal violet. Absorbance values were obtained. Results were expressed in micromols. All tests were performed in triplicate. Results: The presence of Ca(OH)2 in both tested concentrations significantly reduced NO and TNF-alpha release. Conclusion: It can be concluded that bacteria LPS is a strong stimulus for NO and TNF-alpha release, but calcium hydroxide can neutralize it. Key words: Peritoneal macrophages; nitric oxide; lipopolysaccharides; tumor necrosis factor alpha
publishDate 2008
dc.date.none.fl_str_mv 2008-07-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
laboratory; cell culture
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2892
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2892
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2892/3180
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 23 No. 3 (2008); 225 - 228
Revista Odonto Ciência; v. 23 n. 3 (2008); 225 - 228
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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