Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction

Detalhes bibliográficos
Autor(a) principal: Marques, Bianca
Data de Publicação: 2024
Outros Autores: Nunes, Rafaela, Araújo-Rodrigues, Helena, Pintado, Manuela, Pereira, Ricardo N., Teixeira, José A., Rocha, Cristina M.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/88099
Resumo: Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investigated. Biomass was hydrolyzed using different temperatures (120210 °C), applying only water (sub-critical water; SCW) or water with a low concentration of alkali (0.1 mol L1 NaOH) and different reaction times (3090 min). Resultant hydrolysates were analyzed for crude and soluble protein, peptide profile, and bioactivity by combining protein quantification, antioxidant activity, and fast protein liquid chromatography measurements. Results showed that increasing temperature increases the degree of hydrolysis and that the addition of NaOH enhances the solubilization of peptides with high molecular weights. Also, hydrolysates showed interesting antioxidant activity, being 60 min the time of reaction with best antioxidant activity. Nevertheless, using only water (SCW) as solvent, without chemical additives, allows the delivering of interesting protein-based bioactive fractions. Sub-critical solvent treatment of porcine blood resulted in added-value fractions with potential bioactivities through a simple and environmentally friendly process.
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spelling Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extractionAutohydrolysisHydrothermal treatmentsProtein hydrolysisMeat by-productsCircular economyPork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investigated. Biomass was hydrolyzed using different temperatures (120210 °C), applying only water (sub-critical water; SCW) or water with a low concentration of alkali (0.1 mol L1 NaOH) and different reaction times (3090 min). Resultant hydrolysates were analyzed for crude and soluble protein, peptide profile, and bioactivity by combining protein quantification, antioxidant activity, and fast protein liquid chromatography measurements. Results showed that increasing temperature increases the degree of hydrolysis and that the addition of NaOH enhances the solubilization of peptides with high molecular weights. Also, hydrolysates showed interesting antioxidant activity, being 60 min the time of reaction with best antioxidant activity. Nevertheless, using only water (SCW) as solvent, without chemical additives, allows the delivering of interesting protein-based bioactive fractions. Sub-critical solvent treatment of porcine blood resulted in added-value fractions with potential bioactivities through a simple and environmentally friendly process.Open access funding provided by FCT|FCCN (b-on). This work was financed by the MobFood Mobilizing Project—“Mobilizing Scientific and Technological Knowledge in Response to Agri-Food Market Challenges” (POCI-01–0247-FEDER-024524•LISBOA-01–0247-FEDER-024524), financed by PORTUGAL2020, Lisb@a2020, COMPETE 2020 and the European Union, and by the project BIO ECONORTE—Water and nutrients management based on BIOrefinery and circular ECOnomy towards a sustainable agri-food system of the NORTE of Portugal (ref. NORTE-01–0145-FEDER-000070; financed by NORTE2020, Portugal 2020 and ESIF—European Structural and Investment Funds). This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of both the strategic funding of UIDB/04469/2020 unit and the FCT project UID/Multi/50016/2020. Ricardo N. Pereira acknowledges FCT for its Assistant Research program under the scope of Scientific Stimulus Employment with reference CEECIND/02903/2017info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoMarques, BiancaNunes, RafaelaAraújo-Rodrigues, HelenaPintado, ManuelaPereira, Ricardo N.Teixeira, José A.Rocha, Cristina M.R.20242024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/88099engMarques, Bianca; Nunes, R.; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R., Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction. Food and Bioprocess Technology, 17, 123-137, 20241935-513010.1007/s11947-023-03111-3http://www.springer.com/food+science/journal/11947info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-24T01:23:34Zoai:repositorium.sdum.uminho.pt:1822/88099Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:52:15.919727Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
title Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
spellingShingle Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
Marques, Bianca
Autohydrolysis
Hydrothermal treatments
Protein hydrolysis
Meat by-products
Circular economy
title_short Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
title_full Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
title_fullStr Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
title_full_unstemmed Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
title_sort Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction
author Marques, Bianca
author_facet Marques, Bianca
Nunes, Rafaela
Araújo-Rodrigues, Helena
Pintado, Manuela
Pereira, Ricardo N.
Teixeira, José A.
Rocha, Cristina M.R.
author_role author
author2 Nunes, Rafaela
Araújo-Rodrigues, Helena
Pintado, Manuela
Pereira, Ricardo N.
Teixeira, José A.
Rocha, Cristina M.R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Marques, Bianca
Nunes, Rafaela
Araújo-Rodrigues, Helena
Pintado, Manuela
Pereira, Ricardo N.
Teixeira, José A.
Rocha, Cristina M.R.
dc.subject.por.fl_str_mv Autohydrolysis
Hydrothermal treatments
Protein hydrolysis
Meat by-products
Circular economy
topic Autohydrolysis
Hydrothermal treatments
Protein hydrolysis
Meat by-products
Circular economy
description Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investigated. Biomass was hydrolyzed using different temperatures (120210 °C), applying only water (sub-critical water; SCW) or water with a low concentration of alkali (0.1 mol L1 NaOH) and different reaction times (3090 min). Resultant hydrolysates were analyzed for crude and soluble protein, peptide profile, and bioactivity by combining protein quantification, antioxidant activity, and fast protein liquid chromatography measurements. Results showed that increasing temperature increases the degree of hydrolysis and that the addition of NaOH enhances the solubilization of peptides with high molecular weights. Also, hydrolysates showed interesting antioxidant activity, being 60 min the time of reaction with best antioxidant activity. Nevertheless, using only water (SCW) as solvent, without chemical additives, allows the delivering of interesting protein-based bioactive fractions. Sub-critical solvent treatment of porcine blood resulted in added-value fractions with potential bioactivities through a simple and environmentally friendly process.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/88099
url https://hdl.handle.net/1822/88099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marques, Bianca; Nunes, R.; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R., Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction. Food and Bioprocess Technology, 17, 123-137, 2024
1935-5130
10.1007/s11947-023-03111-3
http://www.springer.com/food+science/journal/11947
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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