Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/5451 |
Resumo: | Background: Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in Troponin T (TnT), a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied. Results: In the present study five cDNAs for halibut TnT genes were cloned; three were splice variants arising from a single fast TnT (fTnT) gene; a fourth encoded a novel teleost specific fTnTlike cDNA (AfTnT) expressed exclusively in slow muscle and the fifth encoded the teleost specific sTnT2. THs modified the expression of halibut fTnT isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, AfTnT and sTnT2, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of TnT genes and muscle fibre organization occurred in halibut musculature. Conclusion: Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in TnT gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints. |
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Troponin T isoform expression is modulated during Atlantic Halibut metamorphosisTroponin TMetamorphosiBackground: Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in Troponin T (TnT), a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied. Results: In the present study five cDNAs for halibut TnT genes were cloned; three were splice variants arising from a single fast TnT (fTnT) gene; a fourth encoded a novel teleost specific fTnTlike cDNA (AfTnT) expressed exclusively in slow muscle and the fifth encoded the teleost specific sTnT2. THs modified the expression of halibut fTnT isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, AfTnT and sTnT2, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of TnT genes and muscle fibre organization occurred in halibut musculature. Conclusion: Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in TnT gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints.This work was carried out within the project Q5RS-2002-01192 with financial support from the Commission of the European Communities. However, it does not necessarily reflect the Commission's views and in no way anticipates its future policy in this area. This project was further co-financed by POCI 2010 and the European social funds attributed by the Portuguese National Science Foundation (FCT) to a Pluriannual project to CCMAR and a PhD fellowship to MAC (SFRH/BD/6133/2001).This work was carried out within the project Q5RS-2002-01192 with financial support from the Commission of the European Communities. However, it does not necessarily reflect the Commission's views and in no way anticipates its future policy in this area. This project was further co-financed by POCI 2010 and the European social funds attributed by the Portuguese National Science Foundation (FCT) to a Pluriannual project to CCMAR and a PhD fellowship to MAC (SFRH/BD/6133/2001).BMCSapientiaCampinho, M. A.Silva, NadiaNowell, MariLlewellyn, LyndaSweeney, Glen E.Power, Deborah2014-10-24T09:17:48Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/5451engMarco A Campinho, Nádia Silva, Mari A Nowell, Lynda Llewellyn, Glen E Sweeney and Deborah M Power, "Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis" in BMC Developmental Biology 2007, 7:711471-2148AUT: DPO00386;http://dx.doi.org/ 10.1186/1471-213X-7-71info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:43Zoai:sapientia.ualg.pt:10400.1/5451Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:31.881463Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
title |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
spellingShingle |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis Campinho, M. A. Troponin T Metamorphosi |
title_short |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
title_full |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
title_fullStr |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
title_full_unstemmed |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
title_sort |
Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis |
author |
Campinho, M. A. |
author_facet |
Campinho, M. A. Silva, Nadia Nowell, Mari Llewellyn, Lynda Sweeney, Glen E. Power, Deborah |
author_role |
author |
author2 |
Silva, Nadia Nowell, Mari Llewellyn, Lynda Sweeney, Glen E. Power, Deborah |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Campinho, M. A. Silva, Nadia Nowell, Mari Llewellyn, Lynda Sweeney, Glen E. Power, Deborah |
dc.subject.por.fl_str_mv |
Troponin T Metamorphosi |
topic |
Troponin T Metamorphosi |
description |
Background: Flatfish metamorphosis is a thyroid hormone (TH) driven process which leads to a dramatic change from a symmetrical larva to an asymmetrical juvenile. The effect of THs on muscle and in particular muscle sarcomer protein genes is largely unexplored in fish. The change in Troponin T (TnT), a pivotal protein in the assembly of skeletal muscles sarcomeres and a modulator of calcium driven muscle contraction, during flatfish metamophosis is studied. Results: In the present study five cDNAs for halibut TnT genes were cloned; three were splice variants arising from a single fast TnT (fTnT) gene; a fourth encoded a novel teleost specific fTnTlike cDNA (AfTnT) expressed exclusively in slow muscle and the fifth encoded the teleost specific sTnT2. THs modified the expression of halibut fTnT isoforms which changed from predominantly basic to acidic isoforms during natural and T4 induced metamorphosis. In contrast, expression of red muscle specific genes, AfTnT and sTnT2, did not change during natural metamorphosis or after T4 treatment. Prior to and after metamorphosis no change in the dorso-ventral symmetry or temporal-spatial expression pattern of TnT genes and muscle fibre organization occurred in halibut musculature. Conclusion: Muscle organisation in halibut remains symmetrical even after metamorphosis suggesting TH driven changes are associated with molecular adaptations. We hypothesize that species specific differences in TnT gene expression in teleosts underlies different larval muscle developmental programs which better adapts them to the specific ecological constraints. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2007-01-01T00:00:00Z 2014-10-24T09:17:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/5451 |
url |
http://hdl.handle.net/10400.1/5451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marco A Campinho, Nádia Silva, Mari A Nowell, Lynda Llewellyn, Glen E Sweeney and Deborah M Power, "Troponin T isoform expression is modulated during Atlantic Halibut metamorphosis" in BMC Developmental Biology 2007, 7:71 1471-2148 AUT: DPO00386; http://dx.doi.org/ 10.1186/1471-213X-7-71 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
BMC |
publisher.none.fl_str_mv |
BMC |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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