Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)

Detalhes bibliográficos
Autor(a) principal: Galay-Burgos, M.
Data de Publicação: 2007
Outros Autores: Power, Deborah, Llewellyn, Lynda, Sweeney, Glen E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/5486
Resumo: Flatfish such as the Atlantic halibut (Hippoglossus hippoglossus) undergo a dramatic metamorphosis that transforms the pelagic, symmetric larva into a benthic, cranially asymmetric juvenile. In common with amphibian metamorphosis, flatfish metamorphosis is under endocrine control with thyroid hormones being particularly important. In this report we confirm that tri-iodothyronine (T3) levels peak at metamorphic climax during halibut metamorphosis. Moreover we have isolated cDNA clones of TR and TR genes and confirmed the presence in halibut of two TR isoforms (representing the products of distinct genes) and two TR isoforms (generated from a single gene by alternative splicing). Real time PCR was used to assess expression of these genes during metamorphosis. TR shows the most dramatic expression profile, with a peak occurring during metamorphic climax.
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spelling Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)Flatfish such as the Atlantic halibut (Hippoglossus hippoglossus) undergo a dramatic metamorphosis that transforms the pelagic, symmetric larva into a benthic, cranially asymmetric juvenile. In common with amphibian metamorphosis, flatfish metamorphosis is under endocrine control with thyroid hormones being particularly important. In this report we confirm that tri-iodothyronine (T3) levels peak at metamorphic climax during halibut metamorphosis. Moreover we have isolated cDNA clones of TR and TR genes and confirmed the presence in halibut of two TR isoforms (representing the products of distinct genes) and two TR isoforms (generated from a single gene by alternative splicing). Real time PCR was used to assess expression of these genes during metamorphosis. TR shows the most dramatic expression profile, with a peak occurring during metamorphic climax.This work has been carried out within the project “Arrested development: The Molecular and Endocrine Basis of Flatfish Metamorphosis” (Q5RS-2002-01192) with financial support from the Commission of the European Communities. However, it does not necessarily reflect the Commission’s views and in no way anticipates its future policy in this area. We thank Heiddis Smáradóttir (Fiskeldi Eyjafjarðar, IS-600 Akureyri, Iceland) for collecting and providing the Atlantic halibut samples, and Karin Pittman and Øystein Sæle (both from the Department of Biology, University of Bergen, Norway) for analysing samples to determine developmental stage. We are also grateful to Marco Campinho for preparing the RNA used in the study.ElsevierSapientiaGalay-Burgos, M.Power, DeborahLlewellyn, LyndaSweeney, Glen E.2014-10-24T13:03:42Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/5486engMalyka Galay-Burgos, Deborah M. Power, Lynda Llewellyn, Glen E. Sweeney, "Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)" in Molecular and Cellular Endocrinology 281, 1-2 (2007) 56.0303-7207AUT: DPO00386;http://dx.doi.org/10.1016/j.mce.2007.10.009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:43Zoai:sapientia.ualg.pt:10400.1/5486Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:31.690538Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
title Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
spellingShingle Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
Galay-Burgos, M.
title_short Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
title_full Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
title_fullStr Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
title_full_unstemmed Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
title_sort Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)
author Galay-Burgos, M.
author_facet Galay-Burgos, M.
Power, Deborah
Llewellyn, Lynda
Sweeney, Glen E.
author_role author
author2 Power, Deborah
Llewellyn, Lynda
Sweeney, Glen E.
author2_role author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Galay-Burgos, M.
Power, Deborah
Llewellyn, Lynda
Sweeney, Glen E.
description Flatfish such as the Atlantic halibut (Hippoglossus hippoglossus) undergo a dramatic metamorphosis that transforms the pelagic, symmetric larva into a benthic, cranially asymmetric juvenile. In common with amphibian metamorphosis, flatfish metamorphosis is under endocrine control with thyroid hormones being particularly important. In this report we confirm that tri-iodothyronine (T3) levels peak at metamorphic climax during halibut metamorphosis. Moreover we have isolated cDNA clones of TR and TR genes and confirmed the presence in halibut of two TR isoforms (representing the products of distinct genes) and two TR isoforms (generated from a single gene by alternative splicing). Real time PCR was used to assess expression of these genes during metamorphosis. TR shows the most dramatic expression profile, with a peak occurring during metamorphic climax.
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
2014-10-24T13:03:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/5486
url http://hdl.handle.net/10400.1/5486
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Malyka Galay-Burgos, Deborah M. Power, Lynda Llewellyn, Glen E. Sweeney, "Thyroid hormone receptor expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus)" in Molecular and Cellular Endocrinology 281, 1-2 (2007) 56.
0303-7207
AUT: DPO00386;
http://dx.doi.org/10.1016/j.mce.2007.10.009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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