A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/51846 |
Resumo: | The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater produced interest in its use for sentinel surveillance at a community level and as a complementary approach to syndromic surveillance. With this work, we set the foundations for wastewater-based epidemiology (WBE) in Portugal by monitoring the trends of SARS-CoV-2 RNA circulation in the community, on a nationwide perspective during different epidemiological phases of the pandemic. The Charité assays (E_Sarbecco, RdRP, and N_Sarbecco) were applied to monitor, over 32-weeks (April to December 2020), the dynamics of SARS-CoV-2 RNA at the inlet of five wastewater treatment plants (WWTP), which together serve more than two million people in Portugal. Raw wastewater from three Coronavirus disease 2019 (COVID-19) reference hospitals was also analyzed during this period. In total, more than 600 samples were tested. For the first weeks, detection of SARS-CoV-2 RNA was sporadic, with concentrations varying from 103 to 105 genome copies per liter (GC/L). Prevalence of SARS-CoV-2 RNA increased steeply by the end of May into late June, mainly in Lisboa e Vale do Tejo region (LVT), during the reopening phase. After the summer, with the reopening of schools in mid-September and return to partial face-to-face work, a pronounced increase of SARS-CoV-2 RNA in wastewater was detected. In the LVT area, SARS-CoV-2 RNA load agreed with reported trends in hotspots of infection. Synchrony between trends of SARS-CoV-2 RNA in raw wastewater and daily new COVID-19 cases highlights the value of WBE as a surveillance tool, particularly after the phasing out of the epidemiological curve and when hotspots of disease re-emerge in the population which might be difficult to spot based solely on syndromic surveillance and contact tracing. This is the first study crossing several epidemiological stages highlighting the long-term use of WBE for SARS-CoV-2. |
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A wastewater-based epidemiology tool for COVID-19 surveillance in PortugalThe presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater produced interest in its use for sentinel surveillance at a community level and as a complementary approach to syndromic surveillance. With this work, we set the foundations for wastewater-based epidemiology (WBE) in Portugal by monitoring the trends of SARS-CoV-2 RNA circulation in the community, on a nationwide perspective during different epidemiological phases of the pandemic. The Charité assays (E_Sarbecco, RdRP, and N_Sarbecco) were applied to monitor, over 32-weeks (April to December 2020), the dynamics of SARS-CoV-2 RNA at the inlet of five wastewater treatment plants (WWTP), which together serve more than two million people in Portugal. Raw wastewater from three Coronavirus disease 2019 (COVID-19) reference hospitals was also analyzed during this period. In total, more than 600 samples were tested. For the first weeks, detection of SARS-CoV-2 RNA was sporadic, with concentrations varying from 103 to 105 genome copies per liter (GC/L). Prevalence of SARS-CoV-2 RNA increased steeply by the end of May into late June, mainly in Lisboa e Vale do Tejo region (LVT), during the reopening phase. After the summer, with the reopening of schools in mid-September and return to partial face-to-face work, a pronounced increase of SARS-CoV-2 RNA in wastewater was detected. In the LVT area, SARS-CoV-2 RNA load agreed with reported trends in hotspots of infection. Synchrony between trends of SARS-CoV-2 RNA in raw wastewater and daily new COVID-19 cases highlights the value of WBE as a surveillance tool, particularly after the phasing out of the epidemiological curve and when hotspots of disease re-emerge in the population which might be difficult to spot based solely on syndromic surveillance and contact tracing. This is the first study crossing several epidemiological stages highlighting the long-term use of WBE for SARS-CoV-2.ElsevierRepositório da Universidade de LisboaMonteiro, SílviaRente, DanielaCunha, Mónica V.Gomes, Manuel CarmoMarques, Tiago A.Lourenço, Artur B.Cardoso, EugéniaÁlvaro, PedroSilva, MarcoCoelho, NorbertaVilaça, JoãoMeireles, FátimaBrôco, NunoCarvalho, MartaSantos, Ricardo2024-01-01T01:31:05Z2022-012022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51846engSílvia Monteiro, Daniela Rente, Mónica V. Cunha, Manuel Carmo Gomes, Tiago A. Marques, Artur B. Lourenço, Eugénia Cardoso, Pedro Álvaro, Marco Silva, Norberta Coelho, João Vilaça, Fátima Meireles, Nuno Brôco, Marta Carvalho, Ricardo Santos, A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal, Science of The Total Environment, Volume 804, 2022, 150264, ISSN 0048-9697, https://doi.org/10.1016/j.scitotenv.2021.150264.10.1016/j.scitotenv.2021.150264info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-08T01:16:37Zoai:repositorio.ul.pt:10451/51846Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:03:01.107883Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
title |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
spellingShingle |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal Monteiro, Sílvia |
title_short |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
title_full |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
title_fullStr |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
title_full_unstemmed |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
title_sort |
A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal |
author |
Monteiro, Sílvia |
author_facet |
Monteiro, Sílvia Rente, Daniela Cunha, Mónica V. Gomes, Manuel Carmo Marques, Tiago A. Lourenço, Artur B. Cardoso, Eugénia Álvaro, Pedro Silva, Marco Coelho, Norberta Vilaça, João Meireles, Fátima Brôco, Nuno Carvalho, Marta Santos, Ricardo |
author_role |
author |
author2 |
Rente, Daniela Cunha, Mónica V. Gomes, Manuel Carmo Marques, Tiago A. Lourenço, Artur B. Cardoso, Eugénia Álvaro, Pedro Silva, Marco Coelho, Norberta Vilaça, João Meireles, Fátima Brôco, Nuno Carvalho, Marta Santos, Ricardo |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Monteiro, Sílvia Rente, Daniela Cunha, Mónica V. Gomes, Manuel Carmo Marques, Tiago A. Lourenço, Artur B. Cardoso, Eugénia Álvaro, Pedro Silva, Marco Coelho, Norberta Vilaça, João Meireles, Fátima Brôco, Nuno Carvalho, Marta Santos, Ricardo |
description |
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater produced interest in its use for sentinel surveillance at a community level and as a complementary approach to syndromic surveillance. With this work, we set the foundations for wastewater-based epidemiology (WBE) in Portugal by monitoring the trends of SARS-CoV-2 RNA circulation in the community, on a nationwide perspective during different epidemiological phases of the pandemic. The Charité assays (E_Sarbecco, RdRP, and N_Sarbecco) were applied to monitor, over 32-weeks (April to December 2020), the dynamics of SARS-CoV-2 RNA at the inlet of five wastewater treatment plants (WWTP), which together serve more than two million people in Portugal. Raw wastewater from three Coronavirus disease 2019 (COVID-19) reference hospitals was also analyzed during this period. In total, more than 600 samples were tested. For the first weeks, detection of SARS-CoV-2 RNA was sporadic, with concentrations varying from 103 to 105 genome copies per liter (GC/L). Prevalence of SARS-CoV-2 RNA increased steeply by the end of May into late June, mainly in Lisboa e Vale do Tejo region (LVT), during the reopening phase. After the summer, with the reopening of schools in mid-September and return to partial face-to-face work, a pronounced increase of SARS-CoV-2 RNA in wastewater was detected. In the LVT area, SARS-CoV-2 RNA load agreed with reported trends in hotspots of infection. Synchrony between trends of SARS-CoV-2 RNA in raw wastewater and daily new COVID-19 cases highlights the value of WBE as a surveillance tool, particularly after the phasing out of the epidemiological curve and when hotspots of disease re-emerge in the population which might be difficult to spot based solely on syndromic surveillance and contact tracing. This is the first study crossing several epidemiological stages highlighting the long-term use of WBE for SARS-CoV-2. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01 2022-01-01T00:00:00Z 2024-01-01T01:31:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/51846 |
url |
http://hdl.handle.net/10451/51846 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sílvia Monteiro, Daniela Rente, Mónica V. Cunha, Manuel Carmo Gomes, Tiago A. Marques, Artur B. Lourenço, Eugénia Cardoso, Pedro Álvaro, Marco Silva, Norberta Coelho, João Vilaça, Fátima Meireles, Nuno Brôco, Marta Carvalho, Ricardo Santos, A wastewater-based epidemiology tool for COVID-19 surveillance in Portugal, Science of The Total Environment, Volume 804, 2022, 150264, ISSN 0048-9697, https://doi.org/10.1016/j.scitotenv.2021.150264. 10.1016/j.scitotenv.2021.150264 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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