Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

Detalhes bibliográficos
Autor(a) principal: Cunha, Ana
Data de Publicação: 1997
Outros Autores: Ferreira, Manuel Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/3000
Resumo: A single medium was used for induction of somatic embryogenesis and shoot organogenesis from flax hypocotyls. With this system, a comparative study was performed to determine the free sterols content and composition in non-organogenic, shoot organogenic and embryogenic calli as well as in regenerated shoots and somatic embryos grown under the same hormonal supplementation. The induction of somatic embryogenesis and shoot organogenesis of flax was associated with an increase of total sterols in the competent calli and an increased ratio of stigmasterol to β-sitosterol in derived embryos and shoots. On the contrary, in non-organogenic calli, the ratio stigmasterol to β-sitosterol decreased during the exponential growth phase due to a drastic rise in the β-sitosterol content. This effect as well as the calli growth were higher with 0.5 mg/l 2,4-D than with 0.6 mg/l IBA.
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spelling Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flaxFree sterolsLipid contentIn vitro tissue cultureSomatic embryosLinum usitatissimumScience & TechnologyA single medium was used for induction of somatic embryogenesis and shoot organogenesis from flax hypocotyls. With this system, a comparative study was performed to determine the free sterols content and composition in non-organogenic, shoot organogenic and embryogenic calli as well as in regenerated shoots and somatic embryos grown under the same hormonal supplementation. The induction of somatic embryogenesis and shoot organogenesis of flax was associated with an increase of total sterols in the competent calli and an increased ratio of stigmasterol to β-sitosterol in derived embryos and shoots. On the contrary, in non-organogenic calli, the ratio stigmasterol to β-sitosterol decreased during the exponential growth phase due to a drastic rise in the β-sitosterol content. This effect as well as the calli growth were higher with 0.5 mg/l 2,4-D than with 0.6 mg/l IBA.Junta Nacional de Investigação Científica e Tecnológica (JNICT) - BD/2771/93.ElsevierUniversidade do MinhoCunha, AnaFerreira, Manuel Fernandes1997-041997-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/3000eng"Plant Science". ISSN 0168-9452. 124:1 (1997) 97-105.0168-945210.1016/S0168-9452(97)04587-1http://www.elsevier.com/wps/find/journaldescription.cws_home/506030/description#descriptioninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:11:28Zoai:repositorium.sdum.uminho.pt:1822/3000Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:11:28Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
title Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
spellingShingle Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
Cunha, Ana
Free sterols
Lipid content
In vitro tissue culture
Somatic embryos
Linum usitatissimum
Science & Technology
title_short Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
title_full Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
title_fullStr Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
title_full_unstemmed Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
title_sort Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax
author Cunha, Ana
author_facet Cunha, Ana
Ferreira, Manuel Fernandes
author_role author
author2 Ferreira, Manuel Fernandes
author2_role author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cunha, Ana
Ferreira, Manuel Fernandes
dc.subject.por.fl_str_mv Free sterols
Lipid content
In vitro tissue culture
Somatic embryos
Linum usitatissimum
Science & Technology
topic Free sterols
Lipid content
In vitro tissue culture
Somatic embryos
Linum usitatissimum
Science & Technology
description A single medium was used for induction of somatic embryogenesis and shoot organogenesis from flax hypocotyls. With this system, a comparative study was performed to determine the free sterols content and composition in non-organogenic, shoot organogenic and embryogenic calli as well as in regenerated shoots and somatic embryos grown under the same hormonal supplementation. The induction of somatic embryogenesis and shoot organogenesis of flax was associated with an increase of total sterols in the competent calli and an increased ratio of stigmasterol to β-sitosterol in derived embryos and shoots. On the contrary, in non-organogenic calli, the ratio stigmasterol to β-sitosterol decreased during the exponential growth phase due to a drastic rise in the β-sitosterol content. This effect as well as the calli growth were higher with 0.5 mg/l 2,4-D than with 0.6 mg/l IBA.
publishDate 1997
dc.date.none.fl_str_mv 1997-04
1997-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/3000
url http://hdl.handle.net/1822/3000
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Plant Science". ISSN 0168-9452. 124:1 (1997) 97-105.
0168-9452
10.1016/S0168-9452(97)04587-1
http://www.elsevier.com/wps/find/journaldescription.cws_home/506030/description#description
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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