Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/11326 |
Resumo: | Lactose is an interesting carbon source for the production of several bio-products by fermentation, primarily because it is the major component of cheese whey, the main by-product of dairy activities. However, the microorganism more widely used in industrial fermentation processes, the yeast Saccharomyces cerevisiae, does not have a lactose metabolisation system. Therefore, several metabolic engineering approaches have been used to construct lactose-consuming S. cerevisiae strains, particularly involving the expression of the lactose genes of the phylogenetically related yeast Kluyveromyces lactis, but also the lactose genes from Escherichia coli and Aspergillus niger, as reviewed here. Due to the existing large amounts of whey, the production of bio-ethanol from lactose by engineered S. cerevisiae has been considered as a possible route for whey surplus. Emphasis is given in the present review on strain improvement for lactose-to-ethanol bioprocesses, namely flocculent yeast strains for continuous high-cell-density systems with enhanced ethanol productivity. |
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Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentationCheese wheyLactoseRecombinant Saccharomyces cerevisiaeBio-ethanolFermentationMetabolic engineeringLactose is an interesting carbon source for the production of several bio-products by fermentation, primarily because it is the major component of cheese whey, the main by-product of dairy activities. However, the microorganism more widely used in industrial fermentation processes, the yeast Saccharomyces cerevisiae, does not have a lactose metabolisation system. Therefore, several metabolic engineering approaches have been used to construct lactose-consuming S. cerevisiae strains, particularly involving the expression of the lactose genes of the phylogenetically related yeast Kluyveromyces lactis, but also the lactose genes from Escherichia coli and Aspergillus niger, as reviewed here. Due to the existing large amounts of whey, the production of bio-ethanol from lactose by engineered S. cerevisiae has been considered as a possible route for whey surplus. Emphasis is given in the present review on strain improvement for lactose-to-ethanol bioprocesses, namely flocculent yeast strains for continuous high-cell-density systems with enhanced ethanol productivity.Fundação para a Ciência e a Tecnologia (FCT)Landes BioscienceUniversidade do MinhoDomingues, LucíliaGuimarães, Pedro M. R.Oliveira, Carla Cristina Marques de2010-052010-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/11326eng"Bioengineered Bugs." ISSN 1949-1018. 1:3 (Maio/Jun. 2010) 1-8.1949-101810.4161/bbug.1.3.1061921326922http://www.landesbioscience.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:08Zoai:repositorium.sdum.uminho.pt:1822/11326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:19.285301Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
title |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
spellingShingle |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation Domingues, Lucília Cheese whey Lactose Recombinant Saccharomyces cerevisiae Bio-ethanol Fermentation Metabolic engineering |
title_short |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
title_full |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
title_fullStr |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
title_full_unstemmed |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
title_sort |
Metabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation |
author |
Domingues, Lucília |
author_facet |
Domingues, Lucília Guimarães, Pedro M. R. Oliveira, Carla Cristina Marques de |
author_role |
author |
author2 |
Guimarães, Pedro M. R. Oliveira, Carla Cristina Marques de |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Domingues, Lucília Guimarães, Pedro M. R. Oliveira, Carla Cristina Marques de |
dc.subject.por.fl_str_mv |
Cheese whey Lactose Recombinant Saccharomyces cerevisiae Bio-ethanol Fermentation Metabolic engineering |
topic |
Cheese whey Lactose Recombinant Saccharomyces cerevisiae Bio-ethanol Fermentation Metabolic engineering |
description |
Lactose is an interesting carbon source for the production of several bio-products by fermentation, primarily because it is the major component of cheese whey, the main by-product of dairy activities. However, the microorganism more widely used in industrial fermentation processes, the yeast Saccharomyces cerevisiae, does not have a lactose metabolisation system. Therefore, several metabolic engineering approaches have been used to construct lactose-consuming S. cerevisiae strains, particularly involving the expression of the lactose genes of the phylogenetically related yeast Kluyveromyces lactis, but also the lactose genes from Escherichia coli and Aspergillus niger, as reviewed here. Due to the existing large amounts of whey, the production of bio-ethanol from lactose by engineered S. cerevisiae has been considered as a possible route for whey surplus. Emphasis is given in the present review on strain improvement for lactose-to-ethanol bioprocesses, namely flocculent yeast strains for continuous high-cell-density systems with enhanced ethanol productivity. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-05 2010-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/11326 |
url |
http://hdl.handle.net/1822/11326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Bioengineered Bugs." ISSN 1949-1018. 1:3 (Maio/Jun. 2010) 1-8. 1949-1018 10.4161/bbug.1.3.10619 21326922 http://www.landesbioscience.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Landes Bioscience |
publisher.none.fl_str_mv |
Landes Bioscience |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132933406064640 |