A study on the geochemical conditions in hydrothermal vents

Detalhes bibliográficos
Autor(a) principal: Pereira, Delfina Patrícia Henriques
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/48120
Resumo: Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2020
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spelling A study on the geochemical conditions in hydrothermal ventsFontes hidrotermaisestrutura microporosaorigem do metabolismoreduçãomagnetiteTeses de mestrado - 2020Departamento de Biologia VegetalTese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2020Alkaline hydrothermal vents are ancient ocean structures, H2-producing, with pH, temperature and redox gradients which promote reduction and have been of great interest for Origin of Life researchers10,23,24. In the laboratory, CO2 can be reduced under hydrothermal conditions to organic molecules, such as ethanol, formate, methane, acetate and pyruvate 255,6. These molecules are also part of the ancient acetyl-CoA pathway, an exergonic, H2-dependent, CO2 reducing metabolic pathway, which possibly dates back to the Last Universal Common Ancestor (LUCA)3,26. In addition, the minerals within alkaline hydrothermal vents are rich in iron and nickel, two metals that are commonly found in the catalytic centers of modern-day enzymes, including the acetyl-CoA pathway27. They were also essential to catalyze CO2 reduction to synthesize more complex molecules such as pyruvate in the laboratory28,29. The idea that alkaline hydrothermal vents can be associated to an ancient metabolic pathway leads to an interesting argument for the location of the origin of metabolism. In this work I looked to expand the range of studies with hydrothermal conditions to new topics beyond CO2 reduction, exploring the effects of the complex microporous structure of these vents and if such rudimentary conditions could support modern day coenzymes and its redox reactions. For the first study an open microporous framework with zeolite and Laponite-RD was developed to simulate the vents’ structure and reproduced previous CO2 reduction experiments in this new setting. Secondly, the stability and reduction of nicotinamide adenine dinucleotide (NAD) under hydrothermal conditions was studied, an ancient coenzyme essential for metabolism.Results suggest that the framework used promotes the synthesis of more complex molecules such as pyruvate and acetate, in detriment of the production of simpler ones such as formate and that NAD is not only stable in alkaline fluids, but also can be reduced in the presence magnetite (Fe3O4). Reducing contamination and improving analytic methods are important next steps to take for this work, which already presents an avenue that further connects alkaline hydrothermal vents to the origin of metabolism.Martin, William F.Silva, Pedro João Neves e,1958-Repositório da Universidade de LisboaPereira, Delfina Patrícia Henriques2021-05-24T16:23:14Z202020202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/48120TID:202695735enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:22Zoai:repositorio.ul.pt:10451/48120Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:02.637795Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A study on the geochemical conditions in hydrothermal vents
title A study on the geochemical conditions in hydrothermal vents
spellingShingle A study on the geochemical conditions in hydrothermal vents
Pereira, Delfina Patrícia Henriques
Fontes hidrotermais
estrutura microporosa
origem do metabolismo
redução
magnetite
Teses de mestrado - 2020
Departamento de Biologia Vegetal
title_short A study on the geochemical conditions in hydrothermal vents
title_full A study on the geochemical conditions in hydrothermal vents
title_fullStr A study on the geochemical conditions in hydrothermal vents
title_full_unstemmed A study on the geochemical conditions in hydrothermal vents
title_sort A study on the geochemical conditions in hydrothermal vents
author Pereira, Delfina Patrícia Henriques
author_facet Pereira, Delfina Patrícia Henriques
author_role author
dc.contributor.none.fl_str_mv Martin, William F.
Silva, Pedro João Neves e,1958-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Pereira, Delfina Patrícia Henriques
dc.subject.por.fl_str_mv Fontes hidrotermais
estrutura microporosa
origem do metabolismo
redução
magnetite
Teses de mestrado - 2020
Departamento de Biologia Vegetal
topic Fontes hidrotermais
estrutura microporosa
origem do metabolismo
redução
magnetite
Teses de mestrado - 2020
Departamento de Biologia Vegetal
description Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2020
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020-01-01T00:00:00Z
2021-05-24T16:23:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/48120
TID:202695735
url http://hdl.handle.net/10451/48120
identifier_str_mv TID:202695735
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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