A study on the geochemical conditions in hydrothermal vents
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/48120 |
Resumo: | Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2020 |
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A study on the geochemical conditions in hydrothermal ventsFontes hidrotermaisestrutura microporosaorigem do metabolismoreduçãomagnetiteTeses de mestrado - 2020Departamento de Biologia VegetalTese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2020Alkaline hydrothermal vents are ancient ocean structures, H2-producing, with pH, temperature and redox gradients which promote reduction and have been of great interest for Origin of Life researchers10,23,24. In the laboratory, CO2 can be reduced under hydrothermal conditions to organic molecules, such as ethanol, formate, methane, acetate and pyruvate 255,6. These molecules are also part of the ancient acetyl-CoA pathway, an exergonic, H2-dependent, CO2 reducing metabolic pathway, which possibly dates back to the Last Universal Common Ancestor (LUCA)3,26. In addition, the minerals within alkaline hydrothermal vents are rich in iron and nickel, two metals that are commonly found in the catalytic centers of modern-day enzymes, including the acetyl-CoA pathway27. They were also essential to catalyze CO2 reduction to synthesize more complex molecules such as pyruvate in the laboratory28,29. The idea that alkaline hydrothermal vents can be associated to an ancient metabolic pathway leads to an interesting argument for the location of the origin of metabolism. In this work I looked to expand the range of studies with hydrothermal conditions to new topics beyond CO2 reduction, exploring the effects of the complex microporous structure of these vents and if such rudimentary conditions could support modern day coenzymes and its redox reactions. For the first study an open microporous framework with zeolite and Laponite-RD was developed to simulate the vents’ structure and reproduced previous CO2 reduction experiments in this new setting. Secondly, the stability and reduction of nicotinamide adenine dinucleotide (NAD) under hydrothermal conditions was studied, an ancient coenzyme essential for metabolism.Results suggest that the framework used promotes the synthesis of more complex molecules such as pyruvate and acetate, in detriment of the production of simpler ones such as formate and that NAD is not only stable in alkaline fluids, but also can be reduced in the presence magnetite (Fe3O4). Reducing contamination and improving analytic methods are important next steps to take for this work, which already presents an avenue that further connects alkaline hydrothermal vents to the origin of metabolism.Martin, William F.Silva, Pedro João Neves e,1958-Repositório da Universidade de LisboaPereira, Delfina Patrícia Henriques2021-05-24T16:23:14Z202020202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/48120TID:202695735enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:51:22Zoai:repositorio.ul.pt:10451/48120Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:02.637795Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A study on the geochemical conditions in hydrothermal vents |
title |
A study on the geochemical conditions in hydrothermal vents |
spellingShingle |
A study on the geochemical conditions in hydrothermal vents Pereira, Delfina Patrícia Henriques Fontes hidrotermais estrutura microporosa origem do metabolismo redução magnetite Teses de mestrado - 2020 Departamento de Biologia Vegetal |
title_short |
A study on the geochemical conditions in hydrothermal vents |
title_full |
A study on the geochemical conditions in hydrothermal vents |
title_fullStr |
A study on the geochemical conditions in hydrothermal vents |
title_full_unstemmed |
A study on the geochemical conditions in hydrothermal vents |
title_sort |
A study on the geochemical conditions in hydrothermal vents |
author |
Pereira, Delfina Patrícia Henriques |
author_facet |
Pereira, Delfina Patrícia Henriques |
author_role |
author |
dc.contributor.none.fl_str_mv |
Martin, William F. Silva, Pedro João Neves e,1958- Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Pereira, Delfina Patrícia Henriques |
dc.subject.por.fl_str_mv |
Fontes hidrotermais estrutura microporosa origem do metabolismo redução magnetite Teses de mestrado - 2020 Departamento de Biologia Vegetal |
topic |
Fontes hidrotermais estrutura microporosa origem do metabolismo redução magnetite Teses de mestrado - 2020 Departamento de Biologia Vegetal |
description |
Tese de mestrado, Biologia Molecular e Genética, Universidade de Lisboa, Faculdade de Ciências, 2020 |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020 2020-01-01T00:00:00Z 2021-05-24T16:23:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/48120 TID:202695735 |
url |
http://hdl.handle.net/10451/48120 |
identifier_str_mv |
TID:202695735 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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