Lipocalin 2 is present in the EAE brain and is modulated by natalizumab
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/21071 |
Resumo: | The authors acknowledge the BiogenIdec, for providing Natalizumab (BiogenIdec, Boston, MA, USA). We are thankful to theCOST(European Cooperation in Science and Technology) Action NEURINFNETBM0603. We also thank Dr. Nadine Santos for critically reviewing this manuscript. |
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oai:repositorium.sdum.uminho.pt:1822/21071 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Lipocalin 2 is present in the EAE brain and is modulated by natalizumabMultiple sclerosisLipocalin 2Experimental autoimmune encephalomyelitisAstrocytes,Cerebrospinal fluidNatalizumabScience & TechnologyThe authors acknowledge the BiogenIdec, for providing Natalizumab (BiogenIdec, Boston, MA, USA). We are thankful to theCOST(European Cooperation in Science and Technology) Action NEURINFNETBM0603. We also thank Dr. Nadine Santos for critically reviewing this manuscript.Multiple sclerosis (MS) is a demyelinating disease that causes major neurological disability in young adults. A definitive diagnosis at the time of the first episode is still lacking, but since early treatment leads to better prognosis, the search for early biomarkers is needed. Here we characterized the transcriptome of the choroid plexus (CP), which is part of the blood-brain barriers (BBBs) and the major site of cerebrospinal fluid production, in the experimental autoimmune encephalomyelitis (EAE) mouse model of MS. In addition, cerebrospinal fluid samples from two cohorts of patients with MS and with optic neuritis (ON) were analyzed to confirm the clinical relevance of the findings. Genes encoding for adhesion molecules, chemokines and cytokines displayed the most altered expression, supporting the role of CP as a site of immune-brain interaction in MS. The gene encoding for lipocalin 2 was the most up-regulated; notably, the cerebrospinal fluid lipocalin 2 levels coincided with the active phases of the disease. Immunostaining revealed that neutrophils infiltrating the CP were the source of the increased lipocalin 2 expression in this structure. However, within the brain, lipocalin 2 was also detected in astrocytes, particularly in regions typically affected in patients with MS. The increase of lipocalin 2 in the cerebrospinal fluid and in astrocytes was reverted by natalizumab treatment. Most importantly, the results obtained in the murine model were translatable into humans since patients from two different cohorts presented increased cerebrospinal fluid lipocalin 2 levels. The findings support lipocalin 2 as a valuable molecule for the diagnostic/monitoring panel of MS.This work was supported by a grant from The Dana Foundation (USA) and by a grant from Fundação para a Ciência e Tecnologia(FCT, Portugal) (PIC/IC/83231/2007). Fernanda Marques and Sandro D. Mesquita are recipients of postdoctoral and doctoral fellow- ships from FCT, Portugal, respectively.Frontiers MediaUniversidade do MinhoMarques, FernandaMesquita, SandroSousa, JoãoCoppola, GiovanniGao, FuyingGeschwind, Daniel H.Columba-Cabezas, SandraAloisi, FrancescaDegn, MatildaCerqueira, JoãoSousa, NunoNeves, Margarida CorreiaPalha, Joana Almeida2012-11-292012-11-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/21071eng1662-510210.3389/fncel.2012.00033info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:24Zoai:repositorium.sdum.uminho.pt:1822/21071Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:42.096316Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| title |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| spellingShingle |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab Marques, Fernanda Multiple sclerosis Lipocalin 2 Experimental autoimmune encephalomyelitis Astrocytes, Cerebrospinal fluid Natalizumab Science & Technology |
| title_short |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| title_full |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| title_fullStr |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| title_full_unstemmed |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| title_sort |
Lipocalin 2 is present in the EAE brain and is modulated by natalizumab |
| author |
Marques, Fernanda |
| author_facet |
Marques, Fernanda Mesquita, Sandro Sousa, João Coppola, Giovanni Gao, Fuying Geschwind, Daniel H. Columba-Cabezas, Sandra Aloisi, Francesca Degn, Matilda Cerqueira, João Sousa, Nuno Neves, Margarida Correia Palha, Joana Almeida |
| author_role |
author |
| author2 |
Mesquita, Sandro Sousa, João Coppola, Giovanni Gao, Fuying Geschwind, Daniel H. Columba-Cabezas, Sandra Aloisi, Francesca Degn, Matilda Cerqueira, João Sousa, Nuno Neves, Margarida Correia Palha, Joana Almeida |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Marques, Fernanda Mesquita, Sandro Sousa, João Coppola, Giovanni Gao, Fuying Geschwind, Daniel H. Columba-Cabezas, Sandra Aloisi, Francesca Degn, Matilda Cerqueira, João Sousa, Nuno Neves, Margarida Correia Palha, Joana Almeida |
| dc.subject.por.fl_str_mv |
Multiple sclerosis Lipocalin 2 Experimental autoimmune encephalomyelitis Astrocytes, Cerebrospinal fluid Natalizumab Science & Technology |
| topic |
Multiple sclerosis Lipocalin 2 Experimental autoimmune encephalomyelitis Astrocytes, Cerebrospinal fluid Natalizumab Science & Technology |
| description |
The authors acknowledge the BiogenIdec, for providing Natalizumab (BiogenIdec, Boston, MA, USA). We are thankful to theCOST(European Cooperation in Science and Technology) Action NEURINFNETBM0603. We also thank Dr. Nadine Santos for critically reviewing this manuscript. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-11-29 2012-11-29T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/21071 |
| url |
http://hdl.handle.net/1822/21071 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1662-5102 10.3389/fncel.2012.00033 |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
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Frontiers Media |
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Frontiers Media |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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